Comparative transcriptional profiling of two wheat genotypes, with contrasting levels of minerals in grains, shows expression differences during grain filling.
Wheat is one of the most important cereal crops in the world. To identify the candidate genes for mineral accumulation, it is important to examine differential transcriptome between wheat genotypes, with contrasting levels of minerals in grains. A transcriptional comparison of developing grains was...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4218811?pdf=render |
| _version_ | 1818289110169681920 |
|---|---|
| author | Sudhir P Singh Raja Jeet Jitendra Kumar Vishnu Shukla Rakesh Srivastava Shrikant S Mantri Rakesh Tuli |
| author_facet | Sudhir P Singh Raja Jeet Jitendra Kumar Vishnu Shukla Rakesh Srivastava Shrikant S Mantri Rakesh Tuli |
| author_sort | Sudhir P Singh |
| collection | DOAJ |
| description | Wheat is one of the most important cereal crops in the world. To identify the candidate genes for mineral accumulation, it is important to examine differential transcriptome between wheat genotypes, with contrasting levels of minerals in grains. A transcriptional comparison of developing grains was carried out between two wheat genotypes- Triticum aestivum Cv. WL711 (low grain mineral), and T. aestivum L. IITR26 (high grain mineral), using Affymetrix GeneChip Wheat Genome Array. The study identified a total of 580 probe sets as differentially expressed (with log2 fold change of ≥2 at p≤0.01) between the two genotypes, during grain filling. Transcripts with significant differences in induction or repression between the two genotypes included genes related to metal homeostasis, metal tolerance, lignin and flavonoid biosynthesis, amino acid and protein transport, vacuolar-sorting receptor, aquaporins, and stress responses. Meta-analysis revealed spatial and temporal signatures of a majority of the differentially regulated transcripts. |
| first_indexed | 2024-12-13T02:07:04Z |
| format | Article |
| id | doaj.art-c3010f6c22914088878262580ea719ee |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T02:07:04Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c3010f6c22914088878262580ea719ee2022-12-22T00:03:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01911e11171810.1371/journal.pone.0111718Comparative transcriptional profiling of two wheat genotypes, with contrasting levels of minerals in grains, shows expression differences during grain filling.Sudhir P SinghRaja JeetJitendra KumarVishnu ShuklaRakesh SrivastavaShrikant S MantriRakesh TuliWheat is one of the most important cereal crops in the world. To identify the candidate genes for mineral accumulation, it is important to examine differential transcriptome between wheat genotypes, with contrasting levels of minerals in grains. A transcriptional comparison of developing grains was carried out between two wheat genotypes- Triticum aestivum Cv. WL711 (low grain mineral), and T. aestivum L. IITR26 (high grain mineral), using Affymetrix GeneChip Wheat Genome Array. The study identified a total of 580 probe sets as differentially expressed (with log2 fold change of ≥2 at p≤0.01) between the two genotypes, during grain filling. Transcripts with significant differences in induction or repression between the two genotypes included genes related to metal homeostasis, metal tolerance, lignin and flavonoid biosynthesis, amino acid and protein transport, vacuolar-sorting receptor, aquaporins, and stress responses. Meta-analysis revealed spatial and temporal signatures of a majority of the differentially regulated transcripts.http://europepmc.org/articles/PMC4218811?pdf=render |
| spellingShingle | Sudhir P Singh Raja Jeet Jitendra Kumar Vishnu Shukla Rakesh Srivastava Shrikant S Mantri Rakesh Tuli Comparative transcriptional profiling of two wheat genotypes, with contrasting levels of minerals in grains, shows expression differences during grain filling. PLoS ONE |
| title | Comparative transcriptional profiling of two wheat genotypes, with contrasting levels of minerals in grains, shows expression differences during grain filling. |
| title_full | Comparative transcriptional profiling of two wheat genotypes, with contrasting levels of minerals in grains, shows expression differences during grain filling. |
| title_fullStr | Comparative transcriptional profiling of two wheat genotypes, with contrasting levels of minerals in grains, shows expression differences during grain filling. |
| title_full_unstemmed | Comparative transcriptional profiling of two wheat genotypes, with contrasting levels of minerals in grains, shows expression differences during grain filling. |
| title_short | Comparative transcriptional profiling of two wheat genotypes, with contrasting levels of minerals in grains, shows expression differences during grain filling. |
| title_sort | comparative transcriptional profiling of two wheat genotypes with contrasting levels of minerals in grains shows expression differences during grain filling |
| url | http://europepmc.org/articles/PMC4218811?pdf=render |
| work_keys_str_mv | AT sudhirpsingh comparativetranscriptionalprofilingoftwowheatgenotypeswithcontrastinglevelsofmineralsingrainsshowsexpressiondifferencesduringgrainfilling AT rajajeet comparativetranscriptionalprofilingoftwowheatgenotypeswithcontrastinglevelsofmineralsingrainsshowsexpressiondifferencesduringgrainfilling AT jitendrakumar comparativetranscriptionalprofilingoftwowheatgenotypeswithcontrastinglevelsofmineralsingrainsshowsexpressiondifferencesduringgrainfilling AT vishnushukla comparativetranscriptionalprofilingoftwowheatgenotypeswithcontrastinglevelsofmineralsingrainsshowsexpressiondifferencesduringgrainfilling AT rakeshsrivastava comparativetranscriptionalprofilingoftwowheatgenotypeswithcontrastinglevelsofmineralsingrainsshowsexpressiondifferencesduringgrainfilling AT shrikantsmantri comparativetranscriptionalprofilingoftwowheatgenotypeswithcontrastinglevelsofmineralsingrainsshowsexpressiondifferencesduringgrainfilling AT rakeshtuli comparativetranscriptionalprofilingoftwowheatgenotypeswithcontrastinglevelsofmineralsingrainsshowsexpressiondifferencesduringgrainfilling |