Familial combined hyperlipidemia plasma stimulates protein secretion by HepG2 cells
The aim of this study was to evaluate whether soluble factors in plasma of familial combined hyperlipidemia (FC333366) patients affect hepatic protein secretion. Cultured human hepatocytes, i.e., HepG2 cells, were incubated with fasting plasma (20%, v/v, in DMEM) from untreated FCHL patients or norm...
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| Format: | Article |
| Language: | English |
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Elsevier
2002-11-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520327590 |
| _version_ | 1818384625252171776 |
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| author | Marleen M.J. van Greevenbroek Vicky M.M-J. Vermeulen Tjerk W.A. de Bruin |
| author_facet | Marleen M.J. van Greevenbroek Vicky M.M-J. Vermeulen Tjerk W.A. de Bruin |
| author_sort | Marleen M.J. van Greevenbroek |
| collection | DOAJ |
| description | The aim of this study was to evaluate whether soluble factors in plasma of familial combined hyperlipidemia (FC333366) patients affect hepatic protein secretion. Cultured human hepatocytes, i.e., HepG2 cells, were incubated with fasting plasma (20%, v/v, in DMEM) from untreated FCHL patients or normolipidemic controls. Overall protein secretion was 10–15% higher after incubation with FCHL plasma. This was specifically caused by an increase in four secreted proteins, with estimated sizes of 240, 180, 120, and <40 kD (P < 0.001, P < 0.006, P < 0.002, P < 0.02, respectively). The 240 kD protein in the secretion proteome was identified as fibronectin by mass spectrometry. Plasma fibronectin concentrations were elevated in FCHL patients, confirming biological relevance of these data. Overall protein secretion by HepG2 cells correlated with concentrations of triglycerides (r = 0.61, P < 0.001) in the applied plasma samples. VLDL+IDL isolated from FCHL patients, induced a higher protein secretion than lipoproteins isolated from controls (P < 0.001). Remarkably, secretion of apoB, the structural protein of VLDL, was stimulated to a similar extent by FCHL and control plasma.FCHL plasma did not induce excess secretion of apoB by HepG2 cells compared with control plasma. FCHL plasma did stimulate secretion of several distinct hepatic proteins, among which fibronectin was identified. |
| first_indexed | 2024-12-14T03:25:14Z |
| format | Article |
| id | doaj.art-c2f7b6094dd5491197903266c7c1cfaa |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-14T03:25:14Z |
| publishDate | 2002-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-c2f7b6094dd5491197903266c7c1cfaa2022-12-21T23:18:54ZengElsevierJournal of Lipid Research0022-22752002-11-01431118461854Familial combined hyperlipidemia plasma stimulates protein secretion by HepG2 cellsMarleen M.J. van Greevenbroek0Vicky M.M-J. Vermeulen1Tjerk W.A. de Bruin2Laboratory for Molecular Metabolism and Endocrinology, Maastricht University, Maastricht, The NetherlandsLaboratory for Molecular Metabolism and Endocrinology, Maastricht University, Maastricht, The NetherlandsLaboratory for Molecular Metabolism and Endocrinology, Maastricht University, Maastricht, The NetherlandsThe aim of this study was to evaluate whether soluble factors in plasma of familial combined hyperlipidemia (FC333366) patients affect hepatic protein secretion. Cultured human hepatocytes, i.e., HepG2 cells, were incubated with fasting plasma (20%, v/v, in DMEM) from untreated FCHL patients or normolipidemic controls. Overall protein secretion was 10–15% higher after incubation with FCHL plasma. This was specifically caused by an increase in four secreted proteins, with estimated sizes of 240, 180, 120, and <40 kD (P < 0.001, P < 0.006, P < 0.002, P < 0.02, respectively). The 240 kD protein in the secretion proteome was identified as fibronectin by mass spectrometry. Plasma fibronectin concentrations were elevated in FCHL patients, confirming biological relevance of these data. Overall protein secretion by HepG2 cells correlated with concentrations of triglycerides (r = 0.61, P < 0.001) in the applied plasma samples. VLDL+IDL isolated from FCHL patients, induced a higher protein secretion than lipoproteins isolated from controls (P < 0.001). Remarkably, secretion of apoB, the structural protein of VLDL, was stimulated to a similar extent by FCHL and control plasma.FCHL plasma did not induce excess secretion of apoB by HepG2 cells compared with control plasma. FCHL plasma did stimulate secretion of several distinct hepatic proteins, among which fibronectin was identified.http://www.sciencedirect.com/science/article/pii/S0022227520327590hepatic lipoproteins fibronectinHepG2familial combined hyperlipidemiatriglycerides |
| spellingShingle | Marleen M.J. van Greevenbroek Vicky M.M-J. Vermeulen Tjerk W.A. de Bruin Familial combined hyperlipidemia plasma stimulates protein secretion by HepG2 cells Journal of Lipid Research hepatic lipoproteins fibronectin HepG2 familial combined hyperlipidemia triglycerides |
| title | Familial combined hyperlipidemia plasma stimulates protein secretion by HepG2 cells |
| title_full | Familial combined hyperlipidemia plasma stimulates protein secretion by HepG2 cells |
| title_fullStr | Familial combined hyperlipidemia plasma stimulates protein secretion by HepG2 cells |
| title_full_unstemmed | Familial combined hyperlipidemia plasma stimulates protein secretion by HepG2 cells |
| title_short | Familial combined hyperlipidemia plasma stimulates protein secretion by HepG2 cells |
| title_sort | familial combined hyperlipidemia plasma stimulates protein secretion by hepg2 cells |
| topic | hepatic lipoproteins fibronectin HepG2 familial combined hyperlipidemia triglycerides |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520327590 |
| work_keys_str_mv | AT marleenmjvangreevenbroek familialcombinedhyperlipidemiaplasmastimulatesproteinsecretionbyhepg2cells AT vickymmjvermeulen familialcombinedhyperlipidemiaplasmastimulatesproteinsecretionbyhepg2cells AT tjerkwadebruin familialcombinedhyperlipidemiaplasmastimulatesproteinsecretionbyhepg2cells |