Fibre-Optic Surface Plasmon Resonance Biosensor for Monoclonal Antibody Titer Quantification
An extraordinary optical transmission fibre-optic surface plasmon resonance biosensing platform was engineered to improve its portability and sensitivity, and was applied to monitor the concentrations of monoclonal antibodies (Mabs). By refining the fabricating procedure and changing the material of...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/11/10/383 |
| _version_ | 1797515175424688128 |
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| author | Thai Thao Ly Yinlan Ruan Bobo Du Peipei Jia Hu Zhang |
| author_facet | Thai Thao Ly Yinlan Ruan Bobo Du Peipei Jia Hu Zhang |
| author_sort | Thai Thao Ly |
| collection | DOAJ |
| description | An extraordinary optical transmission fibre-optic surface plasmon resonance biosensing platform was engineered to improve its portability and sensitivity, and was applied to monitor the concentrations of monoclonal antibodies (Mabs). By refining the fabricating procedure and changing the material of the flow cell and the components of the optical fibre, the biosensor is portable and robust to external interference. After the implementation of an effective template cleaning procedure and precise control during the fabrication process, a consistent sensitivity of 509 ± 5 nm per refractive index unit (nm/RIU) was achieved. The biosensor can detect the Mab with a limit of detection (LOD) of 0.44 µg/mL. The results show that the biosensor is a potential tool for the rapid quantification of Mab titers. The biosensor can be regenerated at least 10 times with 10 mM glycine (pH = 2.5), and consistent signal changes were obtained after regeneration. Moreover, the employment of a spacer arm SM(PEG)2, used for immobilising protein A onto the gold film, was demonstrated to be unable to improve the detecting sensitivity; thus, a simple procedure without the spacer arm could be used to prepare the protein A-based biosensor. Our results demonstrate that the fibre-optic surface plasmon resonance biosensor is competent for the real-time and on-line monitoring of antibody titers in the future as a process analytical technologies (PATs) tool for bioprocess developments and the manufacture of therapeutic antibodies. |
| first_indexed | 2024-03-10T06:41:48Z |
| format | Article |
| id | doaj.art-c2cd0b713fdd4fda86c995524a6490ac |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T06:41:48Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-c2cd0b713fdd4fda86c995524a6490ac2023-11-22T17:35:54ZengMDPI AGBiosensors2079-63742021-10-01111038310.3390/bios11100383Fibre-Optic Surface Plasmon Resonance Biosensor for Monoclonal Antibody Titer QuantificationThai Thao Ly0Yinlan Ruan1Bobo Du2Peipei Jia3Hu Zhang4School of Chemical Engineering and Advanced Materials, University of Adelaide, Adelaide 5005, AustraliaInstitute for Photonics and Advanced Sensing, University of Adelaide, Adelaide 5005, AustraliaKey Laboratory for Physical Electronics and Devices of the Ministry of Education, School of Electronic Science and Engineering, Xi’an Jiaotong University, Xi’an 710049, ChinaShenzhen Institute for Advanced Study, University of Electronic Science and Technology of China, Shenzhen 518000, ChinaKeck Graduate Institute, Claremont, CA 91711, USAAn extraordinary optical transmission fibre-optic surface plasmon resonance biosensing platform was engineered to improve its portability and sensitivity, and was applied to monitor the concentrations of monoclonal antibodies (Mabs). By refining the fabricating procedure and changing the material of the flow cell and the components of the optical fibre, the biosensor is portable and robust to external interference. After the implementation of an effective template cleaning procedure and precise control during the fabrication process, a consistent sensitivity of 509 ± 5 nm per refractive index unit (nm/RIU) was achieved. The biosensor can detect the Mab with a limit of detection (LOD) of 0.44 µg/mL. The results show that the biosensor is a potential tool for the rapid quantification of Mab titers. The biosensor can be regenerated at least 10 times with 10 mM glycine (pH = 2.5), and consistent signal changes were obtained after regeneration. Moreover, the employment of a spacer arm SM(PEG)2, used for immobilising protein A onto the gold film, was demonstrated to be unable to improve the detecting sensitivity; thus, a simple procedure without the spacer arm could be used to prepare the protein A-based biosensor. Our results demonstrate that the fibre-optic surface plasmon resonance biosensor is competent for the real-time and on-line monitoring of antibody titers in the future as a process analytical technologies (PATs) tool for bioprocess developments and the manufacture of therapeutic antibodies.https://www.mdpi.com/2079-6374/11/10/383antibody titeroptical fibreSPR biosensorprocess analytical technology |
| spellingShingle | Thai Thao Ly Yinlan Ruan Bobo Du Peipei Jia Hu Zhang Fibre-Optic Surface Plasmon Resonance Biosensor for Monoclonal Antibody Titer Quantification Biosensors antibody titer optical fibre SPR biosensor process analytical technology |
| title | Fibre-Optic Surface Plasmon Resonance Biosensor for Monoclonal Antibody Titer Quantification |
| title_full | Fibre-Optic Surface Plasmon Resonance Biosensor for Monoclonal Antibody Titer Quantification |
| title_fullStr | Fibre-Optic Surface Plasmon Resonance Biosensor for Monoclonal Antibody Titer Quantification |
| title_full_unstemmed | Fibre-Optic Surface Plasmon Resonance Biosensor for Monoclonal Antibody Titer Quantification |
| title_short | Fibre-Optic Surface Plasmon Resonance Biosensor for Monoclonal Antibody Titer Quantification |
| title_sort | fibre optic surface plasmon resonance biosensor for monoclonal antibody titer quantification |
| topic | antibody titer optical fibre SPR biosensor process analytical technology |
| url | https://www.mdpi.com/2079-6374/11/10/383 |
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