A Cluster of Mutations Disrupt the Avirulence but Not the Virulence Function of AvrPto
avrPto in Pseudomonas syringae pv. tomato encodes an avirulence protein that triggers race-specific resistance in tomato plants carrying Pto. The AvrPto protein is secreted from P. syringae pv. tomato to plant cells through the type III secretion pathway and activates race-specific resistance by a d...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2000-06-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2000.13.6.592 |
| _version_ | 1811291228866609152 |
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| author | Libo Shan Ping He Jian-Min Zhou Xiaoyan Tang |
| author_facet | Libo Shan Ping He Jian-Min Zhou Xiaoyan Tang |
| author_sort | Libo Shan |
| collection | DOAJ |
| description | avrPto in Pseudomonas syringae pv. tomato encodes an avirulence protein that triggers race-specific resistance in tomato plants carrying Pto. The AvrPto protein is secreted from P. syringae pv. tomato to plant cells through the type III secretion pathway and activates race-specific resistance by a direct interaction with the Pto protein. Here we report that avrPto enhances the virulence of P. syringae pv. tomato in a strain-dependent manner in tomato plants lacking Pto. To determine whether the virulence function can be structurally separated from the avirulence function, we examined the virulence activity of a group of AvrPto mutants that carry single amino acid substitutions and lack the avirulence activity on tomato plants. Three mutants that were clustered in the center of AvrPto exhibited virulence activity in tomato plants with or without Pto. The rest of the mutations abolished the virulence. The identification of these mutants suggested that the aviru-lence function of AvrPto can be structurally separated from the virulence function. |
| first_indexed | 2024-04-13T04:26:09Z |
| format | Article |
| id | doaj.art-c2f784e0ba5a4b09b1f52067b7be886a |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T04:26:09Z |
| publishDate | 2000-06-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-c2f784e0ba5a4b09b1f52067b7be886a2022-12-22T03:02:31ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062000-06-0113659259810.1094/MPMI.2000.13.6.592A Cluster of Mutations Disrupt the Avirulence but Not the Virulence Function of AvrPtoLibo ShanPing HeJian-Min ZhouXiaoyan TangavrPto in Pseudomonas syringae pv. tomato encodes an avirulence protein that triggers race-specific resistance in tomato plants carrying Pto. The AvrPto protein is secreted from P. syringae pv. tomato to plant cells through the type III secretion pathway and activates race-specific resistance by a direct interaction with the Pto protein. Here we report that avrPto enhances the virulence of P. syringae pv. tomato in a strain-dependent manner in tomato plants lacking Pto. To determine whether the virulence function can be structurally separated from the avirulence function, we examined the virulence activity of a group of AvrPto mutants that carry single amino acid substitutions and lack the avirulence activity on tomato plants. Three mutants that were clustered in the center of AvrPto exhibited virulence activity in tomato plants with or without Pto. The rest of the mutations abolished the virulence. The identification of these mutants suggested that the aviru-lence function of AvrPto can be structurally separated from the virulence function.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2000.13.6.592 |
| spellingShingle | Libo Shan Ping He Jian-Min Zhou Xiaoyan Tang A Cluster of Mutations Disrupt the Avirulence but Not the Virulence Function of AvrPto Molecular Plant-Microbe Interactions |
| title | A Cluster of Mutations Disrupt the Avirulence but Not the Virulence Function of AvrPto |
| title_full | A Cluster of Mutations Disrupt the Avirulence but Not the Virulence Function of AvrPto |
| title_fullStr | A Cluster of Mutations Disrupt the Avirulence but Not the Virulence Function of AvrPto |
| title_full_unstemmed | A Cluster of Mutations Disrupt the Avirulence but Not the Virulence Function of AvrPto |
| title_short | A Cluster of Mutations Disrupt the Avirulence but Not the Virulence Function of AvrPto |
| title_sort | cluster of mutations disrupt the avirulence but not the virulence function of avrpto |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2000.13.6.592 |
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