FGFBP1 as a potential biomarker predicting bacillus Calmette–Guérin response in bladder cancer
Accurate prediction of Bacillus Calmette–Guérin (BCG) response is essential to identify bladder cancer (BCa) patients most likely to respond sustainably, but no molecular marker predicting BCG response is available in clinical routine. Therefore, we first identified that fibroblast growth factor bin...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.954836/full |
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| author | Fei Li Henghui Zhang Yu Wang Zhihao Yao Kunfeng Xie Qixin Mo Qin Fan Lina Hou Fan Deng Wanlong Tan |
| author_facet | Fei Li Henghui Zhang Yu Wang Zhihao Yao Kunfeng Xie Qixin Mo Qin Fan Lina Hou Fan Deng Wanlong Tan |
| author_sort | Fei Li |
| collection | DOAJ |
| description | Accurate prediction of Bacillus Calmette–Guérin (BCG) response is essential to identify bladder cancer (BCa) patients most likely to respond sustainably, but no molecular marker predicting BCG response is available in clinical routine. Therefore, we first identified that fibroblast growth factor binding protein 1 (FGFBP1) was upregulated in failures of BCG therapy, and the increased FGFBP1 had a poor outcome for BCa patients in the E-MTAB-4321 and GSE19423 datasets. These different expression genes associated with FGFBP1 expression are mainly involved in neutrophil activation, neutrophil-mediated immunity, and tumor necrosis factor-mediated signal pathways in biological processes. A significant positive correlation was observed between FGFBP1 expression and regulatory T-cell (Treg) infiltration by the Spearman correlation test in the BCG cohort (r = 0.177) and The Cancer Genome Atlas (TCGA) cohort (r = 0.176), suggesting that FGFBP1 may influence the response of BCa patients to BCG immunotherapy through immune escape. Though FGFBP1 expression was positively correlated with the expressions of PD-L1, CTLA4, and PDCD1 in TCGA cohort, a strong association between FGFBP1 and PD-L1 expression was only detected in the BCG cohort (r = 0.750). Furthermore, elevated FGFBP1 was observed in BCa cell lines and tissues in comparison to corresponding normal controls by RT-qPCR, Western blotting, and immunohistochemical staining. Increased FGFBP1 was further detected in the failures than in the responders by immunohistochemical staining. Notably, FGFBP1 is positively associated with PD-L1 expression in BCa patients with BCG treatment. To sum up, FGFBP1 in BCa tissue could be identified as a promising biomarker for the accurate prediction of BCG response in BCa. |
| first_indexed | 2024-04-11T18:35:50Z |
| format | Article |
| id | doaj.art-c2cbe22f764a431f88ff4ad3f1aacab4 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-11T18:35:50Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Immunology |
| spelling | doaj.art-c2cbe22f764a431f88ff4ad3f1aacab42022-12-22T04:09:18ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-09-011310.3389/fimmu.2022.954836954836FGFBP1 as a potential biomarker predicting bacillus Calmette–Guérin response in bladder cancerFei Li0Henghui Zhang1Yu Wang2Zhihao Yao3Kunfeng Xie4Qixin Mo5Qin Fan6Lina Hou7Fan Deng8Wanlong Tan9Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaSchool of Traditional Chinese Medicine, Southern Medical University, Guangzhou, ChinaDepartment of Healthy Management, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Cell Biology, School of Basic Medical Science, Southern Medical University, Guangzhou, ChinaDepartment of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaAccurate prediction of Bacillus Calmette–Guérin (BCG) response is essential to identify bladder cancer (BCa) patients most likely to respond sustainably, but no molecular marker predicting BCG response is available in clinical routine. Therefore, we first identified that fibroblast growth factor binding protein 1 (FGFBP1) was upregulated in failures of BCG therapy, and the increased FGFBP1 had a poor outcome for BCa patients in the E-MTAB-4321 and GSE19423 datasets. These different expression genes associated with FGFBP1 expression are mainly involved in neutrophil activation, neutrophil-mediated immunity, and tumor necrosis factor-mediated signal pathways in biological processes. A significant positive correlation was observed between FGFBP1 expression and regulatory T-cell (Treg) infiltration by the Spearman correlation test in the BCG cohort (r = 0.177) and The Cancer Genome Atlas (TCGA) cohort (r = 0.176), suggesting that FGFBP1 may influence the response of BCa patients to BCG immunotherapy through immune escape. Though FGFBP1 expression was positively correlated with the expressions of PD-L1, CTLA4, and PDCD1 in TCGA cohort, a strong association between FGFBP1 and PD-L1 expression was only detected in the BCG cohort (r = 0.750). Furthermore, elevated FGFBP1 was observed in BCa cell lines and tissues in comparison to corresponding normal controls by RT-qPCR, Western blotting, and immunohistochemical staining. Increased FGFBP1 was further detected in the failures than in the responders by immunohistochemical staining. Notably, FGFBP1 is positively associated with PD-L1 expression in BCa patients with BCG treatment. To sum up, FGFBP1 in BCa tissue could be identified as a promising biomarker for the accurate prediction of BCG response in BCa.https://www.frontiersin.org/articles/10.3389/fimmu.2022.954836/fullbladder cancerbacillus Calmette–GuérinFGFBP1biomarkerPD-L1 |
| spellingShingle | Fei Li Henghui Zhang Yu Wang Zhihao Yao Kunfeng Xie Qixin Mo Qin Fan Lina Hou Fan Deng Wanlong Tan FGFBP1 as a potential biomarker predicting bacillus Calmette–Guérin response in bladder cancer Frontiers in Immunology bladder cancer bacillus Calmette–Guérin FGFBP1 biomarker PD-L1 |
| title | FGFBP1 as a potential biomarker predicting bacillus Calmette–Guérin response in bladder cancer |
| title_full | FGFBP1 as a potential biomarker predicting bacillus Calmette–Guérin response in bladder cancer |
| title_fullStr | FGFBP1 as a potential biomarker predicting bacillus Calmette–Guérin response in bladder cancer |
| title_full_unstemmed | FGFBP1 as a potential biomarker predicting bacillus Calmette–Guérin response in bladder cancer |
| title_short | FGFBP1 as a potential biomarker predicting bacillus Calmette–Guérin response in bladder cancer |
| title_sort | fgfbp1 as a potential biomarker predicting bacillus calmette guerin response in bladder cancer |
| topic | bladder cancer bacillus Calmette–Guérin FGFBP1 biomarker PD-L1 |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.954836/full |
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