Evaluation of Genetic Diversity in Japanese and English White Quail Populations Using Microsatellite Markers
The Japanese and English White quails are widespread strains and belongs to the Galliformes order, Phasianidae family, Coturnix genus and Japonica species. These birds are likely to be well-adapted to the hard conditions and resistance to diseases as it has attained economic importance as an agricu...
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| Format: | Article |
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Gorgan University of Agricultural Sciences and Natural Resources
2013-01-01
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| Series: | Poultry Science Journal |
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| Online Access: | http://psj.gau.ac.ir/pdf_1472_181a67de4e05393f9a15b3e7ea30b08e.html |
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| author | Mamizade N Ahani Azari M Zerehdaran S Khan Ahmadi AR Naghavian S |
| author_facet | Mamizade N Ahani Azari M Zerehdaran S Khan Ahmadi AR Naghavian S |
| author_sort | Mamizade N |
| collection | DOAJ |
| description | The Japanese and English White quails are widespread strains and belongs to the Galliformes order, Phasianidae family, Coturnix genus and Japonica species. These birds are likely to be well-adapted to the hard conditions and resistance to diseases as it has attained economic importance as an agricultural species. In the current study, the genetic variation of Japanese and English White quail populations were studied. Frequency of polymorphic loci, polymorphic information content, heterozygosity, Shannon's information index, number of observed and effective alleles were assessed using 4 microsatellite markers with high polymorphic information content value (GUJ0034, GUJ0049, GUJ0080 and GUJ0097). The Blood samples were collected randomly from 50 Japanese quails and 50 English White quails rearing in the research center of Gorgan University of Agricultural Sciences and Natural Resources. The genomic DNA was extracted using DIAtom DNA Prep 100 kit, and its quality and quantity were determined using electrophoresis gel and spectrophotometery methods. The PCR reactions were successfully performed with four microsatellite markers. The results based on the chi-square and likelihood ratio tests showed a significant deviation from Hardy-Weinberg equilibrium. The means of genetic diversity parameters such as number of effective alleles, the number of observed alleles, the expected and observed heterozygosity, Shannon's information index and PIC in quail populations were 4.78±0.37, 7.50±0.57, 0.79±0.02, 0.60±0.16, 1.73±0.05 and 0.76±0.02 respectively. The results of the current study showed that the investigated quail populations have a relatively high genetic diversity with respect to the applied microsatellite markers and confirmed prior study’s findings on the ability of microsatellite markers in investigating genetic diversity. |
| first_indexed | 2024-12-19T05:55:24Z |
| format | Article |
| id | doaj.art-c2c8f9ae46434a4e86ed0cfecf656ee6 |
| institution | Directory Open Access Journal |
| issn | 2345-6604 2345-6566 |
| language | English |
| last_indexed | 2024-12-19T05:55:24Z |
| publishDate | 2013-01-01 |
| publisher | Gorgan University of Agricultural Sciences and Natural Resources |
| record_format | Article |
| series | Poultry Science Journal |
| spelling | doaj.art-c2c8f9ae46434a4e86ed0cfecf656ee62022-12-21T20:33:28ZengGorgan University of Agricultural Sciences and Natural ResourcesPoultry Science Journal2345-66042345-65662013-01-011153621472Evaluation of Genetic Diversity in Japanese and English White Quail Populations Using Microsatellite MarkersMamizade N0Ahani Azari M1Zerehdaran S2Khan Ahmadi AR3Naghavian S4Faculty of Animal Science, Gorgan University of Agricultural Sciences and Natural ResourcesFaculty of Animal Science, Gorgan University of Agricultural Sciences and Natural ResourcesFaculty of Animal Science, Gorgan University of Agricultural Sciences and Natural ResourcesDepartment of Animal Science, Gonbad Kavous UniversityFaculty of Animal Science, Gorgan University of Agricultural Sciences and Natural ResourcesThe Japanese and English White quails are widespread strains and belongs to the Galliformes order, Phasianidae family, Coturnix genus and Japonica species. These birds are likely to be well-adapted to the hard conditions and resistance to diseases as it has attained economic importance as an agricultural species. In the current study, the genetic variation of Japanese and English White quail populations were studied. Frequency of polymorphic loci, polymorphic information content, heterozygosity, Shannon's information index, number of observed and effective alleles were assessed using 4 microsatellite markers with high polymorphic information content value (GUJ0034, GUJ0049, GUJ0080 and GUJ0097). The Blood samples were collected randomly from 50 Japanese quails and 50 English White quails rearing in the research center of Gorgan University of Agricultural Sciences and Natural Resources. The genomic DNA was extracted using DIAtom DNA Prep 100 kit, and its quality and quantity were determined using electrophoresis gel and spectrophotometery methods. The PCR reactions were successfully performed with four microsatellite markers. The results based on the chi-square and likelihood ratio tests showed a significant deviation from Hardy-Weinberg equilibrium. The means of genetic diversity parameters such as number of effective alleles, the number of observed alleles, the expected and observed heterozygosity, Shannon's information index and PIC in quail populations were 4.78±0.37, 7.50±0.57, 0.79±0.02, 0.60±0.16, 1.73±0.05 and 0.76±0.02 respectively. The results of the current study showed that the investigated quail populations have a relatively high genetic diversity with respect to the applied microsatellite markers and confirmed prior study’s findings on the ability of microsatellite markers in investigating genetic diversity.http://psj.gau.ac.ir/pdf_1472_181a67de4e05393f9a15b3e7ea30b08e.htmlJapanese quailHeterozygosityGenetic variationMicrosatellite marker |
| spellingShingle | Mamizade N Ahani Azari M Zerehdaran S Khan Ahmadi AR Naghavian S Evaluation of Genetic Diversity in Japanese and English White Quail Populations Using Microsatellite Markers Poultry Science Journal Japanese quail Heterozygosity Genetic variation Microsatellite marker |
| title | Evaluation of Genetic Diversity in Japanese and English White Quail Populations Using Microsatellite Markers |
| title_full | Evaluation of Genetic Diversity in Japanese and English White Quail Populations Using Microsatellite Markers |
| title_fullStr | Evaluation of Genetic Diversity in Japanese and English White Quail Populations Using Microsatellite Markers |
| title_full_unstemmed | Evaluation of Genetic Diversity in Japanese and English White Quail Populations Using Microsatellite Markers |
| title_short | Evaluation of Genetic Diversity in Japanese and English White Quail Populations Using Microsatellite Markers |
| title_sort | evaluation of genetic diversity in japanese and english white quail populations using microsatellite markers |
| topic | Japanese quail Heterozygosity Genetic variation Microsatellite marker |
| url | http://psj.gau.ac.ir/pdf_1472_181a67de4e05393f9a15b3e7ea30b08e.html |
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