Proteomic Analysis Reveals Salicylic Acid as a Pivotal Signal Molecule in Rice Response to Blast Disease Infection
Rice blast disease caused by a fungus, <i>Magnaporthe grisea</i>, is one of the most destructive diseases in rice production worldwide, and salicylic acid (SA) can efficiently decrease the damage of <i>M. grisea</i>. Here, we combined the 2-Dimensional-Liquid Chromatography a...
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MDPI AG
2022-06-01
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| Online Access: | https://www.mdpi.com/2223-7747/11/13/1702 |
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| author | Haiying Zhou Delight Hwarari Yunhui Zhang Xiaosong Mo Yuming Luo Hongyu Ma |
| author_facet | Haiying Zhou Delight Hwarari Yunhui Zhang Xiaosong Mo Yuming Luo Hongyu Ma |
| author_sort | Haiying Zhou |
| collection | DOAJ |
| description | Rice blast disease caused by a fungus, <i>Magnaporthe grisea</i>, is one of the most destructive diseases in rice production worldwide, and salicylic acid (SA) can efficiently decrease the damage of <i>M. grisea</i>. Here, we combined the 2-Dimensional-Liquid Chromatography and the Matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (2D-LC-MALDI-TOF-TOF MS) techniques to compare and identify differentially expressed labelled proteins by the isobaric tags for relative and absolute quantitation (iTRAQ) between the blast-resistant cultivar Minghui and the susceptible rice cultivar Nipponbare in response to blast fungus infection. The group samples were treated with salicylic acid and compared to control samples. A total of 139 DEPs from the two cultivars showed either more than a two-fold change or alternating regulation patterns. Protein functionality analysis also exhibited that these proteins are involved in a wide range of molecular functions including: energy-related activity (30%), signal transduction (11%), redox homeostasis (15%), amino acid and nitrogen metabolism (4%), carbohydrate metabolism (5%), protein folding and assembly (10%), protein hydrolysis (9%), protein synthesis (12%), and other unknown functions (4%). Specifically, we demonstrated that exogenous treatment with salicylic acid promoted recovery in both rice cultivars from <i>Magnaporthe grisea</i> infection by enhancing: the regulation of signal transduction, increasing energy conversion and production through the regulation of the glycolytic pathway, and other various biochemical processes. These findings may facilitate future studies of the molecular mechanisms of rice blast resistance. |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T12:40:10Z |
| publishDate | 2022-06-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-c2cb8affd0a4401c8a2caaae8ddffe2d2023-11-30T22:19:25ZengMDPI AGPlants2223-77472022-06-011113170210.3390/plants11131702Proteomic Analysis Reveals Salicylic Acid as a Pivotal Signal Molecule in Rice Response to Blast Disease InfectionHaiying Zhou0Delight Hwarari1Yunhui Zhang2Xiaosong Mo3Yuming Luo4Hongyu Ma5Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, Huaiyin Normal University, Huai’an 223300, ChinaCollege of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, ChinaCollege of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaJiangsu Grain and Oil Quality Monitoring Center, Nanjing 210031, ChinaJiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, Huaiyin Normal University, Huai’an 223300, ChinaCollege of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaRice blast disease caused by a fungus, <i>Magnaporthe grisea</i>, is one of the most destructive diseases in rice production worldwide, and salicylic acid (SA) can efficiently decrease the damage of <i>M. grisea</i>. Here, we combined the 2-Dimensional-Liquid Chromatography and the Matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (2D-LC-MALDI-TOF-TOF MS) techniques to compare and identify differentially expressed labelled proteins by the isobaric tags for relative and absolute quantitation (iTRAQ) between the blast-resistant cultivar Minghui and the susceptible rice cultivar Nipponbare in response to blast fungus infection. The group samples were treated with salicylic acid and compared to control samples. A total of 139 DEPs from the two cultivars showed either more than a two-fold change or alternating regulation patterns. Protein functionality analysis also exhibited that these proteins are involved in a wide range of molecular functions including: energy-related activity (30%), signal transduction (11%), redox homeostasis (15%), amino acid and nitrogen metabolism (4%), carbohydrate metabolism (5%), protein folding and assembly (10%), protein hydrolysis (9%), protein synthesis (12%), and other unknown functions (4%). Specifically, we demonstrated that exogenous treatment with salicylic acid promoted recovery in both rice cultivars from <i>Magnaporthe grisea</i> infection by enhancing: the regulation of signal transduction, increasing energy conversion and production through the regulation of the glycolytic pathway, and other various biochemical processes. These findings may facilitate future studies of the molecular mechanisms of rice blast resistance.https://www.mdpi.com/2223-7747/11/13/1702ricesalicylic acidblast diseaseproteomics |
| spellingShingle | Haiying Zhou Delight Hwarari Yunhui Zhang Xiaosong Mo Yuming Luo Hongyu Ma Proteomic Analysis Reveals Salicylic Acid as a Pivotal Signal Molecule in Rice Response to Blast Disease Infection Plants rice salicylic acid blast disease proteomics |
| title | Proteomic Analysis Reveals Salicylic Acid as a Pivotal Signal Molecule in Rice Response to Blast Disease Infection |
| title_full | Proteomic Analysis Reveals Salicylic Acid as a Pivotal Signal Molecule in Rice Response to Blast Disease Infection |
| title_fullStr | Proteomic Analysis Reveals Salicylic Acid as a Pivotal Signal Molecule in Rice Response to Blast Disease Infection |
| title_full_unstemmed | Proteomic Analysis Reveals Salicylic Acid as a Pivotal Signal Molecule in Rice Response to Blast Disease Infection |
| title_short | Proteomic Analysis Reveals Salicylic Acid as a Pivotal Signal Molecule in Rice Response to Blast Disease Infection |
| title_sort | proteomic analysis reveals salicylic acid as a pivotal signal molecule in rice response to blast disease infection |
| topic | rice salicylic acid blast disease proteomics |
| url | https://www.mdpi.com/2223-7747/11/13/1702 |
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