Chemico-Pharmacological Screening of the Methanol Extract of <i>Gynura nepalensis</i> D.C. Deciphered Promising Antioxidant and Hepatoprotective Potentials: Evidenced from <i>in vitro</i>, <i>in vivo</i>, and Computer-Aided Studies
<i>Gynura nepalensis</i> D.C. (family: Asteraceae) has abundant uses in the alternative medicinal practice, and this species is commonly used in the treatment of diabetes, rheumatism, cuts or wounds, asthma, kidney stones, cough, urinary tract bleeding, gall bladder stones, hepatitis, di...
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MDPI AG
2022-05-01
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| author | Nishan Chakrabarty Hea-Jong Chung Rashedul Alam Nazim Uddin Emon Safaet Alam Mohammed Fazlul Kabir Md. Minarul Islam Seong-Tshool Hong Tapas Sarkar Md. Moklesur Rahman Sarker Mohammad Manjur Rahman |
| author_facet | Nishan Chakrabarty Hea-Jong Chung Rashedul Alam Nazim Uddin Emon Safaet Alam Mohammed Fazlul Kabir Md. Minarul Islam Seong-Tshool Hong Tapas Sarkar Md. Moklesur Rahman Sarker Mohammad Manjur Rahman |
| author_sort | Nishan Chakrabarty |
| collection | DOAJ |
| description | <i>Gynura nepalensis</i> D.C. (family: Asteraceae) has abundant uses in the alternative medicinal practice, and this species is commonly used in the treatment of diabetes, rheumatism, cuts or wounds, asthma, kidney stones, cough, urinary tract bleeding, gall bladder stones, hepatitis, diarrhea, hemorrhoids, constipation, vomiting, fertility problems, blood poisoning, septicemia, skin allergy, indigestion, high cholesterol levels, and so on. This study aims to investigate the hepatoprotective and antioxidant potential of the methanol extract of the <i>Gynura nepalensis</i> D.C. (GNME) along with chemical profiling with phytochemical screening. Moreover, prospective phytocompounds have been screened virtually to present the binding affinity of the bioactive components to the hepatic and oxidative receptors. In the hepatoprotective study, alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total protein (TP), and lipid peroxidation (LP) and total bilirubin (TB) have been assessed, and in the antioxidant study, the DPPH free radical scavenging, total antioxidant flavonoid, and phenolic contents were determined. Moreover, the molecular binding affinity of the bioactive component of the plant has been analyzed using PyRx AutoDock Vina, Chimera, and Discovery Studio software. The plant extract showed dose-dependent hepatoprotective potential (<i>p</i> < 0.05, 0.01, 0.001) as well as strong antioxidant properties. Moreover, hepatoprotective and antioxidant molecular docking studies revealed a result varying from −2.90 kcal/mol to −10.1 kcal/mol. 4,5-dicaffeoylquinic acid and chlorogenic acid revealed the highest binding affinity among the selected molecules. However, the plant showed portent antioxidant and hepatoprotective properties in the in vitro, in vivo, and in silico models, and it is presumed that the hepatoprotective properties of the plant extract have occurred due to the presence of the vast bioactive chemical compounds as well as their antioxidant properties. Therefore, advanced studies are recommended to elucidate the pharmacological properties of the plant extracts. |
| first_indexed | 2024-03-10T01:04:47Z |
| format | Article |
| id | doaj.art-c2ea2517ed8d4c07ab9f08e494c42ce1 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T01:04:47Z |
| publishDate | 2022-05-01 |
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| series | Molecules |
| spelling | doaj.art-c2ea2517ed8d4c07ab9f08e494c42ce12023-11-23T14:29:19ZengMDPI AGMolecules1420-30492022-05-012711347410.3390/molecules27113474Chemico-Pharmacological Screening of the Methanol Extract of <i>Gynura nepalensis</i> D.C. Deciphered Promising Antioxidant and Hepatoprotective Potentials: Evidenced from <i>in vitro</i>, <i>in vivo</i>, and Computer-Aided StudiesNishan Chakrabarty0Hea-Jong Chung1Rashedul Alam2Nazim Uddin Emon3Safaet Alam4Mohammed Fazlul Kabir5Md. Minarul Islam6Seong-Tshool Hong7Tapas Sarkar8Md. Moklesur Rahman Sarker9Mohammad Manjur Rahman10Department of Pharmacy, Jagannath University, Dhaka 1100, BangladeshGwanju Center, Korea Basic Science Institute, Gwanju 61715, KoreaDepartment of Pharmacology, Medical School, Jeonbuk National University, Jeonju 54896, KoreaDepartment of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, Chattogram 4318, BangladeshPharmaceutical Sciences Research Division, Bangladesh Council of Scientific and Industrial Research Laboratories (BCSIR), Dr. Qudrat-I-Khuda Road, Dhanmondi, Dhaka 1205, BangladeshDepartment of Biology, Georgia State University, Atlanta, GA 30303, USADepartment of Biomedical Sciences, Institute for Medical Science, Jeonbuk National University Medical School, Jeonju 54907, KoreaDepartment of Biomedical