In Vitro Antibiofilm Activity of Resveratrol against <i>Aeromonas hydrophila</i>
<i>Aeromonas hydrophila</i> is a Gram-negative bacterium that widely exists in various aquatic environments and causes septicemia in fish and humans. Resveratrol, a natural polyterpenoid product, has potential chemo-preventive and antibacterial properties. In this study, we investigated...
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MDPI AG
2023-03-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/4/686 |
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| author | Ting Qin Kai Chen Bingwen Xi Liangkun Pan Jun Xie Liushen Lu Kai Liu |
| author_facet | Ting Qin Kai Chen Bingwen Xi Liangkun Pan Jun Xie Liushen Lu Kai Liu |
| author_sort | Ting Qin |
| collection | DOAJ |
| description | <i>Aeromonas hydrophila</i> is a Gram-negative bacterium that widely exists in various aquatic environments and causes septicemia in fish and humans. Resveratrol, a natural polyterpenoid product, has potential chemo-preventive and antibacterial properties. In this study, we investigated the effect of resveratrol on <i>A. hydrophila</i> biofilm formation and motility. The results demonstrated that resveratrol, at sub-MIC levels, can significantly inhibit the biofilm formation of <i>A. hydrophila</i>, and the biofilm was decreased with increasing concentrations. The motility assay showed that resveratrol could diminish the swimming and swarming motility of <i>A. hydrophila</i>. Transcriptome analyses (RNA-seq) showed that <i>A. hydrophila</i> treated with 50 and 100 μg/mL resveratrol, respectively, presented 230 and 308 differentially expressed genes (DEGs), including 90 or 130 upregulated genes and 130 or 178 downregulated genes. Among them, genes related to flagellar, type IV pilus and chemotaxis were significantly repressed. In addition, mRNA of virulence factors OmpA, extracellular proteases, lipases and T6SS were dramatically suppressed. Further analysis revealed that the major DEGs involved in flagellar assembly and bacterial chemotaxis pathways could be regulated by cyclic-di-guanosine monophosphate (c-di-GMP)- and LysR-Type transcriptional regulator (LTTR)-dependent quorum sensing (QS) systems. Overall, our results indicate that resveratrol can inhibit <i>A. hydrophila</i> biofilm formation by disturbing motility and QS systems, and can be used as a promising candidate drug against motile Aeromonad septicemia. |
| first_indexed | 2024-03-11T05:19:18Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-11T05:19:18Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-c2e486b4e9f24b698b0aa08ca12257602023-11-17T18:02:32ZengMDPI AGAntibiotics2079-63822023-03-0112468610.3390/antibiotics12040686In Vitro Antibiofilm Activity of Resveratrol against <i>Aeromonas hydrophila</i>Ting Qin0Kai Chen1Bingwen Xi2Liangkun Pan3Jun Xie4Liushen Lu5Kai Liu6Key Laboratory of Aquatic Animal Nutrition and Health, Freshwater Fisheries Research Center, Chinese Academy of Fishery Science, Wuxi 214081, ChinaKey Laboratory of Aquatic Animal Nutrition and Health, Freshwater Fisheries Research Center, Chinese Academy of Fishery Science, Wuxi 214081, ChinaKey Laboratory of Aquatic Animal Nutrition and Health, Freshwater Fisheries Research Center, Chinese Academy of Fishery Science, Wuxi 214081, ChinaKey Laboratory of Aquatic Animal Nutrition and Health, Freshwater Fisheries Research Center, Chinese Academy of Fishery Science, Wuxi 214081, ChinaKey Laboratory of Aquatic Animal Nutrition and Health, Freshwater Fisheries Research Center, Chinese Academy of Fishery Science, Wuxi 214081, ChinaKey Laboratory of Aquatic Animal Nutrition and Health, Freshwater Fisheries Research Center, Chinese Academy of Fishery Science, Wuxi 214081, ChinaKey Laboratory of Aquatic Animal Nutrition and Health, Freshwater Fisheries Research Center, Chinese Academy of Fishery Science, Wuxi 214081, China<i>Aeromonas hydrophila</i> is a Gram-negative bacterium that widely exists in various aquatic environments and causes septicemia in fish and humans. Resveratrol, a natural polyterpenoid product, has potential chemo-preventive and antibacterial properties. In this study, we investigated the effect of resveratrol on <i>A. hydrophila</i> biofilm formation and motility. The results demonstrated that resveratrol, at sub-MIC levels, can significantly inhibit the biofilm formation of <i>A. hydrophila</i>, and the biofilm was decreased with increasing concentrations. The motility assay showed that resveratrol could diminish the swimming and swarming motility of <i>A. hydrophila</i>. Transcriptome analyses (RNA-seq) showed that <i>A. hydrophila</i> treated with 50 and 100 μg/mL resveratrol, respectively, presented 230 and 308 differentially expressed genes (DEGs), including 90 or 130 upregulated genes and 130 or 178 downregulated genes. Among them, genes related to flagellar, type IV pilus and chemotaxis were significantly repressed. In addition, mRNA of virulence factors OmpA, extracellular proteases, lipases and T6SS were dramatically suppressed. Further analysis revealed that the major DEGs involved in flagellar assembly and bacterial chemotaxis pathways could be regulated by cyclic-di-guanosine monophosphate (c-di-GMP)- and LysR-Type transcriptional regulator (LTTR)-dependent quorum sensing (QS) systems. Overall, our results indicate that resveratrol can inhibit <i>A. hydrophila</i> biofilm formation by disturbing motility and QS systems, and can be used as a promising candidate drug against motile Aeromonad septicemia.https://www.mdpi.com/2079-6382/12/4/686resveratrol<i>Aeromonas hydrophila</i>biofilm formationmotilityRNA-seqquorum sensing |
| spellingShingle | Ting Qin Kai Chen Bingwen Xi Liangkun Pan Jun Xie Liushen Lu Kai Liu In Vitro Antibiofilm Activity of Resveratrol against <i>Aeromonas hydrophila</i> Antibiotics resveratrol <i>Aeromonas hydrophila</i> biofilm formation motility RNA-seq quorum sensing |
| title | In Vitro Antibiofilm Activity of Resveratrol against <i>Aeromonas hydrophila</i> |
| title_full | In Vitro Antibiofilm Activity of Resveratrol against <i>Aeromonas hydrophila</i> |
| title_fullStr | In Vitro Antibiofilm Activity of Resveratrol against <i>Aeromonas hydrophila</i> |
| title_full_unstemmed | In Vitro Antibiofilm Activity of Resveratrol against <i>Aeromonas hydrophila</i> |
| title_short | In Vitro Antibiofilm Activity of Resveratrol against <i>Aeromonas hydrophila</i> |
| title_sort | in vitro antibiofilm activity of resveratrol against i aeromonas hydrophila i |
| topic | resveratrol <i>Aeromonas hydrophila</i> biofilm formation motility RNA-seq quorum sensing |
| url | https://www.mdpi.com/2079-6382/12/4/686 |
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