Extracts of Amazonian Fungi With Larvicidal Activities Against Aedes aegypti
The global increase in diseases transmitted by the vector Aedes aegypti, new and re-emerging, underscores the need for alternative and more effective methods of controlling mosquitoes. Our aim was to identify fungal strains from the Amazon rain forest that produce metabolites with larvicidal activit...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.743246/full |
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| author | Marta Rodrigues de Oliveira Ricardo de Melo Katak Gilvan Ferreira da Silva Osvaldo Marinotti Olle Terenius Wanderli Pedro Tadei Wanderli Pedro Tadei Wanderli Pedro Tadei Afonso Duarte Leão de Souza Afonso Duarte Leão de Souza Afonso Duarte Leão de Souza Antonia Queiroz Lima de Souza Antonia Queiroz Lima de Souza Antonia Queiroz Lima de Souza |
| author_facet | Marta Rodrigues de Oliveira Ricardo de Melo Katak Gilvan Ferreira da Silva Osvaldo Marinotti Olle Terenius Wanderli Pedro Tadei Wanderli Pedro Tadei Wanderli Pedro Tadei Afonso Duarte Leão de Souza Afonso Duarte Leão de Souza Afonso Duarte Leão de Souza Antonia Queiroz Lima de Souza Antonia Queiroz Lima de Souza Antonia Queiroz Lima de Souza |
| author_sort | Marta Rodrigues de Oliveira |
| collection | DOAJ |
| description | The global increase in diseases transmitted by the vector Aedes aegypti, new and re-emerging, underscores the need for alternative and more effective methods of controlling mosquitoes. Our aim was to identify fungal strains from the Amazon rain forest that produce metabolites with larvicidal activity against Aedes aegypti. Thirty-six fungal strains belonging to 23 different genera of fungi, isolated from water samples collected in the state of Amazonas, Brazil were cultivated. The liquid medium was separated from the mycelium by filtration. Medium fractions were extracted with ethyl acetate and isopropanol 9:1 volume:volume, and the mycelia with ethyl acetate and methanol 1:1. The extracts were vacuum dried and the larvicidal activity was evaluated in selective bioassays containing 500 μg/ml of the dried fungal extracts. Larval mortality was evaluated up to 72 h. None of the mycelium extracts showed larvicidal activity greater than 50% at 72 h. In contrast, 15 culture medium extracts had larvicidal activity equal to or greater than 50% and eight killed more than 90% of the larvae within 72 h. These eight extracts from fungi belonging to seven different genera (Aspergillus, Cladosporium, Trichoderma, Diaporthe, Albifimbria, Emmia, and Sarocladium) were selected for the determination of LC50 and LC90. Albifimbria lateralis (1160) medium extracts presented the lowest LC50 value (0.268 μg/ml) after 24 h exposure. Diaporthe ueckerae (1203) medium extracts presented the lowest value of LC90 (2.928 μg/ml) at 24 h, the lowest values of LC50 (0.108 μg/ml) and LC90 (0.894 μg/ml) at 48 h and also at 72 h (LC50 = 0.062 μg/ml and LC90 = 0.476 μg/ml). Extracts from Al. lateralis (1160) and D. ueckerae (1203) showed potential for developing new, naturally derived products, to be applied in integrated vector management programs against Ae. aegypti. |
| first_indexed | 2024-12-14T23:24:13Z |
| format | Article |
| id | doaj.art-c2c66c7a8e04411d9eb4a7ecdf05a2cb |
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| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-14T23:24:13Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-c2c66c7a8e04411d9eb4a7ecdf05a2cb2022-12-21T22:43:51ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-12-011210.3389/fmicb.2021.743246743246Extracts of Amazonian Fungi With Larvicidal Activities Against Aedes aegyptiMarta Rodrigues de Oliveira0Ricardo de Melo Katak1Gilvan Ferreira da Silva2Osvaldo Marinotti3Olle Terenius4Wanderli Pedro Tadei5Wanderli Pedro Tadei6Wanderli Pedro Tadei7Afonso Duarte Leão de Souza8Afonso Duarte Leão de Souza9Afonso Duarte Leão de Souza10Antonia Queiroz Lima de Souza11Antonia Queiroz Lima de Souza12Antonia Queiroz Lima de Souza13Programa de Pós-graduação em Biodiversidade e Biotecnologia (PPG-BIONORTE), Universidade Federal do Amazonas, Manaus, BrazilPrograma de Pós-graduação em Biotecnologia, Universidade Federal do Amazonas, Manaus, BrazilEmbrapa Amazônia Ocidental, Manaus, BrazilMTEKPrime, Aliso Viejo, CA, United StatesDepartment