Comparative analysis of the daily liver transcriptomes in wild nocturnal bats
Abstract Background Mammals rely on the circadian clock network to regulate daily systemic metabolism and physiological activities. The liver is an important peripheral organ in mammals, and it has a unique circadian rhythm regulation process. As the only mammals that can fly, bats have attracted mu...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-08-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-022-08823-y |
| _version_ | 1811215861103460352 |
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| author | Yujia Chu Jingjing Li Lei Feng Guoting Zhang Hui Wu Tinglei Jiang Hui Wang Jiang Feng |
| author_facet | Yujia Chu Jingjing Li Lei Feng Guoting Zhang Hui Wu Tinglei Jiang Hui Wang Jiang Feng |
| author_sort | Yujia Chu |
| collection | DOAJ |
| description | Abstract Background Mammals rely on the circadian clock network to regulate daily systemic metabolism and physiological activities. The liver is an important peripheral organ in mammals, and it has a unique circadian rhythm regulation process. As the only mammals that can fly, bats have attracted much research attention due to their nocturnal habits and life histories. However, few research reports exist concerning the circadian rhythms of bat liver gene expression and the relevant biological clock regulation mechanisms in the liver. Results In this study, the expression levels of liver genes of Asian particolored bats were comparatively analyzed using RNA-seq at four different time points across 24 h. A total of 996 genes were found to be rhythmic, accounting for 65% of the total number of expressed genes. The critical circadian rhythm genes Bmal1, Rev-erbα, Cry, and Ror in the liver exhibited different expression patterns throughout the day, and participated in physiological processes with rhythmic changes, including Th17 cell differentiation (ko04659), antigen processing and presentation (ko04612), the estrogen signaling pathway (ko04915), and insulin resistance (ko04931). In addition, previous studies have found that the peroxisome proliferator-activated receptor (PPAR) metabolic signaling pathway (ko03320) may play a vital role in the rhythmic regulation of the metabolic network. Conclusions This study is the first to demonstrate diurnal changes in bat liver gene expression and related physiological processes. The results have thus further enriched our understanding of bats’ biological clocks. |
| first_indexed | 2024-04-12T06:30:36Z |
| format | Article |
| id | doaj.art-c2cf7924400942eda82b2719f4b602df |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-12T06:30:36Z |
| publishDate | 2022-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-c2cf7924400942eda82b2719f4b602df2022-12-22T03:44:02ZengBMCBMC Genomics1471-21642022-08-0123111310.1186/s12864-022-08823-yComparative analysis of the daily liver transcriptomes in wild nocturnal batsYujia Chu0Jingjing Li1Lei Feng2Guoting Zhang3Hui Wu4Tinglei Jiang5Hui Wang6Jiang Feng7College of Life Science, Jilin Agricultural UniversityCollege of Life Science, Jilin Agricultural UniversityJilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, Northeast Normal UniversityCollege of Life Science, Jilin Agricultural UniversityCollege of Life Science, Jilin Agricultural UniversityJilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, Northeast Normal UniversityCollege of Life Science, Jilin Agricultural UniversityCollege of Life Science, Jilin Agricultural UniversityAbstract Background Mammals rely on the circadian clock network to regulate daily systemic metabolism and physiological activities. The liver is an important peripheral organ in mammals, and it has a unique circadian rhythm regulation process. As the only mammals that can fly, bats have attracted much research attention due to their nocturnal habits and life histories. However, few research reports exist concerning the circadian rhythms of bat liver gene expression and the relevant biological clock regulation mechanisms in the liver. Results In this study, the expression levels of liver genes of Asian particolored bats were comparatively analyzed using RNA-seq at four different time points across 24 h. A total of 996 genes were found to be rhythmic, accounting for 65% of the total number of expressed genes. The critical circadian rhythm genes Bmal1, Rev-erbα, Cry, and Ror in the liver exhibited different expression patterns throughout the day, and participated in physiological processes with rhythmic changes, including Th17 cell differentiation (ko04659), antigen processing and presentation (ko04612), the estrogen signaling pathway (ko04915), and insulin resistance (ko04931). In addition, previous studies have found that the peroxisome proliferator-activated receptor (PPAR) metabolic signaling pathway (ko03320) may play a vital role in the rhythmic regulation of the metabolic network. Conclusions This study is the first to demonstrate diurnal changes in bat liver gene expression and related physiological processes. The results have thus further enriched our understanding of bats’ biological clocks.https://doi.org/10.1186/s12864-022-08823-yBatCircadian rhythmLiverComparative transcriptome |
| spellingShingle | Yujia Chu Jingjing Li Lei Feng Guoting Zhang Hui Wu Tinglei Jiang Hui Wang Jiang Feng Comparative analysis of the daily liver transcriptomes in wild nocturnal bats BMC Genomics Bat Circadian rhythm Liver Comparative transcriptome |
| title | Comparative analysis of the daily liver transcriptomes in wild nocturnal bats |
| title_full | Comparative analysis of the daily liver transcriptomes in wild nocturnal bats |
| title_fullStr | Comparative analysis of the daily liver transcriptomes in wild nocturnal bats |
| title_full_unstemmed | Comparative analysis of the daily liver transcriptomes in wild nocturnal bats |
| title_short | Comparative analysis of the daily liver transcriptomes in wild nocturnal bats |
| title_sort | comparative analysis of the daily liver transcriptomes in wild nocturnal bats |
| topic | Bat Circadian rhythm Liver Comparative transcriptome |
| url | https://doi.org/10.1186/s12864-022-08823-y |
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