Label-free sensitive detection of influenza virus using PZT discs with a synthetic sialylglycopolymer receptor layer
We describe rapid, label-free detection of Influenza A viruses using the first radial mode of oscillations of lead zirconate titanate (PZT) piezoelectric discs with a 2 mm radius and 100 µm thickness fabricated from a piezoelectric membrane. The discs are modified with a synthetic sialylglycopolymer...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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The Royal Society
2019-09-01
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| Series: | Royal Society Open Science |
| Subjects: | |
| Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.190255 |
| _version_ | 1818151348598734848 |
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| author | Alexander S. Erofeev Petr V. Gorelkin Dmitry V. Kolesov Gleb A. Kiselev Evgeniy V. Dubrovin Igor V. Yaminsky |
| author_facet | Alexander S. Erofeev Petr V. Gorelkin Dmitry V. Kolesov Gleb A. Kiselev Evgeniy V. Dubrovin Igor V. Yaminsky |
| author_sort | Alexander S. Erofeev |
| collection | DOAJ |
| description | We describe rapid, label-free detection of Influenza A viruses using the first radial mode of oscillations of lead zirconate titanate (PZT) piezoelectric discs with a 2 mm radius and 100 µm thickness fabricated from a piezoelectric membrane. The discs are modified with a synthetic sialylglycopolymer receptor layer, and the coated discs are inserted in a flowing virus suspension. Label-free detection of the virus is achieved by monitoring the disc radial mode resonance frequency shift. Piezo transducers with sialylglycopolymer sensor layers exhibited a long lifetime, a high sensitivity and the possibility of regeneration. We demonstrate positive, label-free detection of Influenza A viruses at concentrations below 105 virus particles per millilitre. We show that label-free, selective, sensitive detection of influenza viruses by home appliances is possible in principle. |
| first_indexed | 2024-12-11T13:37:24Z |
| format | Article |
| id | doaj.art-c2e0fad7a9a54e45b11a46d80edaec0b |
| institution | Directory Open Access Journal |
| issn | 2054-5703 |
| language | English |
| last_indexed | 2024-12-11T13:37:24Z |
| publishDate | 2019-09-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Royal Society Open Science |
| spelling | doaj.art-c2e0fad7a9a54e45b11a46d80edaec0b2022-12-22T01:04:58ZengThe Royal SocietyRoyal Society Open Science2054-57032019-09-016910.1098/rsos.190255190255Label-free sensitive detection of influenza virus using PZT discs with a synthetic sialylglycopolymer receptor layerAlexander S. ErofeevPetr V. GorelkinDmitry V. KolesovGleb A. KiselevEvgeniy V. DubrovinIgor V. YaminskyWe describe rapid, label-free detection of Influenza A viruses using the first radial mode of oscillations of lead zirconate titanate (PZT) piezoelectric discs with a 2 mm radius and 100 µm thickness fabricated from a piezoelectric membrane. The discs are modified with a synthetic sialylglycopolymer receptor layer, and the coated discs are inserted in a flowing virus suspension. Label-free detection of the virus is achieved by monitoring the disc radial mode resonance frequency shift. Piezo transducers with sialylglycopolymer sensor layers exhibited a long lifetime, a high sensitivity and the possibility of regeneration. We demonstrate positive, label-free detection of Influenza A viruses at concentrations below 105 virus particles per millilitre. We show that label-free, selective, sensitive detection of influenza viruses by home appliances is possible in principle.https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.190255microelectromechanical systemsbiosensorvirus detectionsialylglycopolymerinfluenza |
| spellingShingle | Alexander S. Erofeev Petr V. Gorelkin Dmitry V. Kolesov Gleb A. Kiselev Evgeniy V. Dubrovin Igor V. Yaminsky Label-free sensitive detection of influenza virus using PZT discs with a synthetic sialylglycopolymer receptor layer Royal Society Open Science microelectromechanical systems biosensor virus detection sialylglycopolymer influenza |
| title | Label-free sensitive detection of influenza virus using PZT discs with a synthetic sialylglycopolymer receptor layer |
| title_full | Label-free sensitive detection of influenza virus using PZT discs with a synthetic sialylglycopolymer receptor layer |
| title_fullStr | Label-free sensitive detection of influenza virus using PZT discs with a synthetic sialylglycopolymer receptor layer |
| title_full_unstemmed | Label-free sensitive detection of influenza virus using PZT discs with a synthetic sialylglycopolymer receptor layer |
| title_short | Label-free sensitive detection of influenza virus using PZT discs with a synthetic sialylglycopolymer receptor layer |
| title_sort | label free sensitive detection of influenza virus using pzt discs with a synthetic sialylglycopolymer receptor layer |
| topic | microelectromechanical systems biosensor virus detection sialylglycopolymer influenza |
| url | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.190255 |
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