Green Synthesis of Zinc Oxide Nanocrystals Utilizing <i>Origanum majorana</i> Leaf Extract and Their Synergistic Patterns with Colistin against Multidrug-Resistant Bacterial Strains
There is a crucial necessity for the formulation of efficient antimicrobial agents owing to the increasing prevalence of hospital-acquired bacterial infections triggered by multidrug-resistant microbes that result in significant deaths and illnesses around the world. Hence, the current investigation...
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MDPI AG
2022-10-01
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| Series: | Crystals |
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| Online Access: | https://www.mdpi.com/2073-4352/12/11/1513 |
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| author | Mohamed Taha Yassin Abdulaziz Abdulrahman Al-Askar Khalid Maniah Fatimah O. Al-Otibi |
| author_facet | Mohamed Taha Yassin Abdulaziz Abdulrahman Al-Askar Khalid Maniah Fatimah O. Al-Otibi |
| author_sort | Mohamed Taha Yassin |
| collection | DOAJ |
| description | There is a crucial necessity for the formulation of efficient antimicrobial agents owing to the increasing prevalence of hospital-acquired bacterial infections triggered by multidrug-resistant microbes that result in significant deaths and illnesses around the world. Hence, the current investigation examined the antibacterial proficiency of zinc oxide nanoparticles formulated utilizing the green route against bacterial strains that were resistant to multiple drugs. In addition, the synergistic antibacterial action of ZnO nanoparticles (ZnO NPs) combined with colistin was investigated against the tested microbial strains to determine the efficiency of the bioinspired ZnO nanoparticles in boosting the antibacterial proficiency of colistin antibiotic. Incidentally, the bioinspired ZnO nanoparticles were synthesized using water extract of <i>Origanum majorana</i> leaves and these nanomaterials were physicochemically characterized using different analytical techniques. The bioactivity of the synthesized nanomaterials against multidrug-resistant bacterial strains was appraised using the agar diffusion method. The biogenic ZnO NPs at a concentration of 100 μg/disk revealed a compelling antimicrobial efficacy against the tested strains, expressing the maximum antimicrobial action against <i>Escherichia coli</i> strain with clear zone diameter of 38.16 ± 0.18 mm. The remarkable antibacterial proficiency might be accredited to the tiny particle size of the bioformulated ZnO NPs of 12.467 ± 1.36 nm. The net charge of ZnO nanomaterials was −14.8 mV while XRD analysis confirmed their hexagonal wurtzite structure. Furthermore, the bioformulated ZnO NPs showed a promising synergistic potency with colistin demonstrating respective synergism proportions of 91.05, 79.07, 75.04, 75.25, 56.28 and 10.60% against <i>E. coli</i>, <i>Klebsiella pneumoniae</i>, <i>Acinetobacter baumannii</i>, <i>Salmonella typhimurium</i>, <i>Enterobacter cloacae</i>, and <i>Pseudomonas aeruginosa,</i> respectively. In conclusion, the water extract of <i>O. majorana</i> leaves mediated green formulation of zinc oxide nanoparticles with unique physicochemical characteristics and effective antibacterial proficiency against the examined drug-resistant bacterial strains. These nanomaterials could be used in the synthesis of effective antibacterial coatings to control hospital acquired infections caused by multidrug-resistant bacterial pathogens. |
| first_indexed | 2024-03-09T19:10:40Z |
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| id | doaj.art-c2cab8ec0aed417cb74db6183deefb30 |
| institution | Directory Open Access Journal |
| issn | 2073-4352 |
| language | English |
| last_indexed | 2024-03-09T19:10:40Z |
| publishDate | 2022-10-01 |
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| record_format | Article |
| series | Crystals |
