Effects of Long-Term Triclosan Exposure on Microbiota in Zebrafish
Background: Triclosan (TCS) is a widely used antibacterial agent in personal care products and is ubiquitous in the environment. We aimed to examine whether TCS exposure affects microbiota in the gastrointestinal tract of zebrafish.Methods: After exposure to TCS 0 (Dimethyl Sulphoxide, DMSO control)...
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Frontiers Media S.A.
2021-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.604313/full |
| _version_ | 1819114276972920832 |
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| author | Ning Tang Pianpian Fan Xiaogang Yu Rui Ma Yexuan Tao Weiye Wang Fengxiu Ouyang |
| author_facet | Ning Tang Pianpian Fan Xiaogang Yu Rui Ma Yexuan Tao Weiye Wang Fengxiu Ouyang |
| author_sort | Ning Tang |
| collection | DOAJ |
| description | Background: Triclosan (TCS) is a widely used antibacterial agent in personal care products and is ubiquitous in the environment. We aimed to examine whether TCS exposure affects microbiota in the gastrointestinal tract of zebrafish.Methods: After exposure to TCS 0 (Dimethyl Sulphoxide, DMSO control), 0.03, 0.3, 3, 30, 100, and 300ng/ml, respectively, from day 0 to 120days post fertilization (dpf), or for 7days in adult 4-month zebrafish, the long- and short-term impact of TCS exposure on the microbiome in the gastrointestinal tract was evaluated by analyzing 16S rRNA gene V3-V4 region sequencing.Results: The top two most dominant microbiota phyla were Proteobacteria and Fusobacteria phylum in all zebrafish groups. In TCS exposure 0–120 dpf, compared with DMSO control, the mean number of microbial operational taxonomic units (OTUs) was 54.46 lower (p<0.0001), Chao indice 41.40 lower (p=0.0004), and Ace indice 34.10 lower (p=0.0044) in TCS 300ng/ml group, but no change was observed in most of the other TCS concentrations. PCoA diagram showed that the microbial community in the long-term TCS 300ng/ml exposure group clustered differently from those in the DMSO control and other TCS exposure groups. A shorter body length of the zebrafish was observed in the long-term TCS exposure at 0.03, 100, and 300ng/ml. For 7-day short-term exposure in adult zebrafish, no difference was observed in alpha or beta diversity of microbiota nor the relative abundance of Proteobacteria or Fusobacteria phylum among DMSO control and any TCS levels, but a minor difference in microbial composition was observed for TCS exposure.Conclusions: Long-term exposure to high TCS concentration in a window from early embryonic life to early adulthood may reduce diversity and alter the composition of microbiota in the gastrointestinal tract. The effect of short-term TCS exposure was not observed on the diversity of microbiota but there was a minor change of microbial composition in adult zebrafish with TCS exposure. |
| first_indexed | 2024-12-22T04:42:44Z |
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| id | doaj.art-c2f1dc6615724f8d96e6b69a03cb6b8a |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-22T04:42:44Z |
| publishDate | 2021-10-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-c2f1dc6615724f8d96e6b69a03cb6b8a2022-12-21T18:38:42ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-10-011210.3389/fmicb.2021.604313604313Effects of Long-Term Triclosan Exposure on Microbiota in ZebrafishNing Tang0Pianpian Fan1Xiaogang Yu2Rui Ma3Yexuan Tao4Weiye Wang5Fengxiu Ouyang6Ministry of Education and Shanghai Key Laboratory of Children’s Environmental Health, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaMinistry of Education and Shanghai Key Laboratory of Children’s Environmental Health, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaMinistry of Education and Shanghai Key Laboratory of Children’s Environmental Health, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaMinistry of Education and Shanghai Key Laboratory of Children’s Environmental Health, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Clinical Nutrition, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaMinistry of Education and Shanghai Key Laboratory of Children’s Environmental Health, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaMinistry of Education and Shanghai Key Laboratory of Children’s Environmental Health, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaBackground: Triclosan (TCS) is a widely used antibacterial agent in personal care products and is ubiquitous in the environment. We aimed to examine whether TCS exposure affects microbiota in the gastrointestinal tract of zebrafish.Methods: After exposure to TCS 0 (Dimethyl Sulphoxide, DMSO control), 0.03, 0.3, 3, 30, 100, and 300ng/ml, respectively, from day 0 to 120days post fertilization (dpf), or for 7days in adult 4-month zebrafish, the long- and short-term impact of TCS exposure on the microbiome in the gastrointestinal tract was evaluated by analyzing 16S rRNA gene V3-V4 region sequencing.Results: The top two most dominant microbiota phyla were Proteobacteria and Fusobacteria phylum in all zebrafish groups. In TCS exposure 0–120 dpf, compared with DMSO control, the mean number of microbial operational taxonomic units (OTUs) was 54.46 lower (p<0.0001), Chao indice 41.40 lower (p=0.0004), and Ace indice 34.10 lower (p=0.0044) in TCS 300ng/ml group, but no change was observed in most of the other TCS concentrations. PCoA diagram showed that the microbial community in the long-term TCS 300ng/ml exposure group clustered differently from those in the DMSO control and other TCS exposure groups. A shorter body length of the zebrafish was observed in the long-term TCS exposure at 0.03, 100, and 300ng/ml. For 7-day short-term exposure in adult zebrafish, no difference was observed in alpha or beta diversity of microbiota nor the relative abundance of Proteobacteria or Fusobacteria phylum among DMSO control and any TCS levels, but a minor difference in microbial composition was observed for TCS exposure.Conclusions: Long-term exposure to high TCS concentration in a window from early embryonic life to early adulthood may reduce diversity and alter the composition of microbiota in the gastrointestinal tract. The effect of short-term TCS exposure was not observed on the diversity of microbiota but there was a minor change of microbial composition in adult zebrafish with TCS exposure.https://www.frontiersin.org/articles/10.3389/fmicb.2021.604313/fullTriclosan (TCS)long-term exposuremicrobiota in the gastrointestinal tract16S rRNA geneearly lifezebrafish |
| spellingShingle | Ning Tang Pianpian Fan Xiaogang Yu Rui Ma Yexuan Tao Weiye Wang Fengxiu Ouyang Effects of Long-Term Triclosan Exposure on Microbiota in Zebrafish Frontiers in Microbiology Triclosan (TCS) long-term exposure microbiota in the gastrointestinal tract 16S rRNA gene early life zebrafish |
| title | Effects of Long-Term Triclosan Exposure on Microbiota in Zebrafish |
| title_full | Effects of Long-Term Triclosan Exposure on Microbiota in Zebrafish |
| title_fullStr | Effects of Long-Term Triclosan Exposure on Microbiota in Zebrafish |
| title_full_unstemmed | Effects of Long-Term Triclosan Exposure on Microbiota in Zebrafish |
| title_short | Effects of Long-Term Triclosan Exposure on Microbiota in Zebrafish |
| title_sort | effects of long term triclosan exposure on microbiota in zebrafish |
| topic | Triclosan (TCS) long-term exposure microbiota in the gastrointestinal tract 16S rRNA gene early life zebrafish |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.604313/full |
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