Mechanical and Degradation Properties of Hybrid Scaffolds for Tissue Engineered Heart Valve (TEHV)
In addition to biocompatibility, an ideal scaffold for the regeneration of valvular tissue should also replicate the natural heart valve extracellular matrix (ECM) in terms of biomechanical properties and structural stability. In our previous paper, we demonstrated the development of collagen type I...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-03-01
|
| Series: | Journal of Functional Biomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4983/12/1/20 |
| _version_ | 1797542139997978624 |
|---|---|
| author | Rabia Nazir Arne Bruyneel Carolyn Carr Jan Czernuszka |
| author_facet | Rabia Nazir Arne Bruyneel Carolyn Carr Jan Czernuszka |
| author_sort | Rabia Nazir |
| collection | DOAJ |
| description | In addition to biocompatibility, an ideal scaffold for the regeneration of valvular tissue should also replicate the natural heart valve extracellular matrix (ECM) in terms of biomechanical properties and structural stability. In our previous paper, we demonstrated the development of collagen type I and hyaluronic acid (HA)-based scaffolds with interlaced microstructure. Such hybrid scaffolds were found to be compatible with cardiosphere-derived cells (CDCs) to potentially regenerate the diseased aortic heart valve. This paper focused on the quantification of the effect of crosslinking density on the mechanical properties under dry and wet conditions as well as degradation resistance. Elastic moduli increased with increasing crosslinking densities, in the dry and wet state, for parent networks, whereas those of interlaced scaffolds were higher than either network alone. Compressive and storage moduli ranged from 35 ± 5 to 95 ± 5 kPa and 16 ± 2 kPa to 113 ± 6 kPa, respectively, in the dry state. Storage moduli, in the dry state, matched and exceeded those of human aortic valve leaflets (HAVL). Similarly, degradation resistance increased with increasing the crosslinking densities for collagen-only and HA-only scaffolds. Interlaced scaffolds showed partial degradation in the presence of either collagenase or hyaluronidase as compared to when exposed to both enzymes together. These results agree with our previous findings that interlaced scaffolds were composed of independent collagen and HA networks without crosslinking between them. Thus, collagen/HA interlaced scaffolds have the potential to fill in the niche for designing an ideal tissue engineered heart valve (TEHV). |
| first_indexed | 2024-03-10T13:25:29Z |
| format | Article |
| id | doaj.art-c305947fef1d4fce9142ef269e375e68 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-10T13:25:29Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-c305947fef1d4fce9142ef269e375e682023-11-21T09:42:45ZengMDPI AGJournal of Functional Biomaterials2079-49832021-03-011212010.3390/jfb12010020Mechanical and Degradation Properties of Hybrid Scaffolds for Tissue Engineered Heart Valve (TEHV)Rabia Nazir0Arne Bruyneel1Carolyn Carr2Jan Czernuszka3Department of Materials, University of Oxford, Parks Road, Oxford OX1 3PH, UKDepartment of Physiology, Anatomy and Genetics, University of Oxford, Parks Road, Oxford OX1 3PT, UKDepartment of Physiology, Anatomy and Genetics, University of Oxford, Parks Road, Oxford OX1 3PT, UKDepartment of Materials, University of Oxford, Parks Road, Oxford OX1 3PH, UKIn addition to biocompatibility, an ideal scaffold for the regeneration of valvular tissue should also replicate the natural heart valve extracellular matrix (ECM) in terms of biomechanical properties and structural stability. In our previous paper, we demonstrated the development of collagen type I and hyaluronic acid (HA)-based scaffolds with interlaced microstructure. Such hybrid scaffolds were found to be compatible with cardiosphere-derived cells (CDCs) to potentially regenerate the diseased aortic heart valve. This paper focused on the quantification of the effect of crosslinking density on the mechanical properties under dry and wet conditions as well as degradation resistance. Elastic moduli increased with increasing crosslinking densities, in the dry and wet state, for parent networks, whereas those of interlaced scaffolds were higher than either network alone. Compressive and storage moduli ranged from 35 ± 5 to 95 ± 5 kPa and 16 ± 2 kPa to 113 ± 6 kPa, respectively, in the dry state. Storage moduli, in the dry state, matched and exceeded those of human aortic valve leaflets (HAVL). Similarly, degradation resistance increased with increasing the crosslinking densities for collagen-only and HA-only scaffolds. Interlaced scaffolds showed partial degradation in the presence of either collagenase or hyaluronidase as compared to when exposed to both enzymes together. These results agree with our previous findings that interlaced scaffolds were composed of independent collagen and HA networks without crosslinking between them. Thus, collagen/HA interlaced scaffolds have the potential to fill in the niche for designing an ideal tissue engineered heart valve (TEHV).https://www.mdpi.com/2079-4983/12/1/20compressive modulusdynamic mechanical propertiesenzymatic degradationcrosslinking densityaortic valve repair |
| spellingShingle | Rabia Nazir Arne Bruyneel Carolyn Carr Jan Czernuszka Mechanical and Degradation Properties of Hybrid Scaffolds for Tissue Engineered Heart Valve (TEHV) Journal of Functional Biomaterials compressive modulus dynamic mechanical properties enzymatic degradation crosslinking density aortic valve repair |
| title | Mechanical and Degradation Properties of Hybrid Scaffolds for Tissue Engineered Heart Valve (TEHV) |
| title_full | Mechanical and Degradation Properties of Hybrid Scaffolds for Tissue Engineered Heart Valve (TEHV) |
| title_fullStr | Mechanical and Degradation Properties of Hybrid Scaffolds for Tissue Engineered Heart Valve (TEHV) |
| title_full_unstemmed | Mechanical and Degradation Properties of Hybrid Scaffolds for Tissue Engineered Heart Valve (TEHV) |
| title_short | Mechanical and Degradation Properties of Hybrid Scaffolds for Tissue Engineered Heart Valve (TEHV) |
| title_sort | mechanical and degradation properties of hybrid scaffolds for tissue engineered heart valve tehv |
| topic | compressive modulus dynamic mechanical properties enzymatic degradation crosslinking density aortic valve repair |
| url | https://www.mdpi.com/2079-4983/12/1/20 |
| work_keys_str_mv | AT rabianazir mechanicalanddegradationpropertiesofhybridscaffoldsfortissueengineeredheartvalvetehv AT arnebruyneel mechanicalanddegradationpropertiesofhybridscaffoldsfortissueengineeredheartvalvetehv AT carolyncarr mechanicalanddegradationpropertiesofhybridscaffoldsfortissueengineeredheartvalvetehv AT janczernuszka mechanicalanddegradationpropertiesofhybridscaffoldsfortissueengineeredheartvalvetehv |