Plasma Ephrin-A1 level in a cohort of diabetic retinopathy patients
Abstract Background To determine plasma ephrin-A1 and VEGF165 levels in a cohort of diabetic retinopathy patients. Methods Plasma ephrin-A1 and VEGF165 levels in fifty-five subjects including 19 individuals without diabetes (non-DM), 16 patients with diabetes (DM) but without diabetic retinopathy, a...
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| Language: | English |
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BMC
2020-08-01
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| Series: | BMC Ophthalmology |
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| Online Access: | http://link.springer.com/article/10.1186/s12886-020-01580-0 |
| _version_ | 1818306218884595712 |
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| author | Danna Mao Ying Hu Qi Bao Kewei Wu Yaoding Zheng Yukun Yang Bo Lei Ying Jiang |
| author_facet | Danna Mao Ying Hu Qi Bao Kewei Wu Yaoding Zheng Yukun Yang Bo Lei Ying Jiang |
| author_sort | Danna Mao |
| collection | DOAJ |
| description | Abstract Background To determine plasma ephrin-A1 and VEGF165 levels in a cohort of diabetic retinopathy patients. Methods Plasma ephrin-A1 and VEGF165 levels in fifty-five subjects including 19 individuals without diabetes (non-DM), 16 patients with diabetes (DM) but without diabetic retinopathy, and 20 patients with diabetic retinopathy (DR), were determined by ELISA. Serum creatinine, total cholesterol, fasting blood glucose and HbA1c were also measured. One-way ANOVA, Kruskal-Wallis Test, Mann-Whitney U Test corrected by Bonferroni, Pearson Correlation Analysis and Spearman Correlation Coefficient Analysis were used for data analysis. Results Ephrin-A1 expression could be detected in human plasma with an average of 1.52 ± 0.43 (mean ± SEM) ng/ml. In DR subjects, the plasma ephrin-A1concentration was 3.63 ± 4.63 ng/ml, which was significantly higher than that of the other two groups (non-DM: 0.27 ± 0.13 ng/ml, DM: 0.35 ± 0.34 ng/ml). The expression of VEGF165 in human plasma was 34.00 ± 42.55 pg/ml, with no statistical difference among the three groups. There was no correlation between ephrin-A1 and VEGF165 in human plasma, but there was a correlation between plasma ephrin-A1 and duration of diabetes. Conclusions Plasma ephrin-A1 was highly expressed in patients with diabetic retinopathy, and there was no difference of plasma VEGF165 expression in patients with diabetic retinopathy compared to the other two groups, suggesting that changes of plasma ephrin-A1 may be a more sensitive biomarker than plasma VEGF165 in detecting diabetic retinopathy. |
| first_indexed | 2024-12-13T06:39:00Z |
| format | Article |
| id | doaj.art-c2e6f3b6d3394580aa522dbc99f38fce |
| institution | Directory Open Access Journal |
| issn | 1471-2415 |
| language | English |
| last_indexed | 2024-12-13T06:39:00Z |
| publishDate | 2020-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Ophthalmology |
| spelling | doaj.art-c2e6f3b6d3394580aa522dbc99f38fce2022-12-21T23:56:28ZengBMCBMC Ophthalmology1471-24152020-08-012011510.1186/s12886-020-01580-0Plasma Ephrin-A1 level in a cohort of diabetic retinopathy patientsDanna Mao0Ying Hu1Qi Bao2Kewei Wu3Yaoding Zheng4Yukun Yang5Bo Lei6Ying Jiang7Department of Ophthalmology, Medical community of Fenghua Hospital of Traditional Chinese MedicineDepartment of Ophthalmology, Medical community of Fenghua Hospital of Traditional Chinese MedicineDepartment of Clinical Laboratory, Medical community of Fenghua Hospital of Traditional Chinese MedicineDepartment of Ophthalmology, Medical community of Fenghua Hospital of Traditional Chinese MedicineDepartment of Ophthalmology, Medical community of Fenghua Hospital of Traditional Chinese MedicineDepartment of Ophthalmology, Medical community of Fenghua Hospital of Traditional Chinese MedicinePeople’s Hospital of Zhengzhou University, Henan Provincial People’s Hospital, Henan Eye Institute, Henan Eye InstituteDepartment of Ophthalmology, Medical community of Fenghua Hospital of Traditional Chinese MedicineAbstract Background To determine plasma ephrin-A1 and VEGF165 levels in a cohort of diabetic retinopathy patients. Methods Plasma ephrin-A1 and VEGF165 levels in fifty-five subjects including 19 individuals without diabetes (non-DM), 16 patients with diabetes (DM) but without diabetic retinopathy, and 20 patients with diabetic retinopathy (DR), were determined by ELISA. Serum creatinine, total cholesterol, fasting blood glucose and HbA1c were also measured. One-way ANOVA, Kruskal-Wallis Test, Mann-Whitney U Test corrected by Bonferroni, Pearson Correlation Analysis and Spearman Correlation Coefficient Analysis were used for data analysis. Results Ephrin-A1 expression could be detected in human plasma with an average of 1.52 ± 0.43 (mean ± SEM) ng/ml. In DR subjects, the plasma ephrin-A1concentration was 3.63 ± 4.63 ng/ml, which was significantly higher than that of the other two groups (non-DM: 0.27 ± 0.13 ng/ml, DM: 0.35 ± 0.34 ng/ml). The expression of VEGF165 in human plasma was 34.00 ± 42.55 pg/ml, with no statistical difference among the three groups. There was no correlation between ephrin-A1 and VEGF165 in human plasma, but there was a correlation between plasma ephrin-A1 and duration of diabetes. Conclusions Plasma ephrin-A1 was highly expressed in patients with diabetic retinopathy, and there was no difference of plasma VEGF165 expression in patients with diabetic retinopathy compared to the other two groups, suggesting that changes of plasma ephrin-A1 may be a more sensitive biomarker than plasma VEGF165 in detecting diabetic retinopathy.http://link.springer.com/article/10.1186/s12886-020-01580-0Ephrin-A1VEGF165PlasmaDiabetic retinopathyELISA |
| spellingShingle | Danna Mao Ying Hu Qi Bao Kewei Wu Yaoding Zheng Yukun Yang Bo Lei Ying Jiang Plasma Ephrin-A1 level in a cohort of diabetic retinopathy patients BMC Ophthalmology Ephrin-A1 VEGF165 Plasma Diabetic retinopathy ELISA |
| title | Plasma Ephrin-A1 level in a cohort of diabetic retinopathy patients |
| title_full | Plasma Ephrin-A1 level in a cohort of diabetic retinopathy patients |
| title_fullStr | Plasma Ephrin-A1 level in a cohort of diabetic retinopathy patients |
| title_full_unstemmed | Plasma Ephrin-A1 level in a cohort of diabetic retinopathy patients |
| title_short | Plasma Ephrin-A1 level in a cohort of diabetic retinopathy patients |
| title_sort | plasma ephrin a1 level in a cohort of diabetic retinopathy patients |
| topic | Ephrin-A1 VEGF165 Plasma Diabetic retinopathy ELISA |
| url | http://link.springer.com/article/10.1186/s12886-020-01580-0 |
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