Simultaneous determination of atorvastatin and ezetimibe from combined pharmaceutical products by micellar electrokinetic capillary chromatography
Abstract A rapid and sensitive micellar electrokinetic capillary chromatography method with UV photodiode-array detection was developed for the simultaneous determination of atorvastatin and ezetimibe in fixed dose drug combination. Experimental conditions such as buffer concentration and pH, surfac...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade de São Paulo
2017-04-01
|
| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100626&lng=en&tlng=en |
| _version_ | 1811330298452901888 |
|---|---|
| author | Blanka Székely-Szentmiklósi Gabriel Hancu István Székely-Szentmiklósi Béla Kovács Hajnal Kelemen |
| author_facet | Blanka Székely-Szentmiklósi Gabriel Hancu István Székely-Szentmiklósi Béla Kovács Hajnal Kelemen |
| author_sort | Blanka Székely-Szentmiklósi |
| collection | DOAJ |
| description | Abstract A rapid and sensitive micellar electrokinetic capillary chromatography method with UV photodiode-array detection was developed for the simultaneous determination of atorvastatin and ezetimibe in fixed dose drug combination. Experimental conditions such as buffer concentration and pH, surfactant concentration, system temperature, applied voltage, injection parameters were optimized in order to improve the efficiency of the separation. The best results were obtained when using fused silica capillary (48 cm length X 50 µm ID) and 25 mM borate buffer electrolyte at pH 9.3 containing 25 mM SDS, + 30 kV applied voltage, 20 ºC system temperature. The separation was achieved in approximately 2 minutes, with a resolution of 7.02, the order of migration being atorvastatin followed by ezetimibe. The analytical performance of the method was verified with regard to linearity, precision, robustness and the limit of detection and quantification were calculated. |
| first_indexed | 2024-04-13T15:59:58Z |
| format | Article |
| id | doaj.art-c2f75b1b10d940a4a3cdea94d12d19fa |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-04-13T15:59:58Z |
| publishDate | 2017-04-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-c2f75b1b10d940a4a3cdea94d12d19fa2022-12-22T02:40:35ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902017-04-0153110.1590/s2175-97902017000116122S1984-82502017000100626Simultaneous determination of atorvastatin and ezetimibe from combined pharmaceutical products by micellar electrokinetic capillary chromatographyBlanka Székely-SzentmiklósiGabriel HancuIstván Székely-SzentmiklósiBéla KovácsHajnal KelemenAbstract A rapid and sensitive micellar electrokinetic capillary chromatography method with UV photodiode-array detection was developed for the simultaneous determination of atorvastatin and ezetimibe in fixed dose drug combination. Experimental conditions such as buffer concentration and pH, surfactant concentration, system temperature, applied voltage, injection parameters were optimized in order to improve the efficiency of the separation. The best results were obtained when using fused silica capillary (48 cm length X 50 µm ID) and 25 mM borate buffer electrolyte at pH 9.3 containing 25 mM SDS, + 30 kV applied voltage, 20 ºC system temperature. The separation was achieved in approximately 2 minutes, with a resolution of 7.02, the order of migration being atorvastatin followed by ezetimibe. The analytical performance of the method was verified with regard to linearity, precision, robustness and the limit of detection and quantification were calculated.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100626&lng=en&tlng=enMicellar Electrokinetic Capillary ChromatographyFixed Dose Drug CombinationAtorvastatinEzetimibe. |
| spellingShingle | Blanka Székely-Szentmiklósi Gabriel Hancu István Székely-Szentmiklósi Béla Kovács Hajnal Kelemen Simultaneous determination of atorvastatin and ezetimibe from combined pharmaceutical products by micellar electrokinetic capillary chromatography Brazilian Journal of Pharmaceutical Sciences Micellar Electrokinetic Capillary Chromatography Fixed Dose Drug Combination Atorvastatin Ezetimibe. |
| title | Simultaneous determination of atorvastatin and ezetimibe from combined pharmaceutical products by micellar electrokinetic capillary chromatography |
| title_full | Simultaneous determination of atorvastatin and ezetimibe from combined pharmaceutical products by micellar electrokinetic capillary chromatography |
| title_fullStr | Simultaneous determination of atorvastatin and ezetimibe from combined pharmaceutical products by micellar electrokinetic capillary chromatography |
| title_full_unstemmed | Simultaneous determination of atorvastatin and ezetimibe from combined pharmaceutical products by micellar electrokinetic capillary chromatography |
| title_short | Simultaneous determination of atorvastatin and ezetimibe from combined pharmaceutical products by micellar electrokinetic capillary chromatography |
| title_sort | simultaneous determination of atorvastatin and ezetimibe from combined pharmaceutical products by micellar electrokinetic capillary chromatography |
| topic | Micellar Electrokinetic Capillary Chromatography Fixed Dose Drug Combination Atorvastatin Ezetimibe. |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100626&lng=en&tlng=en |
| work_keys_str_mv | AT blankaszekelyszentmiklosi simultaneousdeterminationofatorvastatinandezetimibefromcombinedpharmaceuticalproductsbymicellarelectrokineticcapillarychromatography AT gabrielhancu simultaneousdeterminationofatorvastatinandezetimibefromcombinedpharmaceuticalproductsbymicellarelectrokineticcapillarychromatography AT istvanszekelyszentmiklosi simultaneousdeterminationofatorvastatinandezetimibefromcombinedpharmaceuticalproductsbymicellarelectrokineticcapillarychromatography AT belakovacs simultaneousdeterminationofatorvastatinandezetimibefromcombinedpharmaceuticalproductsbymicellarelectrokineticcapillarychromatography AT hajnalkelemen simultaneousdeterminationofatorvastatinandezetimibefromcombinedpharmaceuticalproductsbymicellarelectrokineticcapillarychromatography |