Exogenous melatonin reduces the inhibitory effect of osmotic stress on photosynthesis in soybean.
Understanding the relationship between exogenous melatonin and water deficit stress is crucial for achieving high yields and alleviating the effects of water deficit stress on soybean (Glycine max (L.) Merrill) plants in agriculture. This study investigated the effects of exogenous melatonin on soyb...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0226542 |
| _version_ | 1818926942847500288 |
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| author | Mingcong Zhang Songyu He Yingce Zhan Bin Qin Xijun Jin Mengxue Wang Yuxian Zhang Guohua Hu Zhanlin Teng Yaokun Wu |
| author_facet | Mingcong Zhang Songyu He Yingce Zhan Bin Qin Xijun Jin Mengxue Wang Yuxian Zhang Guohua Hu Zhanlin Teng Yaokun Wu |
| author_sort | Mingcong Zhang |
| collection | DOAJ |
| description | Understanding the relationship between exogenous melatonin and water deficit stress is crucial for achieving high yields and alleviating the effects of water deficit stress on soybean (Glycine max (L.) Merrill) plants in agriculture. This study investigated the effects of exogenous melatonin on soybean photosynthetic capacity under water deficit stress induced by polyethylene glycol (PEG) 6000. We conducted a potting experiment in 2018 using the soybean (Glycine max L. Merrill) cultivar Suinong 26. We identified the impacts of a concentration of PEG 6000 simulating drought (15%, w/v) and an appropriate melatonin concentration (100 μmol/L) on the growth of soybean seedlings and flowering stages in a preliminary test. We applied exogenous melatonin by foliar spraying and root application to determine the effects on leaf photosynthesis during water deficit stress. Our results indicated that 15% PEG 6000 had an obvious inhibitory effect on the growth of soybean seedlings and flowering stages, causing oxidative stress and damage due to reactive oxygen species (ROS) (H2O2 and O2·-) accumulation and potentially reducing air exchange parameters and photosystem II (PSII) efficiency. The application of exogenous melatonin significantly relieved the inhibitory effects of PEG 6000 stress on seedlings and flowering growth, and gas exchange parameters, potentially improved PSII efficiency, improved the leaf area index (LAI) and the accumulation of dry matter, slowed down oxidative stress and damage to leaves by increasing the activity of antioxidant enzymes (SOD, POD, and CAT), reduced the content of malondialdehyde (MDA), and ultimately improved soybean yield. Overall, the results of this study demonstrated that application of exogenous melatonin at the seedlings and flowering stages of soybean is effective in alleviating plant damage caused by water deficit stress and improving the drought resistance of soybean plants. In addition, the results showed that application of exogenous melatonin by root is superior to foliar spraying. |
| first_indexed | 2024-12-20T03:05:08Z |
| format | Article |
| id | doaj.art-c2c9695908d2418d9e2a6a9e5fdf2b15 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T03:05:08Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c2c9695908d2418d9e2a6a9e5fdf2b152022-12-21T19:55:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-011412e022654210.1371/journal.pone.0226542Exogenous melatonin reduces the inhibitory effect of osmotic stress on photosynthesis in soybean.Mingcong ZhangSongyu HeYingce ZhanBin QinXijun JinMengxue WangYuxian ZhangGuohua HuZhanlin TengYaokun WuUnderstanding the relationship between exogenous melatonin and water deficit stress is crucial for achieving high yields and alleviating the effects of water deficit stress on soybean (Glycine max (L.) Merrill) plants in agriculture. This study investigated the effects of exogenous melatonin on soybean photosynthetic capacity under water deficit stress induced by polyethylene glycol (PEG) 6000. We conducted a potting experiment in 2018 using the soybean (Glycine max L. Merrill) cultivar Suinong 26. We identified the impacts of a concentration of PEG 6000 simulating drought (15%, w/v) and an appropriate melatonin concentration (100 μmol/L) on the growth of soybean seedlings and flowering stages in a preliminary test. We applied exogenous melatonin by foliar spraying and root application to determine the effects on leaf photosynthesis during water deficit stress. Our results indicated that 15% PEG 6000 had an obvious inhibitory effect on the growth of soybean seedlings and flowering stages, causing oxidative stress and damage due to reactive oxygen species (ROS) (H2O2 and O2·-) accumulation and potentially reducing air exchange parameters and photosystem II (PSII) efficiency. The application of exogenous melatonin significantly relieved the inhibitory effects of PEG 6000 stress on seedlings and flowering growth, and gas exchange parameters, potentially improved PSII efficiency, improved the leaf area index (LAI) and the accumulation of dry matter, slowed down oxidative stress and damage to leaves by increasing the activity of antioxidant enzymes (SOD, POD, and CAT), reduced the content of malondialdehyde (MDA), and ultimately improved soybean yield. Overall, the results of this study demonstrated that application of exogenous melatonin at the seedlings and flowering stages of soybean is effective in alleviating plant damage caused by water deficit stress and improving the drought resistance of soybean plants. In addition, the results showed that application of exogenous melatonin by root is superior to foliar spraying.https://doi.org/10.1371/journal.pone.0226542 |
| spellingShingle | Mingcong Zhang Songyu He Yingce Zhan Bin Qin Xijun Jin Mengxue Wang Yuxian Zhang Guohua Hu Zhanlin Teng Yaokun Wu Exogenous melatonin reduces the inhibitory effect of osmotic stress on photosynthesis in soybean. PLoS ONE |
| title | Exogenous melatonin reduces the inhibitory effect of osmotic stress on photosynthesis in soybean. |
| title_full | Exogenous melatonin reduces the inhibitory effect of osmotic stress on photosynthesis in soybean. |
| title_fullStr | Exogenous melatonin reduces the inhibitory effect of osmotic stress on photosynthesis in soybean. |
| title_full_unstemmed | Exogenous melatonin reduces the inhibitory effect of osmotic stress on photosynthesis in soybean. |
| title_short | Exogenous melatonin reduces the inhibitory effect of osmotic stress on photosynthesis in soybean. |
| title_sort | exogenous melatonin reduces the inhibitory effect of osmotic stress on photosynthesis in soybean |
| url | https://doi.org/10.1371/journal.pone.0226542 |
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