Glucoraphanin Triggers Rapid Antidepressant Responses in a Rat Model of Beta Amyloid-Induced Depressive-like Behaviour
Glucoraphanin (GRA) is a natural compound that has shown beneficial effects in chronic diseases and in central nervous system disorders. Moreover, GRA displayed antidepressant activity in preclinical models. We have previously demonstrated that a single intracerebroventricular administration of solu...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-08-01
|
| Series: | Pharmaceuticals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8247/15/9/1054 |
| _version_ | 1797483822039695360 |
|---|---|
| author | Paolo Tucci Maria Bove Vladyslav Sikora Stefania Dimonte Maria Grazia Morgese Stefania Schiavone Lorenzo Di Cesare Mannelli Carla Ghelardini Luigia Trabace |
| author_facet | Paolo Tucci Maria Bove Vladyslav Sikora Stefania Dimonte Maria Grazia Morgese Stefania Schiavone Lorenzo Di Cesare Mannelli Carla Ghelardini Luigia Trabace |
| author_sort | Paolo Tucci |
| collection | DOAJ |
| description | Glucoraphanin (GRA) is a natural compound that has shown beneficial effects in chronic diseases and in central nervous system disorders. Moreover, GRA displayed antidepressant activity in preclinical models. We have previously demonstrated that a single intracerebroventricular administration of soluble amyloid-beta 1-42 (sAβ 1-42) in rat evokes a depressive-like phenotype by increasing immobility frequency in the forced swimming test (FST). The aim of this work was to investigate the effect of GRA in naïve and in sAβ-1-42-treated rats by using the FST. Behavioural analyses were accompanied by neurochemical and biochemical measurements in the prefrontal cortex (PFC), such as serotonin (5-HT), noradrenaline (NA), kynurenine (KYN), tryptophan (TRP), reactive oxygen species (ROS) and the transcription nuclear factor kappa B (NF-kB) levels. We reported that GRA administration in naïve rats at the dose of 50 mg/kg reduced the immobility frequency in the FST and increased 5-HT and NA levels in the PFC compared to controls. At the same dose, GRA reverted depressive-like effects of sAβ 1-42 administration, restored the 5-HT levels and reduced NF-kB, KYN and ROS levels in PFC. In conclusion, GRA rapidly reverting depressive-like behaviour, together with biochemical and neurochemical alterations, might represent a safe and natural candidate for the treatment of depression. |
| first_indexed | 2024-03-09T22:52:36Z |
| format | Article |
| id | doaj.art-c30c9addf87549abaebaa705b7985fe6 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-09T22:52:36Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-c30c9addf87549abaebaa705b7985fe62023-11-23T18:17:43ZengMDPI AGPharmaceuticals1424-82472022-08-01159105410.3390/ph15091054Glucoraphanin Triggers Rapid Antidepressant Responses in a Rat Model of Beta Amyloid-Induced Depressive-like BehaviourPaolo Tucci0Maria Bove1Vladyslav Sikora2Stefania Dimonte3Maria Grazia Morgese4Stefania Schiavone5Lorenzo Di Cesare Mannelli6Carla Ghelardini7Luigia Trabace8Department of Clinical and Experimental Medicine, University of Foggia, 71122 Foggia, ItalyDepartment of Clinical and Experimental Medicine, University of Foggia, 71122 Foggia, ItalyDepartment of Clinical and Experimental Medicine, University of Foggia, 71122 Foggia, ItalyDepartment of Clinical and Experimental Medicine, University of Foggia, 71122 Foggia, ItalyDepartment of Clinical and Experimental Medicine, University of Foggia, 71122 Foggia, ItalyDepartment of Clinical and Experimental Medicine, University of Foggia, 71122 Foggia, ItalyPharmacology and Toxicology Section, Department of Neuroscience, Psychology, Drug Research and Child Health (NEUROFARBA), University of Firenze, 50139 Firenze, ItalyPharmacology and