Comparative efficacy of different concentrations of egg yolk for cryopreservation of goat semen
Gaddi goats are important livestock species of Himachal Pradesh, India. The sensitivity to cryopreservation varies among different species as also between animals of same species. Ejaculates (180) from 11 adult Gaddi bucks aged between 1.1 to 4.5 years (2.16±0.36 years), weighing 31–57 kg (39.1±2.8...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2020-09-01
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| Series: | Indian Journal of Animal Sciences |
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| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/104197 |
| _version_ | 1828005909914189824 |
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| author | AMIT SHARMA PANKAJ SOOD JITENDRA KUMAR CHAUDHARY |
| author_facet | AMIT SHARMA PANKAJ SOOD JITENDRA KUMAR CHAUDHARY |
| author_sort | AMIT SHARMA |
| collection | DOAJ |
| description |
Gaddi goats are important livestock species of Himachal Pradesh, India. The sensitivity to cryopreservation varies among different species as also between animals of same species. Ejaculates (180) from 11 adult Gaddi bucks aged between 1.1 to 4.5 years (2.16±0.36 years), weighing 31–57 kg (39.1±2.82 kg) were collected using artificial vagina and selected on basis of standard quality parameters. The ejaculates were extended in Tris citrate egg yolk extender containing 6% Glycerol with varying concentrations of egg yolk (EY; 5, 10, 15 and 20%) to maintain a concentration of 150 × 106 sperms/straws. Filled and sealed straws were equilibrated at 5°C for 4 h followed by vapour freezing of straws for 7 min at 4 cm above the liquid nitrogen and finally plunged into liquid nitrogen. The representative straws from each ejaculates were thawed at 37°C for 30 sec, 24 h post incubation to compare the progressive motility, viability, morphological abnormalities and HOST reactive sperms in between different EY concentrations along with per cent change due to the processing. The data was analyzed using package R version 3.4.3. Post thaw progressive motility (35.18±0.87) and viability (45.26±1.32) was higher with least per cent change due to processing (52.03 and 40.12) in 10% EY than other EY concentrations. Absolute average values of morphological abnormalities, were least in 10% EY (7.93±0.28) than 20, 15 and 5% EY (11.42±0.67, 10.84±0.53 and 8.39±0.35), respectively. The absolute average values of HOST did not differ between 15, 10 and 5% (59.96±1.93, 52.48±1.43 and 59.07±2.18) EY, all of which were higher than 20% (42.57±4.20) EY concentrations. In conclusion, extender containing 10% EY was best with respect to progressive motility and viability for Gaddi goat semen cryopreservation.
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| first_indexed | 2024-04-10T07:48:02Z |
| format | Article |
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| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-04-10T07:48:02Z |
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| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-c2d049e7a81741e5b1d4e417d7f64d5d2023-02-23T10:24:35ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272020-09-0190410.56093/ijans.v90i4.104197Comparative efficacy of different concentrations of egg yolk for cryopreservation of goat semenAMIT SHARMA0PANKAJ SOOD1JITENDRA KUMAR CHAUDHARY2CSK Himachal Pradesh Krishi Vishvavidyalya, Palampur, Himachal Pradesh 176 062 IndiaCSK Himachal Pradesh Krishi Vishvavidyalya, Palampur, Himachal Pradesh 176 062 IndiaCSK Himachal Pradesh Krishi Vishvavidyalya, Palampur, Himachal Pradesh 176 062 India Gaddi goats are important livestock species of Himachal Pradesh, India. The sensitivity to cryopreservation varies among different species as also between animals of same species. Ejaculates (180) from 11 adult Gaddi bucks aged between 1.1 to 4.5 years (2.16±0.36 years), weighing 31–57 kg (39.1±2.82 kg) were collected using artificial vagina and selected on basis of standard quality parameters. The ejaculates were extended in Tris citrate egg yolk extender containing 6% Glycerol with varying concentrations of egg yolk (EY; 5, 10, 15 and 20%) to maintain a concentration of 150 × 106 sperms/straws. Filled and sealed straws were equilibrated at 5°C for 4 h followed by vapour freezing of straws for 7 min at 4 cm above the liquid nitrogen and finally plunged into liquid nitrogen. The representative straws from each ejaculates were thawed at 37°C for 30 sec, 24 h post incubation to compare the progressive motility, viability, morphological abnormalities and HOST reactive sperms in between different EY concentrations along with per cent change due to the processing. The data was analyzed using package R version 3.4.3. Post thaw progressive motility (35.18±0.87) and viability (45.26±1.32) was higher with least per cent change due to processing (52.03 and 40.12) in 10% EY than other EY concentrations. Absolute average values of morphological abnormalities, were least in 10% EY (7.93±0.28) than 20, 15 and 5% EY (11.42±0.67, 10.84±0.53 and 8.39±0.35), respectively. The absolute average values of HOST did not differ between 15, 10 and 5% (59.96±1.93, 52.48±1.43 and 59.07±2.18) EY, all of which were higher than 20% (42.57±4.20) EY concentrations. In conclusion, extender containing 10% EY was best with respect to progressive motility and viability for Gaddi goat semen cryopreservation. https://epubs.icar.org.in/index.php/IJAnS/article/view/104197Egg yolkExtenderGaddi goatsSemen cryopreservation |
| spellingShingle | AMIT SHARMA PANKAJ SOOD JITENDRA KUMAR CHAUDHARY Comparative efficacy of different concentrations of egg yolk for cryopreservation of goat semen Indian Journal of Animal Sciences Egg yolk Extender Gaddi goats Semen cryopreservation |
| title | Comparative efficacy of different concentrations of egg yolk for cryopreservation of goat semen |
| title_full | Comparative efficacy of different concentrations of egg yolk for cryopreservation of goat semen |
| title_fullStr | Comparative efficacy of different concentrations of egg yolk for cryopreservation of goat semen |
| title_full_unstemmed | Comparative efficacy of different concentrations of egg yolk for cryopreservation of goat semen |
| title_short | Comparative efficacy of different concentrations of egg yolk for cryopreservation of goat semen |
| title_sort | comparative efficacy of different concentrations of egg yolk for cryopreservation of goat semen |
| topic | Egg yolk Extender Gaddi goats Semen cryopreservation |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/104197 |
| work_keys_str_mv | AT amitsharma comparativeefficacyofdifferentconcentrationsofeggyolkforcryopreservationofgoatsemen AT pankajsood comparativeefficacyofdifferentconcentrationsofeggyolkforcryopreservationofgoatsemen AT jitendrakumarchaudhary comparativeefficacyofdifferentconcentrationsofeggyolkforcryopreservationofgoatsemen |