of Cell and Molecular Biology, Microbiology, Uppsala University, Uppsala, SwedenPrograma de Pós-graduação em Biodiversidade e Biotecnologia (PPG-BIONORTE), Universidade Federal do Amazonas, Manaus, BrazilPrograma de Pós-graduação em Biotecnologia, Universidade Federal do Amazonas, Manaus, BrazilLaboratório de Malária e Dengue, Instituto Nacional de Pesquisas da Amazônia, Manaus, BrazilPrograma de Pós-graduação em Biodiversidade e Biotecnologia (PPG-BIONORTE), Universidade Federal do Amazonas, Manaus, BrazilCentral Analítica – Centro de Apoio Multidisciplinar, Universidade Federal do Amazonas, Manaus, BrazilDepartamento de Química, Universidade Federal do Amazonas, Manaus, BrazilPrograma de Pós-graduação em Biodiversidade e Biotecnologia (PPG-BIONORTE), Universidade Federal do Amazonas, Manaus, BrazilCentral Analítica – Centro de Apoio Multidisciplinar, Universidade Federal do Amazonas, Manaus, BrazilFaculdade de Ciências Agrárias, Universidade Federal do Amazonas, Manaus, BrazilThe global increase in diseases transmitted by the vector Aedes aegypti, new and re-emerging, underscores the need for alternative and more effective methods of controlling mosquitoes. Our aim was to identify fungal strains from the Amazon rain forest that produce metabolites with larvicidal activity against Aedes aegypti. Thirty-six fungal strains belonging to 23 different genera of fungi, isolated from water samples collected in the state of Amazonas, Brazil were cultivated. The liquid medium was separated from the mycelium by filtration. Medium fractions were extracted with ethyl acetate and isopropanol 9:1 volume:volume, and the mycelia with ethyl acetate and methanol 1:1. The extracts were vacuum dried and the larvicidal activity was evaluated in selective bioassays containing 500 μg/ml of the dried fungal extracts. Larval mortality was evaluated up to 72 h. None of the mycelium extracts showed larvicidal activity greater than 50% at 72 h. In contrast, 15 culture medium extracts had larvicidal activity equal to or greater than 50% and eight killed more than 90% of the larvae within 72 h. These eight extracts from fungi belonging to seven different genera (Aspergillus, Cladosporium, Trichoderma, Diaporthe, Albifimbria, Emmia, and Sarocladium) were selected for the determination of LC50 and LC90. Albifimbria lateralis (1160) medium extracts presented the lowest LC50 value (0.268 μg/ml) after 24 h exposure. Diaporthe ueckerae (1203) medium extracts presented the lowest value of LC90 (2.928 μg/ml) at 24 h, the lowest values of LC50 (0.108 μg/ml) and LC90 (0.894 μg/ml) at 48 h and also at 72 h (LC50 = 0.062 μg/ml and LC90 = 0.476 μg/ml). Extracts from Al. lateralis (1160) and D. ueckerae (1203) showed potential for developing new, naturally derived products, to be applied in integrated vector management programs against Ae. aegypti.https://www.frontiersin.org/articles/10.3389/fmicb.2021.743246/fullbiological controlarbovirusAedes aegyptilarvicidal activitymetabolites |
| spellingShingle | Marta Rodrigues de Oliveira Ricardo de Melo Katak Gilvan Ferreira da Silva Osvaldo Marinotti Olle Terenius Wanderli Pedro Tadei Wanderli Pedro Tadei Wanderli Pedro Tadei Afonso Duarte Leão de Souza Afonso Duarte Leão de Souza Afonso Duarte Leão de Souza Antonia Queiroz Lima de Souza Antonia Queiroz Lima de Souza Antonia Queiroz Lima de Souza Extracts of Amazonian Fungi With Larvicidal Activities Against Aedes aegypti Frontiers in Microbiology biological control arbovirus Aedes aegypti larvicidal activity metabolites |
| title | Extracts of Amazonian Fungi With Larvicidal Activities Against Aedes aegypti |
| title_full | Extracts of Amazonian Fungi With Larvicidal Activities Against Aedes aegypti |
| title_fullStr | Extracts of Amazonian Fungi With Larvicidal Activities Against Aedes aegypti |
| title_full_unstemmed | Extracts of Amazonian Fungi With Larvicidal Activities Against Aedes aegypti |
| title_short | Extracts of Amazonian Fungi With Larvicidal Activities Against Aedes aegypti |
| title_sort | extracts of amazonian fungi with larvicidal activities against aedes aegypti |
| topic | biological control arbovirus Aedes aegypti larvicidal activity metabolites |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.743246/full |
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