Transcriptome Analysis Reveals the Molecular Response to Salinity Challenge in Larvae of the Giant Freshwater Prawn Macrobrachium rosenbergii
Salinity is a crucial factor influencing the growth, development, immunity, and reproduction of aquatic organisms; however, little is known about the molecular mechanism of the response to salinity challenge in larvae of the giant freshwater prawn Macrobrachium rosenbergii. Herein, larvae cultured i...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2022.885035/full |
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| author | Yakun Wang Jie Wei Jie Wei Kunhao Hong Nan Zhou Nan Zhou Xiaoli Liu Xiaoyou Hong Wei Li Jian Zhao Chen Chen Liang Wu Lingyun Yu Xinping Zhu |
| author_facet | Yakun Wang Jie Wei Jie Wei Kunhao Hong Nan Zhou Nan Zhou Xiaoli Liu Xiaoyou Hong Wei Li Jian Zhao Chen Chen Liang Wu Lingyun Yu Xinping Zhu |
| author_sort | Yakun Wang |
| collection | DOAJ |
| description | Salinity is a crucial factor influencing the growth, development, immunity, and reproduction of aquatic organisms; however, little is known about the molecular mechanism of the response to salinity challenge in larvae of the giant freshwater prawn Macrobrachium rosenbergii. Herein, larvae cultured in three treatment groups with salinities of 10, 13, and 16‰ (S10, S13, and S16) were collected, and then transcriptome analysis was conducted by RNA-seq. A total of 6,473, 3,830 and 3,584 differentially expressed genes (DEGs) were identified in the S10 vs. S13 comparison, S10 vs. S16 comparison and S13 vs. S16 comparison, respectively. These genes are involved in osmoregulation, energy metabolism, molting, and the immune response. qPCR analysis was used to detect the expression patterns of 16 DEGs to verify the accuracy of the transcriptome data. Protein–protein interaction (PPI) analysis for DEGs and microsatellite marker screening were also conducted to reveal the molecular mechanism of salinity regulation. Together, our results will provide insight into the molecular genetic basis of adaptation to salinity challenge for larvae of M. rosenbergii. |
| first_indexed | 2024-04-13T07:54:00Z |
| format | Article |
| id | doaj.art-c2e6c9cd0dc5405092a9e85893c6b721 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-13T07:54:00Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-c2e6c9cd0dc5405092a9e85893c6b7212022-12-22T02:55:28ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2022-04-011310.3389/fphys.2022.885035885035Transcriptome Analysis Reveals the Molecular Response to Salinity Challenge in Larvae of the Giant Freshwater Prawn Macrobrachium rosenbergiiYakun Wang0Jie Wei1Jie Wei2Kunhao Hong3Nan Zhou4Nan Zhou5Xiaoli Liu6Xiaoyou Hong7Wei Li8Jian Zhao9Chen Chen10Liang Wu11Lingyun Yu12Xinping Zhu13Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaKey Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai, ChinaKey Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaKey Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaSchool of Fishery, Zhejiang Ocean University, Zhoushan, ChinaKey Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaKey Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaKey Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaKey Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaKey Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaSisal and Sisal Products Quality Supervision, Inspection and Testing Center, Ministry of Agriculture and Rural Affairs, Zhanjiang, ChinaKey Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaKey Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaSalinity is a crucial factor influencing the growth, development, immunity, and reproduction of aquatic organisms; however, little is known about the molecular mechanism of the response to salinity challenge in larvae of the giant freshwater prawn Macrobrachium rosenbergii. Herein, larvae cultured in three treatment groups with salinities of 10, 13, and 16‰ (S10, S13, and S16) were collected, and then transcriptome analysis was conducted by RNA-seq. A total of 6,473, 3,830 and 3,584 differentially expressed genes (DEGs) were identified in the S10 vs. S13 comparison, S10 vs. S16 comparison and S13 vs. S16 comparison, respectively. These genes are involved in osmoregulation, energy metabolism, molting, and the immune response. qPCR analysis was used to detect the expression patterns of 16 DEGs to verify the accuracy of the transcriptome data. Protein–protein interaction (PPI) analysis for DEGs and microsatellite marker screening were also conducted to reveal the molecular mechanism of salinity regulation. Together, our results will provide insight into the molecular genetic basis of adaptation to salinity challenge for larvae of M. rosenbergii.https://www.frontiersin.org/articles/10.3389/fphys.2022.885035/fullsalinity challengemolecular responsetranscriptome analysisRNAseqMacrobrachium rosenbergii |
| spellingShingle | Yakun Wang Jie Wei Jie Wei Kunhao Hong Nan Zhou Nan Zhou Xiaoli Liu Xiaoyou Hong Wei Li Jian Zhao Chen Chen Liang Wu Lingyun Yu Xinping Zhu Transcriptome Analysis Reveals the Molecular Response to Salinity Challenge in Larvae of the Giant Freshwater Prawn Macrobrachium rosenbergii Frontiers in Physiology salinity challenge molecular response transcriptome analysis RNAseq Macrobrachium rosenbergii |
| title | Transcriptome Analysis Reveals the Molecular Response to Salinity Challenge in Larvae of the Giant Freshwater Prawn Macrobrachium rosenbergii |
| title_full | Transcriptome Analysis Reveals the Molecular Response to Salinity Challenge in Larvae of the Giant Freshwater Prawn Macrobrachium rosenbergii |
| title_fullStr | Transcriptome Analysis Reveals the Molecular Response to Salinity Challenge in Larvae of the Giant Freshwater Prawn Macrobrachium rosenbergii |
| title_full_unstemmed | Transcriptome Analysis Reveals the Molecular Response to Salinity Challenge in Larvae of the Giant Freshwater Prawn Macrobrachium rosenbergii |
| title_short | Transcriptome Analysis Reveals the Molecular Response to Salinity Challenge in Larvae of the Giant Freshwater Prawn Macrobrachium rosenbergii |
| title_sort | transcriptome analysis reveals the molecular response to salinity challenge in larvae of the giant freshwater prawn macrobrachium rosenbergii |
| topic | salinity challenge molecular response transcriptome analysis RNAseq Macrobrachium rosenbergii |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2022.885035/full |
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