Multivariate analysis using high definition flow cytometry reveals distinct T cell repertoires between the foetal-maternal interface and the peripheral blood
The human T-cell compartment is a complex system and while some information is known on repertoire composition and dynamics in the peripheral blood, little is known on repertoire composition at different anatomical sites. Here, we determine the T-cell receptor β-variable (TRBV) repertoire at the dec...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2014-02-01
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| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fimmu.2014.00033/full |
| _version_ | 1819074805654093824 |
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| author | Michelle eNeller Brigittie eSantner-Nanan Rebekah eBrennan Peter eHsu Steven eJoung Ralph eNanan Scott eBurrows John J Miles |
| author_facet | Michelle eNeller Brigittie eSantner-Nanan Rebekah eBrennan Peter eHsu Steven eJoung Ralph eNanan Scott eBurrows John J Miles |
| author_sort | Michelle eNeller |
| collection | DOAJ |
| description | The human T-cell compartment is a complex system and while some information is known on repertoire composition and dynamics in the peripheral blood, little is known on repertoire composition at different anatomical sites. Here, we determine the T-cell receptor β-variable (TRBV) repertoire at the decidua and compare it with the peripheral blood during normal pregnancy and preclampsia. We found total T-cell subset disparity of up to 58% between sites, including large signature TRBV expansions unique to the foetal-maternal interface. Defining the functional nature and specificity of compartment-specific T-cells will be necessary if we are to understand localised immunity, tolerance and pathogenesis. |
| first_indexed | 2024-12-21T18:15:21Z |
| format | Article |
| id | doaj.art-c30579aed92145e89b22d382be801902 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-21T18:15:21Z |
| publishDate | 2014-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-c30579aed92145e89b22d382be8019022022-12-21T18:54:40ZengFrontiers Media S.A.Frontiers in Immunology1664-32242014-02-01510.3389/fimmu.2014.0003376688Multivariate analysis using high definition flow cytometry reveals distinct T cell repertoires between the foetal-maternal interface and the peripheral bloodMichelle eNeller0Brigittie eSantner-Nanan1Rebekah eBrennan2Peter eHsu3Steven eJoung4Ralph eNanan5Scott eBurrows6John J Miles7QIMR Berghofer Medical Research InstituteSydney Medical School, The University of SydneyQIMR Berghofer Medical Research InstituteSydney Medical School, The University of SydneySydney Medical School, The University of SydneySydney Medical School, The University of SydneyQIMR Berghofer Medical Research InstituteQIMR Berghofer Medical Research InstituteThe human T-cell compartment is a complex system and while some information is known on repertoire composition and dynamics in the peripheral blood, little is known on repertoire composition at different anatomical sites. Here, we determine the T-cell receptor β-variable (TRBV) repertoire at the decidua and compare it with the peripheral blood during normal pregnancy and preclampsia. We found total T-cell subset disparity of up to 58% between sites, including large signature TRBV expansions unique to the foetal-maternal interface. Defining the functional nature and specificity of compartment-specific T-cells will be necessary if we are to understand localised immunity, tolerance and pathogenesis.http://journal.frontiersin.org/Journal/10.3389/fimmu.2014.00033/fullPlacentaCD4+ T cellsCD8+ T cellsT cell receptor (TCR)Treg cellsPeripheral Blood |
| spellingShingle | Michelle eNeller Brigittie eSantner-Nanan Rebekah eBrennan Peter eHsu Steven eJoung Ralph eNanan Scott eBurrows John J Miles Multivariate analysis using high definition flow cytometry reveals distinct T cell repertoires between the foetal-maternal interface and the peripheral blood Frontiers in Immunology Placenta CD4+ T cells CD8+ T cells T cell receptor (TCR) Treg cells Peripheral Blood |
| title | Multivariate analysis using high definition flow cytometry reveals distinct T cell repertoires between the foetal-maternal interface and the peripheral blood |
| title_full | Multivariate analysis using high definition flow cytometry reveals distinct T cell repertoires between the foetal-maternal interface and the peripheral blood |
| title_fullStr | Multivariate analysis using high definition flow cytometry reveals distinct T cell repertoires between the foetal-maternal interface and the peripheral blood |
| title_full_unstemmed | Multivariate analysis using high definition flow cytometry reveals distinct T cell repertoires between the foetal-maternal interface and the peripheral blood |
| title_short | Multivariate analysis using high definition flow cytometry reveals distinct T cell repertoires between the foetal-maternal interface and the peripheral blood |
| title_sort | multivariate analysis using high definition flow cytometry reveals distinct t cell repertoires between the foetal maternal interface and the peripheral blood |
| topic | Placenta CD4+ T cells CD8+ T cells T cell receptor (TCR) Treg cells Peripheral Blood |
| url | http://journal.frontiersin.org/Journal/10.3389/fimmu.2014.00033/full |
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