Universal Method for the Purification of Recombinant AAV Vectors of Differing Serotypes
The generation of clinical good manufacturing practices (GMP)-grade adeno-associated virus (AAV) vectors requires purification strategies that support the generation of vectors of high purity, and that exhibit a good safety and efficacy profile. To date, most reported purification schemas are seroty...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-06-01
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| Series: | Molecular Therapy: Methods & Clinical Development |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2329050117301298 |
| _version_ | 1818696051878526976 |
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| author | Shelley A. Nass Maryellen A. Mattingly Denise A. Woodcock Brenda L. Burnham Jeffrey A. Ardinger Shayla E. Osmond Amy M. Frederick Abraham Scaria Seng H. Cheng Catherine R. O’Riordan |
| author_facet | Shelley A. Nass Maryellen A. Mattingly Denise A. Woodcock Brenda L. Burnham Jeffrey A. Ardinger Shayla E. Osmond Amy M. Frederick Abraham Scaria Seng H. Cheng Catherine R. O’Riordan |
| author_sort | Shelley A. Nass |
| collection | DOAJ |
| description | The generation of clinical good manufacturing practices (GMP)-grade adeno-associated virus (AAV) vectors requires purification strategies that support the generation of vectors of high purity, and that exhibit a good safety and efficacy profile. To date, most reported purification schemas are serotype dependent, requiring method development for each AAV gene therapy product. Here, we describe a platform purification process that is compatible with the purification of multiple AAV serotypes. The method generates vector preparations of high purity that are enriched for capsids with full vector genomes, and that minimizes the fractional content of empty capsids. The two-column purification method, a combination of affinity and ion exchange chromatographies, is compatible with a range of AAV serotypes generated by either the transient triple transfection method or the more scalable producer cell line platform. In summary, the adaptable purification method described can be used for the production of a variety of high-quality AAV vectors suitable for preclinical testing in animal models of diseases. Keywords: AAV purification, epitope swapping, analytical ultracentrifugation |
| first_indexed | 2024-12-17T13:55:14Z |
| format | Article |
| id | doaj.art-c2eeffac7fb04805a98db2eeec81dbf4 |
| institution | Directory Open Access Journal |
| issn | 2329-0501 |
| language | English |
| last_indexed | 2024-12-17T13:55:14Z |
| publishDate | 2018-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Therapy: Methods & Clinical Development |
| spelling | doaj.art-c2eeffac7fb04805a98db2eeec81dbf42022-12-21T21:45:56ZengElsevierMolecular Therapy: Methods & Clinical Development2329-05012018-06-0193346Universal Method for the Purification of Recombinant AAV Vectors of Differing SerotypesShelley A. Nass0Maryellen A. Mattingly1Denise A. Woodcock2Brenda L. Burnham3Jeffrey A. Ardinger4Shayla E. Osmond5Amy M. Frederick6Abraham Scaria7Seng H. Cheng8Catherine R. O’Riordan9Gene Therapy, Sanofi, 49 New York Avenue, Framingham, MA 01701, USAGene Therapy, Sanofi, 49 New York Avenue, Framingham, MA 01701, USAGene Therapy, Sanofi, 49 New York Avenue, Framingham, MA 01701, USAGene Therapy, Sanofi, 49 New York Avenue, Framingham, MA 01701, USAGene Therapy, Sanofi, 49 New York Avenue, Framingham, MA 01701, USAGene Therapy, Sanofi, 49 New York Avenue, Framingham, MA 01701, USAGene Therapy, Sanofi, 49 New York Avenue, Framingham, MA 01701, USAGene Therapy, Sanofi, 49 New York Avenue, Framingham, MA 01701, USAGene Therapy, Sanofi, 49 New York Avenue, Framingham, MA 01701, USAGene Therapy, Sanofi, 49 New York Avenue, Framingham, MA 01701, USA; Corresponding author: Catherine R. O’Riordan, PhD, Gene Therapy, Sanofi, 49 New York Avenue, Framingham, MA 01701, USA.The generation of clinical good manufacturing practices (GMP)-grade adeno-associated virus (AAV) vectors requires purification strategies that support the generation of vectors of high purity, and that exhibit a good safety and efficacy profile. To date, most reported purification schemas are serotype dependent, requiring method development for each AAV gene therapy product. Here, we describe a platform purification process that is compatible with the purification of multiple AAV serotypes. The method generates vector preparations of high purity that are enriched for capsids with full vector genomes, and that minimizes the fractional content of empty capsids. The two-column purification method, a combination of affinity and ion exchange chromatographies, is compatible with a range of AAV serotypes generated by either the transient triple transfection method or the more scalable producer cell line platform. In summary, the adaptable purification method described can be used for the production of a variety of high-quality AAV vectors suitable for preclinical testing in animal models of diseases. Keywords: AAV purification, epitope swapping, analytical ultracentrifugationhttp://www.sciencedirect.com/science/article/pii/S2329050117301298 |
| spellingShingle | Shelley A. Nass Maryellen A. Mattingly Denise A. Woodcock Brenda L. Burnham Jeffrey A. Ardinger Shayla E. Osmond Amy M. Frederick Abraham Scaria Seng H. Cheng Catherine R. O’Riordan Universal Method for the Purification of Recombinant AAV Vectors of Differing Serotypes Molecular Therapy: Methods & Clinical Development |
| title | Universal Method for the Purification of Recombinant AAV Vectors of Differing Serotypes |
| title_full | Universal Method for the Purification of Recombinant AAV Vectors of Differing Serotypes |
| title_fullStr | Universal Method for the Purification of Recombinant AAV Vectors of Differing Serotypes |
| title_full_unstemmed | Universal Method for the Purification of Recombinant AAV Vectors of Differing Serotypes |
| title_short | Universal Method for the Purification of Recombinant AAV Vectors of Differing Serotypes |
| title_sort | universal method for the purification of recombinant aav vectors of differing serotypes |
| url | http://www.sciencedirect.com/science/article/pii/S2329050117301298 |
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