Maize cryptochromes 1a1 and 1a2 promote seedling photomorphogenesis and shade resistance in Zea mays and Arabidopsis
Maize growth and development are regulated by light quality, intensity and photoperiod. Cryptochromes are blue/ultraviolet-A light receptors involved in stem elongation, shade avoidance, and photoperiodic flowering. To investigate the function of cryptochrome 1 (CRY1) in maize, where it is encoded b...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2023-08-01
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| Series: | Crop Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214514123000429 |
| _version_ | 1827853269764931584 |
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| author | Xiaocong Fan Shizhan Chen Wenjing Wu Meifang Song Guanghua Sun Shuaitao Yao Weimin Zhan Lei Yan Hongdan Li Yanpei Zhang Lijian Wang Kang Zhang Liangliang Jiang Jianping Yang Qinghua Yang |
| author_facet | Xiaocong Fan Shizhan Chen Wenjing Wu Meifang Song Guanghua Sun Shuaitao Yao Weimin Zhan Lei Yan Hongdan Li Yanpei Zhang Lijian Wang Kang Zhang Liangliang Jiang Jianping Yang Qinghua Yang |
| author_sort | Xiaocong Fan |
| collection | DOAJ |
| description | Maize growth and development are regulated by light quality, intensity and photoperiod. Cryptochromes are blue/ultraviolet-A light receptors involved in stem elongation, shade avoidance, and photoperiodic flowering. To investigate the function of cryptochrome 1 (CRY1) in maize, where it is encoded by ZmCRY1, we obtained two ZmCRY1a genes (ZmCRY1a1 and ZmCRY1a2), both of which share the highest similarity with other gramineous plants, in particular rice CRY1a by phylogenetic analysis. In Arabidopsis, overexpression of ZmCRY1a genes promoted seedling de-etiolation under blue and white light, resulting in dwarfing of mature plants. In seedlings of the maize inbred line Zong 31 (ZmCRY1a-OE), overexpression of ZmCRY1a genes caused a reduction in the mesocotyl and first leaf sheath lengths due to down-regulation of genes influencing cell elongation. In mature transgenic maize plants, plant height, ear height, and internode length decreased in response to overexpression of ZmCRY1a genes. Expression of ZmCRY1a were insensitive to low blue light (LBL)-induced shade avoidance syndrome (SAS) in Arabidopsis and maize. This prompted us to investigate the regulatory role of the gibberellin and auxin metabolic pathways in the response of ZmCRY1a genes to LBL treatment. We confirmed a link between ZmCRY1a expression and hormonal influence on the growth and development of maize under LBL-induced SAS. These results reveal that ZmCRY1a has a relatively conservative function in regulating maize photomorphogenesis and may guide new strategies for breeding high density-tolerant maize cultivars. |
| first_indexed | 2024-03-12T11:02:51Z |
| format | Article |
| id | doaj.art-c2f052b9f175419892382d98d1eb1ee6 |
| institution | Directory Open Access Journal |
| issn | 2214-5141 |
| language | English |
| last_indexed | 2024-03-12T11:02:51Z |
| publishDate | 2023-08-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Crop Journal |
| spelling | doaj.art-c2f052b9f175419892382d98d1eb1ee62023-09-02T04:31:36ZengKeAi Communications Co., Ltd.Crop Journal2214-51412023-08-0111411921203Maize cryptochromes 1a1 and 1a2 promote seedling photomorphogenesis and shade resistance in Zea mays and ArabidopsisXiaocong Fan0Shizhan Chen1Wenjing Wu2Meifang Song3Guanghua Sun4Shuaitao Yao5Weimin Zhan6Lei Yan7Hongdan Li8Yanpei Zhang9 Lijian Wang10Kang Zhang11Liangliang Jiang12Jianping Yang13Qinghua Yang14College of Agronomy, State Key Laboratory of Wheat and Maize Crop Science, and Center for Crop Genome Engineering, Longzi Lake Campus, Henan Agricultural University, Zhengzhou 450046, Henan, ChinaCollege of Agronomy, State Key Laboratory of Wheat and Maize Crop Science, and Center for Crop Genome Engineering, Longzi Lake Campus, Henan Agricultural University, Zhengzhou 450046, Henan, ChinaCollege of Agronomy, State Key Laboratory of Wheat and Maize Crop Science, and Center for Crop Genome Engineering, Longzi Lake Campus, Henan Agricultural University, Zhengzhou 450046, Henan, ChinaInstitute of Radiation Technology, Beijing Academy of Science and Technology, Beijing 100875, ChinaCollege of Agronomy, State Key Laboratory of Wheat and Maize Crop Science, and Center for Crop Genome Engineering, Longzi Lake Campus, Henan Agricultural University, Zhengzhou 450046, Henan, ChinaCollege of Agronomy, State Key Laboratory of Wheat and Maize Crop Science, and Center for Crop Genome Engineering, Longzi Lake Campus, Henan Agricultural University, Zhengzhou 450046, Henan, ChinaCollege of