The Effect of Chromosome Arm 1BS on the Fertility of Alloplasmic Recombinant Lines in Bread Wheat with the <i>Hordeum vulgare</i> Cytoplasm
The genetic mechanisms of fertility restoration in alloplasmic bread wheat with the barley cytoplasm are poorly explored. The effect of the 1BS chromosome arm on the fertility of bread wheat with the <i>H. vulgare</i> cytoplasm was studied depending on the incompleteness/completeness of...
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MDPI AG
2021-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/6/1120 |
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| author | Nataliya V. Trubacheeva Mikhail G. Divashuk Anastasiya G. Chernook Igor A. Belan Ludmila P. Rosseeva Lidiya A. Pershina |
| author_facet | Nataliya V. Trubacheeva Mikhail G. Divashuk Anastasiya G. Chernook Igor A. Belan Ludmila P. Rosseeva Lidiya A. Pershina |
| author_sort | Nataliya V. Trubacheeva |
| collection | DOAJ |
| description | The genetic mechanisms of fertility restoration in alloplasmic bread wheat with the barley cytoplasm are poorly explored. The effect of the 1BS chromosome arm on the fertility of bread wheat with the <i>H. vulgare</i> cytoplasm was studied depending on the incompleteness/completeness of the cytonuclear compatibility. (i) Three self-fertile (SF) lines and one partially fertile (PF) line with an incomplete cytonuclear compatibility and (ii) four self-fertile (SF) lines with a complete cytonuclear compatibility were studied. For the lines in group (i), the heteroplasmy (simultaneous presence of barley and wheat copies) of the 18S/5S mitochondrial (mt) repeat was revealed as well as the barley-type homoplasmy of chloroplast simple sequence repeats (cpSSRs). In the lines in group (ii), the 18S/5S mt repeat and cpSSRs were found in the wheat-type homoplasmic state. In all of the lines, the 1BS chromosome arm was substituted for the 1RS arm. The F<sub>1</sub> plants of SF(i)-1BS × 1RS hybrids were fertile. The results of a segregation analysis in the F<sub>2</sub> plants of SF(i)-1BS × 1RS showed that 1BS carries a single dominant fertility restorer gene (<i>Rf</i>) of bread wheat with the <i>H. vulgare</i> cytoplasm. All of the F<sub>1</sub> plants of PF(i)-1BS × 1RS hybrids were sterile. A single dose of this restorer gene is not sufficient to restore fertility in this alloplasmic PF(i) line. All of the F<sub>1</sub> and F<sub>2</sub> plants of SF(ii)-1BS × 1RS hybrids were self-fertile. |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-10T10:50:08Z |
| publishDate | 2021-05-01 |
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| series | Plants |
| spelling | doaj.art-c2ce22492ffc41b4b58f62701b7baebf2023-11-21T22:20:58ZengMDPI AGPlants2223-77472021-05-01106112010.3390/plants10061120The Effect of Chromosome Arm 1BS on the Fertility of Alloplasmic Recombinant Lines in Bread Wheat with the <i>Hordeum vulgare</i> CytoplasmNataliya V. Trubacheeva0Mikhail G. Divashuk1Anastasiya G. Chernook2Igor A. Belan3Ludmila P. Rosseeva4Lidiya A. Pershina5Institute of Cytology and Genetics, SB RAS, Lavrentiev av., 10, 630090 Novosibirsk, RussiaKurchatov Genomics Center of ARRIAB, All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya Street, 42, 127550 Moscow, RussiaMoscow Timiryazev Agricultural Academy, Russian State Agrarian University, Timiryazevskaya Street, 49, 127550 Moscow, RussiaOmsk Agricultural Scientific Center, 644012 Omsk, RussiaOmsk Agricultural Scientific Center, 644012 Omsk, RussiaInstitute of Cytology and Genetics, SB RAS, Lavrentiev av., 10, 630090 Novosibirsk, RussiaThe genetic mechanisms of fertility restoration in alloplasmic bread wheat with the barley cytoplasm are poorly explored. The effect of the 1BS chromosome arm on the fertility of bread wheat with the <i>H. vulgare</i> cytoplasm was studied depending on the incompleteness/completeness of the cytonuclear compatibility. (i) Three self-fertile (SF) lines and one partially fertile (PF) line with an incomplete cytonuclear compatibility and (ii) four self-fertile (SF) lines with a complete cytonuclear compatibility were studied. For the lines in group (i), the heteroplasmy (simultaneous presence of barley and wheat copies) of the 18S/5S mitochondrial (mt) repeat was revealed as well as the barley-type homoplasmy of chloroplast simple sequence repeats (cpSSRs). In the lines in group (ii), the 18S/5S mt repeat and cpSSRs were found in the wheat-type homoplasmic state. In all of the lines, the 1BS chromosome arm was substituted for the 1RS arm. The F<sub>1</sub> plants of SF(i)-1BS × 1RS hybrids were fertile. The results of a segregation analysis in the F<sub>2</sub> plants of SF(i)-1BS × 1RS showed that 1BS carries a single dominant fertility restorer gene (<i>Rf</i>) of bread wheat with the <i>H. vulgare</i> cytoplasm. All of the F<sub>1</sub> plants of PF(i)-1BS × 1RS hybrids were sterile. A single dose of this restorer gene is not sufficient to restore fertility in this alloplasmic PF(i) line. All of the F<sub>1</sub> and F<sub>2</sub> plants of SF(ii)-1BS × 1RS hybrids were self-fertile.https://www.mdpi.com/2223-7747/10/6/1120alloplasmic lines (<i>H. vulgare</i>)-<i>T. aestivum</i>mitochondrial and chloroplast DNAcytonuclear compatibility1BS chromosome arm |
| spellingShingle | Nataliya V. Trubacheeva Mikhail G. Divashuk Anastasiya G. Chernook Igor A. Belan Ludmila P. Rosseeva Lidiya A. Pershina The Effect of Chromosome Arm 1BS on the Fertility of Alloplasmic Recombinant Lines in Bread Wheat with the <i>Hordeum vulgare</i> Cytoplasm Plants alloplasmic lines (<i>H. vulgare</i>)-<i>T. aestivum</i> mitochondrial and chloroplast DNA cytonuclear compatibility 1BS chromosome arm |
| title | The Effect of Chromosome Arm 1BS on the Fertility of Alloplasmic Recombinant Lines in Bread Wheat with the <i>Hordeum vulgare</i> Cytoplasm |
| title_full | The Effect of Chromosome Arm 1BS on the Fertility of Alloplasmic Recombinant Lines in Bread Wheat with the <i>Hordeum vulgare</i> Cytoplasm |
| title_fullStr | The Effect of Chromosome Arm 1BS on the Fertility of Alloplasmic Recombinant Lines in Bread Wheat with the <i>Hordeum vulgare</i> Cytoplasm |
| title_full_unstemmed | The Effect of Chromosome Arm 1BS on the Fertility of Alloplasmic Recombinant Lines in Bread Wheat with the <i>Hordeum vulgare</i> Cytoplasm |
| title_short | The Effect of Chromosome Arm 1BS on the Fertility of Alloplasmic Recombinant Lines in Bread Wheat with the <i>Hordeum vulgare</i> Cytoplasm |
| title_sort | effect of chromosome arm 1bs on the fertility of alloplasmic recombinant lines in bread wheat with the i hordeum vulgare i cytoplasm |
| topic | alloplasmic lines (<i>H. vulgare</i>)-<i>T. aestivum</i> mitochondrial and chloroplast DNA cytonuclear compatibility 1BS chromosome arm |
| url | https://www.mdpi.com/2223-7747/10/6/1120 |
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