Assessment of Wild Rocket (<i>Diplotaxis tenuifolia</i> (L.) DC.) Germplasm Accessions by NGS Identified SSR and SNP Markers
Rocket is the common designation for two baby-leaf salad crops of the Brassicaceae family: <i>Eruca sativa</i> (L.) Cav., usually referred to as annual garden rocket, and <i>Diplotaxis tenuifolia</i> (L.) DC. commonly named to as perennial wild rocket. <i>E. sativa</...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/24/3482 |
| _version_ | 1797455677449306112 |
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| author | João M. Reis Ricardo J. Pereira Paula S. Coelho José M. Leitão |
| author_facet | João M. Reis Ricardo J. Pereira Paula S. Coelho José M. Leitão |
| author_sort | João M. Reis |
| collection | DOAJ |
| description | Rocket is the common designation for two baby-leaf salad crops of the Brassicaceae family: <i>Eruca sativa</i> (L.) Cav., usually referred to as annual garden rocket, and <i>Diplotaxis tenuifolia</i> (L.) DC. commonly named to as perennial wild rocket. <i>E. sativa</i> is used for human consumption since antiquity. However, the growing consumer preference for <i>D. tenuifolia</i> is being accompanied by the fast increase in its production area and commercialization of new cultivars. Nevertheless, the worldwide number of wild rocket accessions maintained in germplasm collections is very reduced, the solution for which situation the project “REMIRucula” intends to contribute, establishing a germplasm collection at the INIAV, Oeiras, Portugal. Herein, we report on the establishment via next generation sequencing (NGS) of the first genome assembly of <i>D. tenuifolia</i> and the identification of specific single sequence repeat (SSR) and single nucleotide polymorphisms (SNP) loci for the establishment of specific DNA-markers for this species. A representative set of 87 <i>D. tenuifolia</i> and 3 <i>E. sativa</i> accessions were assessed by 5 SSR and 9 SNP-CAPS markers, allowing a drastic discrimination between both species and the establishment of unequivocal molecular fingerprints for the analyzed accessions. The non-discrimination within six pairs and one trio of <i>D. tenuifolia</i> accessions is discussed. |
| first_indexed | 2024-03-09T15:56:48Z |
| format | Article |
| id | doaj.art-c2f76140084446d096184e50ffe30df3 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T15:56:48Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-c2f76140084446d096184e50ffe30df32023-11-24T17:28:03ZengMDPI AGPlants2223-77472022-12-011124348210.3390/plants11243482Assessment of Wild Rocket (<i>Diplotaxis tenuifolia</i> (L.) DC.) Germplasm Accessions by NGS Identified SSR and SNP MarkersJoão M. Reis0Ricardo J. Pereira1Paula S. Coelho2José M. Leitão3MED—Mediterranean Institute for Agriculture, Environment and Development, Faculdade de Ciências e Tecnologia, Campus de Gambelas, Universidade do Algarve, 8005-139 Faro, PortugalMED—Mediterranean Institute for Agriculture, Environment and Development, Faculdade de Ciências e Tecnologia, Campus de Gambelas, Universidade do Algarve, 8005-139 Faro, PortugalInstituto Nacional de Investigação Agrária e Veterinária, Quinta do Marquês, 2784-505 Oeiras, PortugalMED—Mediterranean Institute for Agriculture, Environment and Development, Faculdade de Ciências e Tecnologia, Campus de Gambelas, Universidade do Algarve, 8005-139 Faro, PortugalRocket is the common designation for two baby-leaf salad crops of the Brassicaceae family: <i>Eruca sativa</i> (L.) Cav., usually referred to as annual garden rocket, and <i>Diplotaxis tenuifolia</i> (L.) DC. commonly named to as perennial wild rocket. <i>E. sativa</i> is used for human consumption since antiquity. However, the growing consumer preference for <i>D. tenuifolia</i> is being accompanied by the fast increase in its production area and commercialization of new cultivars. Nevertheless, the worldwide number of wild rocket accessions maintained in germplasm collections is very reduced, the solution for which situation the project “REMIRucula” intends to contribute, establishing a germplasm collection at the INIAV, Oeiras, Portugal. Herein, we report on the establishment via next generation sequencing (NGS) of the first genome assembly of <i>D. tenuifolia</i> and the identification of specific single sequence repeat (SSR) and single nucleotide polymorphisms (SNP) loci for the establishment of specific DNA-markers for this species. A representative set of 87 <i>D. tenuifolia</i> and 3 <i>E. sativa</i> accessions were assessed by 5 SSR and 9 SNP-CAPS markers, allowing a drastic discrimination between both species and the establishment of unequivocal molecular fingerprints for the analyzed accessions. The non-discrimination within six pairs and one trio of <i>D. tenuifolia</i> accessions is discussed.https://www.mdpi.com/2223-7747/11/24/3482<i>Diplotaxis tenuifolia</i><i>Eruca sativa</i>wild rocketgarden rocketgermplasm collectionsSSR markers |
| spellingShingle | João M. Reis Ricardo J. Pereira Paula S. Coelho José M. Leitão Assessment of Wild Rocket (<i>Diplotaxis tenuifolia</i> (L.) DC.) Germplasm Accessions by NGS Identified SSR and SNP Markers Plants <i>Diplotaxis tenuifolia</i> <i>Eruca sativa</i> wild rocket garden rocket germplasm collections SSR markers |
| title | Assessment of Wild Rocket (<i>Diplotaxis tenuifolia</i> (L.) DC.) Germplasm Accessions by NGS Identified SSR and SNP Markers |
| title_full | Assessment of Wild Rocket (<i>Diplotaxis tenuifolia</i> (L.) DC.) Germplasm Accessions by NGS Identified SSR and SNP Markers |
| title_fullStr | Assessment of Wild Rocket (<i>Diplotaxis tenuifolia</i> (L.) DC.) Germplasm Accessions by NGS Identified SSR and SNP Markers |
| title_full_unstemmed | Assessment of Wild Rocket (<i>Diplotaxis tenuifolia</i> (L.) DC.) Germplasm Accessions by NGS Identified SSR and SNP Markers |
| title_short | Assessment of Wild Rocket (<i>Diplotaxis tenuifolia</i> (L.) DC.) Germplasm Accessions by NGS Identified SSR and SNP Markers |
| title_sort | assessment of wild rocket i diplotaxis tenuifolia i l dc germplasm accessions by ngs identified ssr and snp markers |
| topic | <i>Diplotaxis tenuifolia</i> <i>Eruca sativa</i> wild rocket garden rocket germplasm collections SSR markers |
| url | https://www.mdpi.com/2223-7747/11/24/3482 |
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