Agronomy, State Key Laboratory of Wheat and Maize Crop Science, and Center for Crop Genome Engineering, Longzi Lake Campus, Henan Agricultural University, Zhengzhou 450046, Henan, ChinaInstitute of Crops, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, Jiangxi, ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaCollege of Agronomy, State Key Laboratory of Wheat and Maize Crop Science, and Center for Crop Genome Engineering, Longzi Lake Campus, Henan Agricultural University, Zhengzhou 450046, Henan, ChinaCollege of Agronomy, State Key Laboratory of Wheat and Maize Crop Science, and Center for Crop Genome Engineering, Longzi Lake Campus, Henan Agricultural University, Zhengzhou 450046, Henan, ChinaGenovo Biotechnology Co., Ltd., Tianjin 301700, ChinaCollege of Agronomy, State Key Laboratory of Wheat and Maize Crop Science, and Center for Crop Genome Engineering, Longzi Lake Campus, Henan Agricultural University, Zhengzhou 450046, Henan, China; Henan Institute of Science and Technology, Xinxiang 453003, Henan, China; Corresponding authors.College of Agronomy, State Key Laboratory of Wheat and Maize Crop Science, and Center for Crop Genome Engineering, Longzi Lake Campus, Henan Agricultural University, Zhengzhou 450046, Henan, China; Corresponding authors.College of Agronomy, State Key Laboratory of Wheat and Maize Crop Science, and Center for Crop Genome Engineering, Longzi Lake Campus, Henan Agricultural University, Zhengzhou 450046, Henan, China; Corresponding authors.Maize growth and development are regulated by light quality, intensity and photoperiod. Cryptochromes are blue/ultraviolet-A light receptors involved in stem elongation, shade avoidance, and photoperiodic flowering. To investigate the function of cryptochrome 1 (CRY1) in maize, where it is encoded by ZmCRY1, we obtained two ZmCRY1a genes (ZmCRY1a1 and ZmCRY1a2), both of which share the highest similarity with other gramineous plants, in particular rice CRY1a by phylogenetic analysis. In Arabidopsis, overexpression of ZmCRY1a genes promoted seedling de-etiolation under blue and white light, resulting in dwarfing of mature plants. In seedlings of the maize inbred line Zong 31 (ZmCRY1a-OE), overexpression of ZmCRY1a genes caused a reduction in the mesocotyl and first leaf sheath lengths due to down-regulation of genes influencing cell elongation. In mature transgenic maize plants, plant height, ear height, and internode length decreased in response to overexpression of ZmCRY1a genes. Expression of ZmCRY1a were insensitive to low blue light (LBL)-induced shade avoidance syndrome (SAS) in Arabidopsis and maize. This prompted us to investigate the regulatory role of the gibberellin and auxin metabolic pathways in the response of ZmCRY1a genes to LBL treatment. We confirmed a link between ZmCRY1a expression and hormonal influence on the growth and development of maize under LBL-induced SAS. These results reveal that ZmCRY1a has a relatively conservative function in regulating maize photomorphogenesis and may guide new strategies for breeding high density-tolerant maize cultivars.http://www.sciencedirect.com/science/article/pii/S2214514123000429Zea mays L.CryptochromePhotomorphogenesisShade avoidance syndromeHormone |
| spellingShingle | Xiaocong Fan Shizhan Chen Wenjing Wu Meifang Song Guanghua Sun Shuaitao Yao Weimin Zhan Lei Yan Hongdan Li Yanpei Zhang Lijian Wang Kang Zhang Liangliang Jiang Jianping Yang Qinghua Yang Maize cryptochromes 1a1 and 1a2 promote seedling photomorphogenesis and shade resistance in Zea mays and Arabidopsis Crop Journal Zea mays L. Cryptochrome Photomorphogenesis Shade avoidance syndrome Hormone |
| title | Maize cryptochromes 1a1 and 1a2 promote seedling photomorphogenesis and shade resistance in Zea mays and Arabidopsis |
| title_full | Maize cryptochromes 1a1 and 1a2 promote seedling photomorphogenesis and shade resistance in Zea mays and Arabidopsis |
| title_fullStr | Maize cryptochromes 1a1 and 1a2 promote seedling photomorphogenesis and shade resistance in Zea mays and Arabidopsis |
| title_full_unstemmed | Maize cryptochromes 1a1 and 1a2 promote seedling photomorphogenesis and shade resistance in Zea mays and Arabidopsis |
| title_short | Maize cryptochromes 1a1 and 1a2 promote seedling photomorphogenesis and shade resistance in Zea mays and Arabidopsis |
| title_sort | maize cryptochromes 1a1 and 1a2 promote seedling photomorphogenesis and shade resistance in zea mays and arabidopsis |
| topic | Zea mays L. Cryptochrome Photomorphogenesis Shade avoidance syndrome Hormone |
| url | http://www.sciencedirect.com/science/article/pii/S2214514123000429 |
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