Biomass Deacetylation at Moderate Solid Loading Improves Sugar Recovery and Succinic Acid Production
Biomass deacetylation with alkali prior to dilute acid pretreatment can be a promising approach to reduce the toxicity of the resulting hydrolysates and improve microbial fermentation. In this study, the effect of mild alkaline treatment of oil palm trunk (OPT) biomass on succinic acid production wa...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/3/235 |
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| author | Nurul Adela Bukhari Abdullah Amru Indera Luthfi Nuraishah Abd Rahim Abu Bakar Nasrin Mohamad Azri Sukiran Soh Kheang Loh |
| author_facet | Nurul Adela Bukhari Abdullah Amru Indera Luthfi Nuraishah Abd Rahim Abu Bakar Nasrin Mohamad Azri Sukiran Soh Kheang Loh |
| author_sort | Nurul Adela Bukhari |
| collection | DOAJ |
| description | Biomass deacetylation with alkali prior to dilute acid pretreatment can be a promising approach to reduce the toxicity of the resulting hydrolysates and improve microbial fermentation. In this study, the effect of mild alkaline treatment of oil palm trunk (OPT) biomass on succinic acid production was evaluated. Deacetylation was carried out under different conditions: NaOH loadings (1–5%, <i>w</i>/<i>v</i>) and reaction times (15–90 min) at 100 °C. Deacetylation using 1% (<i>w</i>/<i>v</i>) NaOH within 15 min was sufficient to achieve a high acetic acid removal of 5.8 g/L with minimal sugar loss. Deacetylation under this condition resulted in a total sugar concentration of 55.8 g/L (18.0 g/L xylose and 37.8 g/L glucose), which was 37% higher than that of non-deacetylated OPT. Subsequently, succinic acid production using <i>Actinobacillus succinogenes</i> was also improved by 42% and 13% in terms of productivity and yield, respectively, at 10% (<i>w</i>/<i>v</i>) solid loading. This further demonstrated that mild alkaline treatment prior to dilute acid pretreatment is a promising strategy to improve succinic acid production. This study provides a facile approach for reducing the most influential inhibitory effect of acetic acid, and it can be applied to the exploitation of lignocellulosic biomass resources for succinic acid, biofuels, and/or other biochemical co-production in the future. |
| first_indexed | 2024-03-11T06:34:17Z |
| format | Article |
| id | doaj.art-c2f6cb1695b747329b73e5395cd5b27a |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T06:34:17Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-c2f6cb1695b747329b73e5395cd5b27a2023-11-17T11:01:52ZengMDPI AGFermentation2311-56372023-02-019323510.3390/fermentation9030235Biomass Deacetylation at Moderate Solid Loading Improves Sugar Recovery and Succinic Acid ProductionNurul Adela Bukhari0Abdullah Amru Indera Luthfi1Nuraishah Abd Rahim2Abu Bakar Nasrin3Mohamad Azri Sukiran4Soh Kheang Loh5Energy and Environment Unit, Engineering & Processing Research Division, Malaysian Palm Oil Board (MPOB), 6, Persiaran Institusi, Bandar Baru Bangi, Kajang 43000, Selangor, MalaysiaDepartment of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, MalaysiaDepartment of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, MalaysiaEnergy and Environment Unit, Engineering & Processing Research Division, Malaysian Palm Oil Board (MPOB), 6, Persiaran Institusi, Bandar Baru Bangi, Kajang 43000, Selangor, MalaysiaEnergy and Environment Unit, Engineering & Processing Research Division, Malaysian Palm Oil Board (MPOB), 6, Persiaran Institusi, Bandar Baru Bangi, Kajang 43000, Selangor, MalaysiaEnergy and Environment Unit, Engineering & Processing Research Division, Malaysian Palm Oil Board (MPOB), 6, Persiaran Institusi, Bandar Baru Bangi, Kajang 43000, Selangor, MalaysiaBiomass deacetylation with alkali prior to dilute acid pretreatment can be a promising approach to reduce the toxicity of the resulting hydrolysates and improve microbial fermentation. In this study, the effect of mild alkaline treatment of oil palm trunk (OPT) biomass on succinic acid production was evaluated. Deacetylation was carried out under different conditions: NaOH loadings (1–5%, <i>w</i>/<i>v</i>) and reaction times (15–90 min) at 100 °C. Deacetylation using 1% (<i>w</i>/<i>v</i>) NaOH within 15 min was sufficient to achieve a high acetic acid removal of 5.8 g/L with minimal sugar loss. Deacetylation under this condition resulted in a total sugar concentration of 55.8 g/L (18.0 g/L xylose and 37.8 g/L glucose), which was 37% higher than that of non-deacetylated OPT. Subsequently, succinic acid production using <i>Actinobacillus succinogenes</i> was also improved by 42% and 13% in terms of productivity and yield, respectively, at 10% (<i>w</i>/<i>v</i>) solid loading. This further demonstrated that mild alkaline treatment prior to dilute acid pretreatment is a promising strategy to improve succinic acid production. This study provides a facile approach for reducing the most influential inhibitory effect of acetic acid, and it can be applied to the exploitation of lignocellulosic biomass resources for succinic acid, biofuels, and/or other biochemical co-production in the future.https://www.mdpi.com/2311-5637/9/3/235lignocellulosic biomassoil palm trunkacetic acidfermentation<i>Actinobacillus succinogenes</i> |
| spellingShingle | Nurul Adela Bukhari Abdullah Amru Indera Luthfi Nuraishah Abd Rahim Abu Bakar Nasrin Mohamad Azri Sukiran Soh Kheang Loh Biomass Deacetylation at Moderate Solid Loading Improves Sugar Recovery and Succinic Acid Production Fermentation lignocellulosic biomass oil palm trunk acetic acid fermentation <i>Actinobacillus succinogenes</i> |
| title | Biomass Deacetylation at Moderate Solid Loading Improves Sugar Recovery and Succinic Acid Production |
| title_full | Biomass Deacetylation at Moderate Solid Loading Improves Sugar Recovery and Succinic Acid Production |
| title_fullStr | Biomass Deacetylation at Moderate Solid Loading Improves Sugar Recovery and Succinic Acid Production |
| title_full_unstemmed | Biomass Deacetylation at Moderate Solid Loading Improves Sugar Recovery and Succinic Acid Production |
| title_short | Biomass Deacetylation at Moderate Solid Loading Improves Sugar Recovery and Succinic Acid Production |
| title_sort | biomass deacetylation at moderate solid loading improves sugar recovery and succinic acid production |
| topic | lignocellulosic biomass oil palm trunk acetic acid fermentation <i>Actinobacillus succinogenes</i> |
| url | https://www.mdpi.com/2311-5637/9/3/235 |
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