A Novel Blocking Enzyme-Linked Immunosorbent Assay Based on a Biotinylated Nanobody for the Rapid and Sensitive Clinical Detection of Classical Swine Fever Virus Antibodies

A Novel Blocking Enzyme-Linked Immunosorbent Assay Based on a Biotinylated Nanobody for the Rapid and Sensitive Clinical Detection of Classical Swine Fever Virus Antibodies

ABSTRACT Monoclonal and polyclonal antibodies are mostly used for the development of traditional enzyme-linked immunosorbent assays (ELISAs), but the use of certain conventional antibodies may be limited by their low yield, the difficulty of their isolation, and their high cost. Heavy-chain antibodi...

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Bibliographic Details
Main Authors: Zhi Cao, Dehua Yin, Lihong Zhang, Shuai Ma, Ke Zhang, Ruimei Yang, Hu Shan, Zhihua Qin
Format: Article
Language:English
Published: American Society for Microbiology 2023-02-01
Series:Microbiology Spectrum
Subjects:
classical swine fever virus
E2 recombinant protein
nanobody
blocking ELISA
Online Access:https://journals.asm.org/doi/10.1128/spectrum.02996-22

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