ACTIVITY OF SUCROSE DISSIMILATING ENZYMES IN EARLY ONTOGENY IN DIFFERENT FORMS OF SILVER BIRCH
The distribution of interchangeable sucrose-cleaving enzymes’ activities in sink organs (stem, root) of silver birch (Betula pendula var. pendula) and Karelian birch (B. pendula var. carelica) seedlings was investigated. It is shown that the dominant sink organ in 1.5‑month-old seedlings is the root...
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| Format: | Article |
| Language: | English |
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Karelian Research Centre of the Russian Academy of Sciences
2016-11-01
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| Series: | Transactions of the Karelian Research Centre of the Russian Academy of Sciences |
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| Online Access: | http://journals.krc.karelia.ru/index.php/biology/article/view/461 |
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| author | Yulia Moshchenskaya Natalia Galibina Ksenia Nikerova Ludmila Novitskaya |
| author_facet | Yulia Moshchenskaya Natalia Galibina Ksenia Nikerova Ludmila Novitskaya |
| author_sort | Yulia Moshchenskaya |
| collection | DOAJ |
| description | The distribution of interchangeable sucrose-cleaving enzymes’ activities in sink organs (stem, root) of silver birch (Betula pendula var. pendula) and Karelian birch (B. pendula var. carelica) seedlings was investigated. It is shown that the dominant sink organ in 1.5‑month-old seedlings is the root, and sucrose cleavage is mainly performed by sucrose synthase. Early in the ontogeny sucrose metabolism in Karelian birch sink organs is more intensive compared to silver birch. As confirmed by the high activity of sucrosecleaving enzymes in the stem, the bulk of metabolites in 5‑month-old silver birch plants<br />were spent on the formation of the stem’s structure. We observed high activity of sucrose synthase in the stem of 5‑month-old silver birch seedlings and high activity cell-wall invertase in Karelian birch. Thus, biochemical and molecular differences between silver birch and Karelian birch originate from the early stages of the plants’ ontogeny, before<br />the traits of abnormal wood structure become visible in Karelian birch. The effect nitrogen availability on the activity of alternative sucrose-cleaving enzymes is shown. Cultivation of<br />experimental plants in nitrogen-deficient media suppressed sucrose metabolism in sink organs: through reduced activity of sucrose synthase in silver birch, and through reduced<br />activity of acid invertase in Karelian birch. This means that low nitrogen availability to silver birch seedlings resulted in inhibition of xylogenesis, and all the plant’s resources were utilized to support root biomass, whereas in Karelian birch it caused a reduction in sucrose cleavage in the apoplast, thus leading to an increase in the amount of parenchyma cells. |
| first_indexed | 2024-04-12T03:48:57Z |
| format | Article |
| id | doaj.art-c2e721aadbeb4ba88dc84ff595d31f82 |
| institution | Directory Open Access Journal |
| issn | 1997-3217 2312-4504 |
| language | English |
| last_indexed | 2024-04-12T03:48:57Z |
| publishDate | 2016-11-01 |
| publisher | Karelian Research Centre of the Russian Academy of Sciences |
| record_format | Article |
| series | Transactions of the Karelian Research Centre of the Russian Academy of Sciences |
| spelling | doaj.art-c2e721aadbeb4ba88dc84ff595d31f822022-12-22T03:49:03ZengKarelian Research Centre of the Russian Academy of SciencesTransactions of the Karelian Research Centre of the Russian Academy of Sciences1997-32172312-45042016-11-0111788710.17076/eb461322ACTIVITY OF SUCROSE DISSIMILATING ENZYMES IN EARLY ONTOGENY IN DIFFERENT FORMS OF SILVER BIRCHYulia Moshchenskaya0Natalia Galibina1Ksenia Nikerova2Ludmila Novitskaya3Forest Research Institute, Karelian Research Centre, Russian Academy of SciencesForest Research Institute, Karelian Research Centre, Russian Academy of SciencesForest Research Institute, Karelian Research Centre, Russian Academy of SciencesForest Research Institute, Karelian Research Centre, Russian Academy of SciencesThe distribution of interchangeable sucrose-cleaving enzymes’ activities in sink organs (stem, root) of silver birch (Betula pendula var. pendula) and Karelian birch (B. pendula var. carelica) seedlings was investigated. It is shown that the dominant sink organ in 1.5‑month-old seedlings is the root, and sucrose cleavage is mainly performed by sucrose synthase. Early in the ontogeny sucrose metabolism in Karelian birch sink organs is more intensive compared to silver birch. As confirmed by the high activity of sucrosecleaving enzymes in the stem, the bulk of metabolites in 5‑month-old silver birch plants<br />were spent on the formation of the stem’s structure. We observed high activity of sucrose synthase in the stem of 5‑month-old silver birch seedlings and high activity cell-wall invertase in Karelian birch. Thus, biochemical and molecular differences between silver birch and Karelian birch originate from the early stages of the plants’ ontogeny, before<br />the traits of abnormal wood structure become visible in Karelian birch. The effect nitrogen availability on the activity of alternative sucrose-cleaving enzymes is shown. Cultivation of<br />experimental plants in nitrogen-deficient media suppressed sucrose metabolism in sink organs: through reduced activity of sucrose synthase in silver birch, and through reduced<br />activity of acid invertase in Karelian birch. This means that low nitrogen availability to silver birch seedlings resulted in inhibition of xylogenesis, and all the plant’s resources were utilized to support root biomass, whereas in Karelian birch it caused a reduction in sucrose cleavage in the apoplast, thus leading to an increase in the amount of parenchyma cells.http://journals.krc.karelia.ru/index.php/biology/article/view/461betula pendula rothsucrose synthaseapoplastic invertasevacuolar invertasecytosolic invertase |
| spellingShingle | Yulia Moshchenskaya Natalia Galibina Ksenia Nikerova Ludmila Novitskaya ACTIVITY OF SUCROSE DISSIMILATING ENZYMES IN EARLY ONTOGENY IN DIFFERENT FORMS OF SILVER BIRCH Transactions of the Karelian Research Centre of the Russian Academy of Sciences betula pendula roth sucrose synthase apoplastic invertase vacuolar invertase cytosolic invertase |
| title | ACTIVITY OF SUCROSE DISSIMILATING ENZYMES IN EARLY ONTOGENY IN DIFFERENT FORMS OF SILVER BIRCH |
| title_full | ACTIVITY OF SUCROSE DISSIMILATING ENZYMES IN EARLY ONTOGENY IN DIFFERENT FORMS OF SILVER BIRCH |
| title_fullStr | ACTIVITY OF SUCROSE DISSIMILATING ENZYMES IN EARLY ONTOGENY IN DIFFERENT FORMS OF SILVER BIRCH |
| title_full_unstemmed | ACTIVITY OF SUCROSE DISSIMILATING ENZYMES IN EARLY ONTOGENY IN DIFFERENT FORMS OF SILVER BIRCH |
| title_short | ACTIVITY OF SUCROSE DISSIMILATING ENZYMES IN EARLY ONTOGENY IN DIFFERENT FORMS OF SILVER BIRCH |
| title_sort | activity of sucrose dissimilating enzymes in early ontogeny in different forms of silver birch |
| topic | betula pendula roth sucrose synthase apoplastic invertase vacuolar invertase cytosolic invertase |
| url | http://journals.krc.karelia.ru/index.php/biology/article/view/461 |
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