| spelling | doaj.art-c2cab8ec0aed417cb74db6183deefb302023-11-24T04:14:21ZengMDPI AGCrystals2073-43522022-10-011211151310.3390/cryst12111513Green Synthesis of Zinc Oxide Nanocrystals Utilizing <i>Origanum majorana</i> Leaf Extract and Their Synergistic Patterns with Colistin against Multidrug-Resistant Bacterial StrainsMohamed Taha Yassin0Abdulaziz Abdulrahman Al-Askar1Khalid Maniah2Fatimah O. Al-Otibi3Botany and Microbiology Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaBotany and Microbiology Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaBotany and Microbiology Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaBotany and Microbiology Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaThere is a crucial necessity for the formulation of efficient antimicrobial agents owing to the increasing prevalence of hospital-acquired bacterial infections triggered by multidrug-resistant microbes that result in significant deaths and illnesses around the world. Hence, the current investigation examined the antibacterial proficiency of zinc oxide nanoparticles formulated utilizing the green route against bacterial strains that were resistant to multiple drugs. In addition, the synergistic antibacterial action of ZnO nanoparticles (ZnO NPs) combined with colistin was investigated against the tested microbial strains to determine the efficiency of the bioinspired ZnO nanoparticles in boosting the antibacterial proficiency of colistin antibiotic. Incidentally, the bioinspired ZnO nanoparticles were synthesized using water extract of <i>Origanum majorana</i> leaves and these nanomaterials were physicochemically characterized using different analytical techniques. The bioactivity of the synthesized nanomaterials against multidrug-resistant bacterial strains was appraised using the agar diffusion method. The biogenic ZnO NPs at a concentration of 100 μg/disk revealed a compelling antimicrobial efficacy against the tested strains, expressing the maximum antimicrobial action against <i>Escherichia coli</i> strain with clear zone diameter of 38.16 ± 0.18 mm. The remarkable antibacterial proficiency might be accredited to the tiny particle size of the bioformulated ZnO NPs of 12.467 ± 1.36 nm. The net charge of ZnO nanomaterials was −14.8 mV while XRD analysis confirmed their hexagonal wurtzite structure. Furthermore, the bioformulated ZnO NPs showed a promising synergistic potency with colistin demonstrating respective synergism proportions of 91.05, 79.07, 75.04, 75.25, 56.28 and 10.60% against <i>E. coli</i>, <i>Klebsiella pneumoniae</i>, <i>Acinetobacter baumannii</i>, <i>Salmonella typhimurium</i>, <i>Enterobacter cloacae</i>, and <i>Pseudomonas aeruginosa,</i> respectively. In conclusion, the water extract of <i>O. majorana</i> leaves mediated green formulation of zinc oxide nanoparticles with unique physicochemical characteristics and effective antibacterial proficiency against the examined drug-resistant bacterial strains. These nanomaterials could be used in the synthesis of effective antibacterial coatings to control hospital acquired infections caused by multidrug-resistant bacterial pathogens.https://www.mdpi.com/2073-4352/12/11/1513green synthesisresistancezinc oxide nanoparticlescharacterization<i>Origanum majorana</i>synergism |
| spellingShingle | Mohamed Taha Yassin Abdulaziz Abdulrahman Al-Askar Khalid Maniah Fatimah O. Al-Otibi Green Synthesis of Zinc Oxide Nanocrystals Utilizing <i>Origanum majorana</i> Leaf Extract and Their Synergistic Patterns with Colistin against Multidrug-Resistant Bacterial Strains Crystals green synthesis resistance zinc oxide nanoparticles characterization <i>Origanum majorana</i> synergism |
| title | Green Synthesis of Zinc Oxide Nanocrystals Utilizing <i>Origanum majorana</i> Leaf Extract and Their Synergistic Patterns with Colistin against Multidrug-Resistant Bacterial Strains |
| title_full | Green Synthesis of Zinc Oxide Nanocrystals Utilizing <i>Origanum majorana</i> Leaf Extract and Their Synergistic Patterns with Colistin against Multidrug-Resistant Bacterial Strains |
| title_fullStr | Green Synthesis of Zinc Oxide Nanocrystals Utilizing <i>Origanum majorana</i> Leaf Extract and Their Synergistic Patterns with Colistin against Multidrug-Resistant Bacterial Strains |
| title_full_unstemmed | Green Synthesis of Zinc Oxide Nanocrystals Utilizing <i>Origanum majorana</i> Leaf Extract and Their Synergistic Patterns with Colistin against Multidrug-Resistant Bacterial Strains |
| title_short | Green Synthesis of Zinc Oxide Nanocrystals Utilizing <i>Origanum majorana</i> Leaf Extract and Their Synergistic Patterns with Colistin against Multidrug-Resistant Bacterial Strains |
| title_sort | green synthesis of zinc oxide nanocrystals utilizing i origanum majorana i leaf extract and their synergistic patterns with colistin against multidrug resistant bacterial strains |
| topic | green synthesis resistance zinc oxide nanoparticles characterization <i>Origanum majorana</i> synergism |
| url | https://www.mdpi.com/2073-4352/12/11/1513 |
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