Mutation of YL Results in a Yellow Leaf with Chloroplast RNA Editing Defect in Soybean
RNA editing plays a key role in organelle gene expression. Little is known about how RNA editing factors influence soybean plant development. Here, we report the isolation and characterization of a soybean <i>yl</i> (<i>yellow leaf</i>) mutant. The <i>yl</i> plant...
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MDPI AG
2020-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/12/4275 |
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| author | Xiaowei Zhu Yi Pan Zhi Liu Yucheng Liu Deyi Zhong Zongbiao Duan Zhixi Tian Baoge Zhu Guoan Zhou |
| author_facet | Xiaowei Zhu Yi Pan Zhi Liu Yucheng Liu Deyi Zhong Zongbiao Duan Zhixi Tian Baoge Zhu Guoan Zhou |
| author_sort | Xiaowei Zhu |
| collection | DOAJ |
| description | RNA editing plays a key role in organelle gene expression. Little is known about how RNA editing factors influence soybean plant development. Here, we report the isolation and characterization of a soybean <i>yl</i> (<i>yellow leaf</i>) mutant. The <i>yl</i> plants showed decreased chlorophyll accumulation, lower PS II activity, an impaired net photosynthesis rate, and an altered chloroplast ultrastructure. Fine mapping of <i>YL</i> uncovered a point mutation in <i>Glyma.20G187000</i>, which encodes a chloroplast-localized protein homologous to <i>Arabidopsis thaliana</i> (<i>Arabidopsis</i>) ORRM1. <i>YL</i> is mainly expressed in trifoliate leaves, and its deficiency affects the editing of multiple chloroplast RNA sites, leading to inferior photosynthesis in soybean. Taken together, these results demonstrate the importance of the soybean YL protein in chloroplast RNA editing and photosynthesis. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T19:09:32Z |
| publishDate | 2020-06-01 |
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| spelling | doaj.art-c2f4c92b53274d76a87fbc8c32c1feef2023-11-20T03:57:44ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-06-012112427510.3390/ijms21124275Mutation of YL Results in a Yellow Leaf with Chloroplast RNA Editing Defect in SoybeanXiaowei Zhu0Yi Pan1Zhi Liu2Yucheng Liu3Deyi Zhong4Zongbiao Duan5Zhixi Tian6Baoge Zhu7Guoan Zhou8State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, ChinaRNA editing plays a key role in organelle gene expression. Little is known about how RNA editing factors influence soybean plant development. Here, we report the isolation and characterization of a soybean <i>yl</i> (<i>yellow leaf</i>) mutant. The <i>yl</i> plants showed decreased chlorophyll accumulation, lower PS II activity, an impaired net photosynthesis rate, and an altered chloroplast ultrastructure. Fine mapping of <i>YL</i> uncovered a point mutation in <i>Glyma.20G187000</i>, which encodes a chloroplast-localized protein homologous to <i>Arabidopsis thaliana</i> (<i>Arabidopsis</i>) ORRM1. <i>YL</i> is mainly expressed in trifoliate leaves, and its deficiency affects the editing of multiple chloroplast RNA sites, leading to inferior photosynthesis in soybean. Taken together, these results demonstrate the importance of the soybean YL protein in chloroplast RNA editing and photosynthesis.https://www.mdpi.com/1422-0067/21/12/4275soybean<i>yellow leaf</i> mutantphotosynthesischloroplast RNA editing |
| spellingShingle | Xiaowei Zhu Yi Pan Zhi Liu Yucheng Liu Deyi Zhong Zongbiao Duan Zhixi Tian Baoge Zhu Guoan Zhou Mutation of YL Results in a Yellow Leaf with Chloroplast RNA Editing Defect in Soybean International Journal of Molecular Sciences soybean <i>yellow leaf</i> mutant photosynthesis chloroplast RNA editing |
| title | Mutation of YL Results in a Yellow Leaf with Chloroplast RNA Editing Defect in Soybean |
| title_full | Mutation of YL Results in a Yellow Leaf with Chloroplast RNA Editing Defect in Soybean |
| title_fullStr | Mutation of YL Results in a Yellow Leaf with Chloroplast RNA Editing Defect in Soybean |
| title_full_unstemmed | Mutation of YL Results in a Yellow Leaf with Chloroplast RNA Editing Defect in Soybean |
| title_short | Mutation of YL Results in a Yellow Leaf with Chloroplast RNA Editing Defect in Soybean |
| title_sort | mutation of yl results in a yellow leaf with chloroplast rna editing defect in soybean |
| topic | soybean <i>yellow leaf</i> mutant photosynthesis chloroplast RNA editing |
| url | https://www.mdpi.com/1422-0067/21/12/4275 |
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