High-sensitivity detection of optogenetically-induced neural activity with functional ultrasound imaging
Whole-brain imaging approaches and optogenetic manipulations are powerful tools to map brain-wide neural circuits in vivo. To date, functional magnetic resonance imaging (fMRI) provides the most comprehensive evaluation of such large-scale circuitry. However, functional ultrasound imaging (fUSI) has...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-11-01
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| Series: | NeuroImage |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1053811921007096 |
| _version_ | 1818669063734296576 |
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| author | Bradley Jay Edelman Giovanna D. Ielacqua Russell W. Chan Mazen Asaad Mankin Choy Jin Hyung Lee |
| author_facet | Bradley Jay Edelman Giovanna D. Ielacqua Russell W. Chan Mazen Asaad Mankin Choy Jin Hyung Lee |
| author_sort | Bradley Jay Edelman |
| collection | DOAJ |
| description | Whole-brain imaging approaches and optogenetic manipulations are powerful tools to map brain-wide neural circuits in vivo. To date, functional magnetic resonance imaging (fMRI) provides the most comprehensive evaluation of such large-scale circuitry. However, functional ultrasound imaging (fUSI) has recently emerged as a complementary imaging modality that can extend such measurements towards the context of diverse behavioral states and tasks. Nevertheless, in order to properly interpret the fUSI signal during these complicated scenarios, it must first be carefully validated against well-established technologies, such as fMRI, in highly controlled experimental settings. Here, to address this need, we compared subsequent fMRI and fUSI recordings in response to direct neuronal activation via optogenetics in the same animals under an identical anesthetic protocol. Specifically, we applied various intensities of light stimulation to the primary motor cortex (M1) of mice and compared the spatiotemporal dynamics of the elicited fMRI and fUSI signals. Overall, our general linear model analysis (t-scores) and time series analysis (z-scores) revealed that fUSI was more sensitive than fMRI for detecting optogenetically-induced neuronal activation. Local field potential recordings in the bilateral M1 and striatum also better co-localized with fUSI activation patterns than those of fMRI. Finally, the fUSI response contained distinct arterial and venous components that provide vascular readouts of neuronal activity with vessel-type specificity. |
| first_indexed | 2024-12-17T06:46:16Z |
| format | Article |
| id | doaj.art-c2eafdacdf954b7497ba533daf155b05 |
| institution | Directory Open Access Journal |
| issn | 1095-9572 |
| language | English |
| last_indexed | 2024-12-17T06:46:16Z |
| publishDate | 2021-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | NeuroImage |
| spelling | doaj.art-c2eafdacdf954b7497ba533daf155b052022-12-21T21:59:44ZengElsevierNeuroImage1095-95722021-11-01242118434High-sensitivity detection of optogenetically-induced neural activity with functional ultrasound imagingBradley Jay Edelman0Giovanna D. Ielacqua1Russell W. Chan2Mazen Asaad3Mankin Choy4Jin Hyung Lee5Department of Neurology and Neurological Sciences, Stanford University, Stanford, CA 94305, USADepartment of Neurology and Neurological Sciences, Stanford University, Stanford, CA 94305, USADepartment of Neurology and Neurological Sciences, Stanford University, Stanford, CA 94305, USADepartment of Neurology and Neurological Sciences, Stanford University, Stanford, CA 94305, USA; Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA, 94305, USADepartment of Neurology and Neurological Sciences, Stanford University, Stanford, CA 94305, USADepartment of Neurology and Neurological Sciences, Stanford University, Stanford, CA 94305, USA; Department of Bioengineering, Stanford University, Stanford, CA, 94305, USA; Department of Electrical Engineering, Stanford University, CA, 94305, USA; Department of Neurosurgery, Stanford University, Stanford, CA, 94305, USA; Corresponding author at: 318 Campus Drive, Clark Center, W300A, Stanford, CA 94305, USA.Whole-brain imaging approaches and optogenetic manipulations are powerful tools to map brain-wide neural circuits in vivo. To date, functional magnetic resonance imaging (fMRI) provides the most comprehensive evaluation of such large-scale circuitry. However, functional ultrasound imaging (fUSI) has recently emerged as a complementary imaging modality that can extend such measurements towards the context of diverse behavioral states and tasks. Nevertheless, in order to properly interpret the fUSI signal during these complicated scenarios, it must first be carefully validated against well-established technologies, such as fMRI, in highly controlled experimental settings. Here, to address this need, we compared subsequent fMRI and fUSI recordings in response to direct neuronal activation via optogenetics in the same animals under an identical anesthetic protocol. Specifically, we applied various intensities of light stimulation to the primary motor cortex (M1) of mice and compared the spatiotemporal dynamics of the elicited fMRI and fUSI signals. Overall, our general linear model analysis (t-scores) and time series analysis (z-scores) revealed that fUSI was more sensitive than fMRI for detecting optogenetically-induced neuronal activation. Local field potential recordings in the bilateral M1 and striatum also better co-localized with fUSI activation patterns than those of fMRI. Finally, the fUSI response contained distinct arterial and venous components that provide vascular readouts of neuronal activity with vessel-type specificity.http://www.sciencedirect.com/science/article/pii/S1053811921007096functional ultrasound imagingFunctional magnetic resonance imagingOptogeneticsNeural circuitsMotor cortex |
| spellingShingle | Bradley Jay Edelman Giovanna D. Ielacqua Russell W. Chan Mazen Asaad Mankin Choy Jin Hyung Lee High-sensitivity detection of optogenetically-induced neural activity with functional ultrasound imaging NeuroImage functional ultrasound imaging Functional magnetic resonance imaging Optogenetics Neural circuits Motor cortex |
| title | High-sensitivity detection of optogenetically-induced neural activity with functional ultrasound imaging |
| title_full | High-sensitivity detection of optogenetically-induced neural activity with functional ultrasound imaging |
| title_fullStr | High-sensitivity detection of optogenetically-induced neural activity with functional ultrasound imaging |
| title_full_unstemmed | High-sensitivity detection of optogenetically-induced neural activity with functional ultrasound imaging |
| title_short | High-sensitivity detection of optogenetically-induced neural activity with functional ultrasound imaging |
| title_sort | high sensitivity detection of optogenetically induced neural activity with functional ultrasound imaging |
| topic | functional ultrasound imaging Functional magnetic resonance imaging Optogenetics Neural circuits Motor cortex |
| url | http://www.sciencedirect.com/science/article/pii/S1053811921007096 |
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