Sciences, Institute for Medical Science, Jeonbuk National University Medical School, Jeonju 54907, KoreaDepartment of Pharmacy, Gono Bishwabidyalay, Dhaka 1344, BangladeshDepartment of Pharmacy, State University of Bangladesh, Dhaka 1205, BangladeshDepartment of Pharmacy, Gono Bishwabidyalay, Dhaka 1344, Bangladesh<i>Gynura nepalensis</i> D.C. (family: Asteraceae) has abundant uses in the alternative medicinal practice, and this species is commonly used in the treatment of diabetes, rheumatism, cuts or wounds, asthma, kidney stones, cough, urinary tract bleeding, gall bladder stones, hepatitis, diarrhea, hemorrhoids, constipation, vomiting, fertility problems, blood poisoning, septicemia, skin allergy, indigestion, high cholesterol levels, and so on. This study aims to investigate the hepatoprotective and antioxidant potential of the methanol extract of the <i>Gynura nepalensis</i> D.C. (GNME) along with chemical profiling with phytochemical screening. Moreover, prospective phytocompounds have been screened virtually to present the binding affinity of the bioactive components to the hepatic and oxidative receptors. In the hepatoprotective study, alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total protein (TP), and lipid peroxidation (LP) and total bilirubin (TB) have been assessed, and in the antioxidant study, the DPPH free radical scavenging, total antioxidant flavonoid, and phenolic contents were determined. Moreover, the molecular binding affinity of the bioactive component of the plant has been analyzed using PyRx AutoDock Vina, Chimera, and Discovery Studio software. The plant extract showed dose-dependent hepatoprotective potential (<i>p</i> < 0.05, 0.01, 0.001) as well as strong antioxidant properties. Moreover, hepatoprotective and antioxidant molecular docking studies revealed a result varying from −2.90 kcal/mol to −10.1 kcal/mol. 4,5-dicaffeoylquinic acid and chlorogenic acid revealed the highest binding affinity among the selected molecules. However, the plant showed portent antioxidant and hepatoprotective properties in the in vitro, in vivo, and in silico models, and it is presumed that the hepatoprotective properties of the plant extract have occurred due to the presence of the vast bioactive chemical compounds as well as their antioxidant properties. Therefore, advanced studies are recommended to elucidate the pharmacological properties of the plant extracts.https://www.mdpi.com/1420-3049/27/11/3474<i>Gynura nepalensis</i>hepatoprotectiveantioxidantbioactive compoundphytomedicinebinding affinity |
| spellingShingle | Nishan Chakrabarty Hea-Jong Chung Rashedul Alam Nazim Uddin Emon Safaet Alam Mohammed Fazlul Kabir Md. Minarul Islam Seong-Tshool Hong Tapas Sarkar Md. Moklesur Rahman Sarker Mohammad Manjur Rahman Chemico-Pharmacological Screening of the Methanol Extract of <i>Gynura nepalensis</i> D.C. Deciphered Promising Antioxidant and Hepatoprotective Potentials: Evidenced from <i>in vitro</i>, <i>in vivo</i>, and Computer-Aided Studies Molecules <i>Gynura nepalensis</i> hepatoprotective antioxidant bioactive compound phytomedicine binding affinity |
| title | Chemico-Pharmacological Screening of the Methanol Extract of <i>Gynura nepalensis</i> D.C. Deciphered Promising Antioxidant and Hepatoprotective Potentials: Evidenced from <i>in vitro</i>, <i>in vivo</i>, and Computer-Aided Studies |
| title_full | Chemico-Pharmacological Screening of the Methanol Extract of <i>Gynura nepalensis</i> D.C. Deciphered Promising Antioxidant and Hepatoprotective Potentials: Evidenced from <i>in vitro</i>, <i>in vivo</i>, and Computer-Aided Studies |
| title_fullStr | Chemico-Pharmacological Screening of the Methanol Extract of <i>Gynura nepalensis</i> D.C. Deciphered Promising Antioxidant and Hepatoprotective Potentials: Evidenced from <i>in vitro</i>, <i>in vivo</i>, and Computer-Aided Studies |
| title_full_unstemmed | Chemico-Pharmacological Screening of the Methanol Extract of <i>Gynura nepalensis</i> D.C. Deciphered Promising Antioxidant and Hepatoprotective Potentials: Evidenced from <i>in vitro</i>, <i>in vivo</i>, and Computer-Aided Studies |
| title_short | Chemico-Pharmacological Screening of the Methanol Extract of <i>Gynura nepalensis</i> D.C. Deciphered Promising Antioxidant and Hepatoprotective Potentials: Evidenced from <i>in vitro</i>, <i>in vivo</i>, and Computer-Aided Studies |
| title_sort | chemico pharmacological screening of the methanol extract of i gynura nepalensis i d c deciphered promising antioxidant and hepatoprotective potentials evidenced from i in vitro i i in vivo i and computer aided studies |
| topic | <i>Gynura nepalensis</i> hepatoprotective antioxidant bioactive compound phytomedicine binding affinity |
| url | https://www.mdpi.com/1420-3049/27/11/3474 |
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