Harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralization
Abstract Acidic and chemical inhibitor stresses undermine efficient lactic acid bioproduction from lignocellulosic feedstock. Requisite coping treatments, such as detoxification and neutralizing agent supplementation, can be eliminated if a strong microbial host is employed in the process. Here, we...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-17737-4 |
| _version_ | 1811215522798239744 |
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| author | Radityo Pangestu Prihardi Kahar Lutfi Nia Kholida Urip Perwitasari Ahmad Thontowi Fahrurrozi Puspita Lisdiyanti Yopi Chiaki Ogino Bambang Prasetya Akihiko Kondo |
| author_facet | Radityo Pangestu Prihardi Kahar Lutfi Nia Kholida Urip Perwitasari Ahmad Thontowi Fahrurrozi Puspita Lisdiyanti Yopi Chiaki Ogino Bambang Prasetya Akihiko Kondo |
| author_sort | Radityo Pangestu |
| collection | DOAJ |
| description | Abstract Acidic and chemical inhibitor stresses undermine efficient lactic acid bioproduction from lignocellulosic feedstock. Requisite coping treatments, such as detoxification and neutralizing agent supplementation, can be eliminated if a strong microbial host is employed in the process. Here, we exploited an originally robust yeast, Saccharomyces cerevisiae BTCC3, as a production platform for lactic acid. This wild-type strain exhibited a rapid cell growth in the presence of various chemical inhibitors compared to laboratory and industrial strains, namely BY4741 and Ethanol-red. Pathway engineering was performed on the strain by introducing an exogenous LDH gene after disrupting the PDC1 and PDC5 genes. Facilitated by this engineered strain, high cell density cultivation could generate lactic acid with productivity at 4.80 and 3.68 g L−1 h−1 under semi-neutralized and non-neutralized conditions, respectively. Those values were relatively higher compared to other studies. Cultivation using real lignocellulosic hydrolysate was conducted to assess the performance of this engineered strain. Non-neutralized fermentation using non-detoxified hydrolysate from sugarcane bagasse as a medium could produce lactic acid at 1.69 g L−1 h−1, which was competitive to the results from other reports that still included detoxification and neutralization steps in their experiments. This strategy could make the overall lactic acid bioproduction process simpler, greener, and more cost-efficient. |
| first_indexed | 2024-04-12T06:24:00Z |
| format | Article |
| id | doaj.art-c2f70fefb90e4ce0969188e9a66feff7 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T06:24:00Z |
| publishDate | 2022-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-c2f70fefb90e4ce0969188e9a66feff72022-12-22T03:44:13ZengNature PortfolioScientific Reports2045-23222022-08-0112111310.1038/s41598-022-17737-4Harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralizationRadityo Pangestu0Prihardi Kahar1Lutfi Nia Kholida2Urip Perwitasari3Ahmad Thontowi4Fahrurrozi5Puspita Lisdiyanti6Yopi7Chiaki Ogino8Bambang Prasetya9Akihiko Kondo10Graduate School of Engineering, Kobe UniversityGraduate School of Engineering, Kobe UniversityNational Research and Innovation Agency (BRIN)National Research and Innovation Agency (BRIN)National Research and Innovation Agency (BRIN)National Research and Innovation Agency (BRIN)National Research and Innovation Agency (BRIN)National Research and Innovation Agency (BRIN)Graduate School of Engineering, Kobe UniversityNational Research and Innovation Agency (BRIN)Graduate School of Engineering, Kobe UniversityAbstract Acidic and chemical inhibitor stresses undermine efficient lactic acid bioproduction from lignocellulosic feedstock. Requisite coping treatments, such as detoxification and neutralizing agent supplementation, can be eliminated if a strong microbial host is employed in the process. Here, we exploited an originally robust yeast, Saccharomyces cerevisiae BTCC3, as a production platform for lactic acid. This wild-type strain exhibited a rapid cell growth in the presence of various chemical inhibitors compared to laboratory and industrial strains, namely BY4741 and Ethanol-red. Pathway engineering was performed on the strain by introducing an exogenous LDH gene after disrupting the PDC1 and PDC5 genes. Facilitated by this engineered strain, high cell density cultivation could generate lactic acid with productivity at 4.80 and 3.68 g L−1 h−1 under semi-neutralized and non-neutralized conditions, respectively. Those values were relatively higher compared to other studies. Cultivation using real lignocellulosic hydrolysate was conducted to assess the performance of this engineered strain. Non-neutralized fermentation using non-detoxified hydrolysate from sugarcane bagasse as a medium could produce lactic acid at 1.69 g L−1 h−1, which was competitive to the results from other reports that still included detoxification and neutralization steps in their experiments. This strategy could make the overall lactic acid bioproduction process simpler, greener, and more cost-efficient.https://doi.org/10.1038/s41598-022-17737-4 |
| spellingShingle | Radityo Pangestu Prihardi Kahar Lutfi Nia Kholida Urip Perwitasari Ahmad Thontowi Fahrurrozi Puspita Lisdiyanti Yopi Chiaki Ogino Bambang Prasetya Akihiko Kondo Harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralization Scientific Reports |
| title | Harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralization |
| title_full | Harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralization |
| title_fullStr | Harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralization |
| title_full_unstemmed | Harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralization |
| title_short | Harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralization |
| title_sort | harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralization |
| url | https://doi.org/10.1038/s41598-022-17737-4 |
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