A Novel Blocking Enzyme-Linked Immunosorbent Assay Based on a Biotinylated Nanobody for the Rapid and Sensitive Clinical Detection of Classical Swine Fever Virus Antibodies
ABSTRACT Monoclonal and polyclonal antibodies are mostly used for the development of traditional enzyme-linked immunosorbent assays (ELISAs), but the use of certain conventional antibodies may be limited by their low yield, the difficulty of their isolation, and their high cost. Heavy-chain antibodi...
Main Authors: | Zhi Cao, Dehua Yin, Lihong Zhang, Shuai Ma, Ke Zhang, Ruimei Yang, Hu Shan, Zhihua Qin |
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Format: | Article |
Language: | English |
Published: |
American Society for Microbiology
2023-02-01
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Series: | Microbiology Spectrum |
Subjects: | |
Online Access: | https://journals.asm.org/doi/10.1128/spectrum.02996-22 |
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