Plasma proteome changes associated with refractory cytopenia with multilineage dysplasia
<p>Abstract</p> <p>Background</p> <p>Refractory cytopenia with multilineage dysplasia (RCMD) is a subgroup of myelodysplastic syndrome (MDS), which belongs to oncohematological diseases, occurring particularly in elderly patients, and represents a heterogeneous group of...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-10-01
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| Series: | Proteome Science |
| Subjects: | |
| Online Access: | http://www.proteomesci.com/content/9/1/64 |
| _version_ | 1818644722530385920 |
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| author | Čermák Jaroslav Suttnar Jiří Reicheltová Zuzana Májek Pavel Dyr Jan E |
| author_facet | Čermák Jaroslav Suttnar Jiří Reicheltová Zuzana Májek Pavel Dyr Jan E |
| author_sort | Čermák Jaroslav |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Refractory cytopenia with multilineage dysplasia (RCMD) is a subgroup of myelodysplastic syndrome (MDS), which belongs to oncohematological diseases, occurring particularly in elderly patients, and represents a heterogeneous group of bone marrow diseases. The goal of this study was to look for plasma proteins that changed quantitatively or qualitatively in RCMD patients.</p> <p>Results</p> <p>A total of 46 plasma samples were depleted, proteins were separated by 2D SDS-PAGE (pI 4-7), and proteomes were compared using Progenesis SameSpots statistical software. Proteins were identified by nanoLC-MS/MS. Sixty-one unique, significantly (p < 0.05, ANOVA) different spots were found; proteins in 59 spots were successfully identified and corresponded to 57 different proteins. Protein fragmentation was observed in several proteins: complement C4-A, complement C4-B, inter-alpha-trypsin inhibitor heavy chain H4, and endorepellin.</p> <p>Conclusions</p> <p>This study describes proteins, which change quantitatively or qualitatively in RCMD patients, and represents the first report on significant alterations in C4-A and C4-B complement proteins and ITIH4 fragments in patients with MDS-RCMD.</p> |
| first_indexed | 2024-12-17T00:19:22Z |
| format | Article |
| id | doaj.art-c2fe014d92944d5e8dc794ff83921ff2 |
| institution | Directory Open Access Journal |
| issn | 1477-5956 |
| language | English |
| last_indexed | 2024-12-17T00:19:22Z |
| publishDate | 2011-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Proteome Science |
| spelling | doaj.art-c2fe014d92944d5e8dc794ff83921ff22022-12-21T22:10:37ZengBMCProteome Science1477-59562011-10-01916410.1186/1477-5956-9-64Plasma proteome changes associated with refractory cytopenia with multilineage dysplasiaČermák JaroslavSuttnar JiříReicheltová ZuzanaMájek PavelDyr Jan E<p>Abstract</p> <p>Background</p> <p>Refractory cytopenia with multilineage dysplasia (RCMD) is a subgroup of myelodysplastic syndrome (MDS), which belongs to oncohematological diseases, occurring particularly in elderly patients, and represents a heterogeneous group of bone marrow diseases. The goal of this study was to look for plasma proteins that changed quantitatively or qualitatively in RCMD patients.</p> <p>Results</p> <p>A total of 46 plasma samples were depleted, proteins were separated by 2D SDS-PAGE (pI 4-7), and proteomes were compared using Progenesis SameSpots statistical software. Proteins were identified by nanoLC-MS/MS. Sixty-one unique, significantly (p < 0.05, ANOVA) different spots were found; proteins in 59 spots were successfully identified and corresponded to 57 different proteins. Protein fragmentation was observed in several proteins: complement C4-A, complement C4-B, inter-alpha-trypsin inhibitor heavy chain H4, and endorepellin.</p> <p>Conclusions</p> <p>This study describes proteins, which change quantitatively or qualitatively in RCMD patients, and represents the first report on significant alterations in C4-A and C4-B complement proteins and ITIH4 fragments in patients with MDS-RCMD.</p>http://www.proteomesci.com/content/9/1/64myelodysplastic syndromerefractory cytopeniadysplasiaproteome |
| spellingShingle | Čermák Jaroslav Suttnar Jiří Reicheltová Zuzana Májek Pavel Dyr Jan E Plasma proteome changes associated with refractory cytopenia with multilineage dysplasia Proteome Science myelodysplastic syndrome refractory cytopenia dysplasia proteome |
| title | Plasma proteome changes associated with refractory cytopenia with multilineage dysplasia |
| title_full | Plasma proteome changes associated with refractory cytopenia with multilineage dysplasia |
| title_fullStr | Plasma proteome changes associated with refractory cytopenia with multilineage dysplasia |
| title_full_unstemmed | Plasma proteome changes associated with refractory cytopenia with multilineage dysplasia |
| title_short | Plasma proteome changes associated with refractory cytopenia with multilineage dysplasia |
| title_sort | plasma proteome changes associated with refractory cytopenia with multilineage dysplasia |
| topic | myelodysplastic syndrome refractory cytopenia dysplasia proteome |
| url | http://www.proteomesci.com/content/9/1/64 |
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