Layered double hydroxide nanoparticles as an appealing nanoparticle in gene/plasmid and drug delivery system in C2C12 myoblast cells
Gene and drug delivery systems need crucial update in the issue of nanocarriers. Layered double hydroxides (LDHs) are known as biocompatible inorganic lamellar nanomaterials with versatile properties. In the present study, Zn/Al-LDH nanoparticle was synthesized and characterized by FTIR, XRD, SEM, T...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2018.1559182 |
| _version_ | 1817981399096885248 |
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| author | Parivar Yazdani Elham Mansouri Shirin Eyvazi Vahid Yousefi Homan Kahroba Mohammad Saeid Hejazi Asghar Mesbahi Vahideh Tarhriz Mir Mahdi Abolghasemi |
| author_facet | Parivar Yazdani Elham Mansouri Shirin Eyvazi Vahid Yousefi Homan Kahroba Mohammad Saeid Hejazi Asghar Mesbahi Vahideh Tarhriz Mir Mahdi Abolghasemi |
| author_sort | Parivar Yazdani |
| collection | DOAJ |
| description | Gene and drug delivery systems need crucial update in the issue of nanocarriers. Layered double hydroxides (LDHs) are known as biocompatible inorganic lamellar nanomaterials with versatile properties. In the present study, Zn/Al-LDH nanoparticle was synthesized and characterized by FTIR, XRD, SEM, TEM and Zeta potential tests and then intercalated with valproate and methyldopa by co-precipitation and ion exchange methods. These nanocarriers were applied as high activity nanolayers-based delivery systems. On the other hand, Zn/Al-LDH + plasmid/gene (pCEP4/Cdk9) evaluated on C2C12 myoblast cells. Co-operation loading indicated high efficiency of sorting and release of drugs. Additionally, the Real-Time PCR and Western blotting results for plasmid-gene (pCEP4/Cdk9) delivery showed that Zn/Al-LDH nanoparticles can be used as an effective carrier in cellular uptake and release of genes for gene therapy. Easy and cost-effective production of Zn/Al-LDH nanoparticles proposed them as potential alternatives for the traditional routs of drug/gene delivery. |
| first_indexed | 2024-04-13T23:06:36Z |
| format | Article |
| id | doaj.art-c303338e29c34a429e37855074afeee0 |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-04-13T23:06:36Z |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-c303338e29c34a429e37855074afeee02022-12-22T02:25:41ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2019-12-0147143644210.1080/21691401.2018.1559182Layered double hydroxide nanoparticles as an appealing nanoparticle in gene/plasmid and drug delivery system in C2C12 myoblast cellsParivar Yazdani0Elham Mansouri1Shirin Eyvazi2Vahid Yousefi3Homan Kahroba4Mohammad Saeid Hejazi5Asghar Mesbahi6Vahideh Tarhriz7Mir Mahdi Abolghasemi8Molecular Medicine Research Center, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, IranMolecular Medicine Research Center, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranMolecular Medicine Research Center, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Molecular Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, IranMolecular Medicine Research Center, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, IranMolecular Medicine Research Center, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, IranMolecular Medicine Research Center, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Chemistry, Faculty of Science, University of Maragheh, Maragheh, IranGene and drug delivery systems need crucial update in the issue of nanocarriers. Layered double hydroxides (LDHs) are known as biocompatible inorganic lamellar nanomaterials with versatile properties. In the present study, Zn/Al-LDH nanoparticle was synthesized and characterized by FTIR, XRD, SEM, TEM and Zeta potential tests and then intercalated with valproate and methyldopa by co-precipitation and ion exchange methods. These nanocarriers were applied as high activity nanolayers-based delivery systems. On the other hand, Zn/Al-LDH + plasmid/gene (pCEP4/Cdk9) evaluated on C2C12 myoblast cells. Co-operation loading indicated high efficiency of sorting and release of drugs. Additionally, the Real-Time PCR and Western blotting results for plasmid-gene (pCEP4/Cdk9) delivery showed that Zn/Al-LDH nanoparticles can be used as an effective carrier in cellular uptake and release of genes for gene therapy. Easy and cost-effective production of Zn/Al-LDH nanoparticles proposed them as potential alternatives for the traditional routs of drug/gene delivery.https://www.tandfonline.com/doi/10.1080/21691401.2018.1559182Layered double hydroxidenanomaterialdrug/gene deliverypCEP4/Cdk9 |
| spellingShingle | Parivar Yazdani Elham Mansouri Shirin Eyvazi Vahid Yousefi Homan Kahroba Mohammad Saeid Hejazi Asghar Mesbahi Vahideh Tarhriz Mir Mahdi Abolghasemi Layered double hydroxide nanoparticles as an appealing nanoparticle in gene/plasmid and drug delivery system in C2C12 myoblast cells Artificial Cells, Nanomedicine, and Biotechnology Layered double hydroxide nanomaterial drug/gene delivery pCEP4/Cdk9 |
| title | Layered double hydroxide nanoparticles as an appealing nanoparticle in gene/plasmid and drug delivery system in C2C12 myoblast cells |
| title_full | Layered double hydroxide nanoparticles as an appealing nanoparticle in gene/plasmid and drug delivery system in C2C12 myoblast cells |
| title_fullStr | Layered double hydroxide nanoparticles as an appealing nanoparticle in gene/plasmid and drug delivery system in C2C12 myoblast cells |
| title_full_unstemmed | Layered double hydroxide nanoparticles as an appealing nanoparticle in gene/plasmid and drug delivery system in C2C12 myoblast cells |
| title_short | Layered double hydroxide nanoparticles as an appealing nanoparticle in gene/plasmid and drug delivery system in C2C12 myoblast cells |
| title_sort | layered double hydroxide nanoparticles as an appealing nanoparticle in gene plasmid and drug delivery system in c2c12 myoblast cells |
| topic | Layered double hydroxide nanomaterial drug/gene delivery pCEP4/Cdk9 |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2018.1559182 |
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