Analysis of relative nuclear DNA content in carnation (Dianthus caryophyllus) accessions reveals ploidy levels by flow cytometry
Carnation (Dianthus caryophyllus L.) is one of the fifth most important ornamental species worldwide. Many desirable plant characteristics such as big size flower, adaptation to stress, and intra or interspecific hybridization capability are dependent on plant ploidy level. We optimized a quick flow...
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| Language: | English |
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Indian Council of Agricultural Research
2016-11-01
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| Series: | The Indian Journal of Agricultural Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAgS/article/view/62930 |
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| author | H ANSAR M V DHANANAJAYA AMREEN TAJ B FAKRUDIN A REKHA RAJIV KUMAR G K HALESH |
| author_facet | H ANSAR M V DHANANAJAYA AMREEN TAJ B FAKRUDIN A REKHA RAJIV KUMAR G K HALESH |
| author_sort | H ANSAR |
| collection | DOAJ |
| description | Carnation (Dianthus caryophyllus L.) is one of the fifth most important ornamental species worldwide. Many desirable plant characteristics such as big size flower, adaptation to stress, and intra or interspecific hybridization capability are dependent on plant ploidy level. We optimized a quick flow cytometry method for DNA content determination in carnation accession samples that allowed a systematic evaluation of ploidy levels. To verify the actual ploidy levels, we counted chromosome numbers in the root tips of representative cultivar for each ploidy level. The relative nuclear DNA content was distributed into four kinds of discontinuous groups: 1.32 to 1.95 pg (group 1), 2.03 to 2.72 pg (group 2), 2.98 to 4.65 pg (group 3) and 5.33 pg (group 4) which might correspond to the following ploidy levels; diploid, triploid, tetraploid and hexaploid. The results showed that out of 60 carnation accessions, 33 were diploid, 5 were triploid, 21 were tetraploid and 1 was hexaploid. |
| first_indexed | 2024-03-12T22:44:32Z |
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| id | doaj.art-c2eb520640c347cfb2f9af455c7e99d4 |
| institution | Directory Open Access Journal |
| issn | 0019-5022 2394-3319 |
| language | English |
| last_indexed | 2024-03-12T22:44:32Z |
| publishDate | 2016-11-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | The Indian Journal of Agricultural Sciences |
| spelling | doaj.art-c2eb520640c347cfb2f9af455c7e99d42023-07-21T08:26:44ZengIndian Council of Agricultural ResearchThe Indian Journal of Agricultural Sciences0019-50222394-33192016-11-01861110.56093/ijas.v86i11.62930Analysis of relative nuclear DNA content in carnation (Dianthus caryophyllus) accessions reveals ploidy levels by flow cytometryH ANSAR0M V DHANANAJAYA1AMREEN TAJ2B FAKRUDIN3A REKHA4RAJIV KUMAR5G K HALESH6ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru, Karnataka 560 089ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru, Karnataka 560 089ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru, Karnataka 560 089ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru, Karnataka 560 089ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru, Karnataka 560 089ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru, Karnataka 560 089ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru, Karnataka 560 089Carnation (Dianthus caryophyllus L.) is one of the fifth most important ornamental species worldwide. Many desirable plant characteristics such as big size flower, adaptation to stress, and intra or interspecific hybridization capability are dependent on plant ploidy level. We optimized a quick flow cytometry method for DNA content determination in carnation accession samples that allowed a systematic evaluation of ploidy levels. To verify the actual ploidy levels, we counted chromosome numbers in the root tips of representative cultivar for each ploidy level. The relative nuclear DNA content was distributed into four kinds of discontinuous groups: 1.32 to 1.95 pg (group 1), 2.03 to 2.72 pg (group 2), 2.98 to 4.65 pg (group 3) and 5.33 pg (group 4) which might correspond to the following ploidy levels; diploid, triploid, tetraploid and hexaploid. The results showed that out of 60 carnation accessions, 33 were diploid, 5 were triploid, 21 were tetraploid and 1 was hexaploid.https://epubs.icar.org.in/index.php/IJAgS/article/view/62930Carnation accessionsChromosome countingFlow cytometryPloidy level |
| spellingShingle | H ANSAR M V DHANANAJAYA AMREEN TAJ B FAKRUDIN A REKHA RAJIV KUMAR G K HALESH Analysis of relative nuclear DNA content in carnation (Dianthus caryophyllus) accessions reveals ploidy levels by flow cytometry The Indian Journal of Agricultural Sciences Carnation accessions Chromosome counting Flow cytometry Ploidy level |
| title | Analysis of relative nuclear DNA content in carnation (Dianthus caryophyllus) accessions reveals ploidy levels by flow cytometry |
| title_full | Analysis of relative nuclear DNA content in carnation (Dianthus caryophyllus) accessions reveals ploidy levels by flow cytometry |
| title_fullStr | Analysis of relative nuclear DNA content in carnation (Dianthus caryophyllus) accessions reveals ploidy levels by flow cytometry |
| title_full_unstemmed | Analysis of relative nuclear DNA content in carnation (Dianthus caryophyllus) accessions reveals ploidy levels by flow cytometry |
| title_short | Analysis of relative nuclear DNA content in carnation (Dianthus caryophyllus) accessions reveals ploidy levels by flow cytometry |
| title_sort | analysis of relative nuclear dna content in carnation dianthus caryophyllus accessions reveals ploidy levels by flow cytometry |
| topic | Carnation accessions Chromosome counting Flow cytometry Ploidy level |
| url | https://epubs.icar.org.in/index.php/IJAgS/article/view/62930 |
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