Toxicology Section, Department of Neuroscience, Psychology, Drug Research and Child Health (NEUROFARBA), University of Firenze, 50139 Firenze, ItalyDepartment of Clinical and Experimental Medicine, University of Foggia, 71122 Foggia, ItalyGlucoraphanin (GRA) is a natural compound that has shown beneficial effects in chronic diseases and in central nervous system disorders. Moreover, GRA displayed antidepressant activity in preclinical models. We have previously demonstrated that a single intracerebroventricular administration of soluble amyloid-beta 1-42 (sAβ 1-42) in rat evokes a depressive-like phenotype by increasing immobility frequency in the forced swimming test (FST). The aim of this work was to investigate the effect of GRA in naïve and in sAβ-1-42-treated rats by using the FST. Behavioural analyses were accompanied by neurochemical and biochemical measurements in the prefrontal cortex (PFC), such as serotonin (5-HT), noradrenaline (NA), kynurenine (KYN), tryptophan (TRP), reactive oxygen species (ROS) and the transcription nuclear factor kappa B (NF-kB) levels. We reported that GRA administration in naïve rats at the dose of 50 mg/kg reduced the immobility frequency in the FST and increased 5-HT and NA levels in the PFC compared to controls. At the same dose, GRA reverted depressive-like effects of sAβ 1-42 administration, restored the 5-HT levels and reduced NF-kB, KYN and ROS levels in PFC. In conclusion, GRA rapidly reverting depressive-like behaviour, together with biochemical and neurochemical alterations, might represent a safe and natural candidate for the treatment of depression.https://www.mdpi.com/1424-8247/15/9/1054glucoraphaninantidepressantamyloid beta |
| spellingShingle | Paolo Tucci Maria Bove Vladyslav Sikora Stefania Dimonte Maria Grazia Morgese Stefania Schiavone Lorenzo Di Cesare Mannelli Carla Ghelardini Luigia Trabace Glucoraphanin Triggers Rapid Antidepressant Responses in a Rat Model of Beta Amyloid-Induced Depressive-like Behaviour Pharmaceuticals glucoraphanin antidepressant amyloid beta |
| title | Glucoraphanin Triggers Rapid Antidepressant Responses in a Rat Model of Beta Amyloid-Induced Depressive-like Behaviour |
| title_full | Glucoraphanin Triggers Rapid Antidepressant Responses in a Rat Model of Beta Amyloid-Induced Depressive-like Behaviour |
| title_fullStr | Glucoraphanin Triggers Rapid Antidepressant Responses in a Rat Model of Beta Amyloid-Induced Depressive-like Behaviour |
| title_full_unstemmed | Glucoraphanin Triggers Rapid Antidepressant Responses in a Rat Model of Beta Amyloid-Induced Depressive-like Behaviour |
| title_short | Glucoraphanin Triggers Rapid Antidepressant Responses in a Rat Model of Beta Amyloid-Induced Depressive-like Behaviour |
| title_sort | glucoraphanin triggers rapid antidepressant responses in a rat model of beta amyloid induced depressive like behaviour |
| topic | glucoraphanin antidepressant amyloid beta |
| url | https://www.mdpi.com/1424-8247/15/9/1054 |
| work_keys_str_mv | AT paolotucci glucoraphanintriggersrapidantidepressantresponsesinaratmodelofbetaamyloidinduceddepressivelikebehaviour AT mariabove glucoraphanintriggersrapidantidepressantresponsesinaratmodelofbetaamyloidinduceddepressivelikebehaviour AT vladyslavsikora glucoraphanintriggersrapidantidepressantresponsesinaratmodelofbetaamyloidinduceddepressivelikebehaviour AT stefaniadimonte glucoraphanintriggersrapidantidepressantresponsesinaratmodelofbetaamyloidinduceddepressivelikebehaviour AT mariagraziamorgese glucoraphanintriggersrapidantidepressantresponsesinaratmodelofbetaamyloidinduceddepressivelikebehaviour AT stefaniaschiavone glucoraphanintriggersrapidantidepressantresponsesinaratmodelofbetaamyloidinduceddepressivelikebehaviour AT lorenzodicesaremannelli glucoraphanintriggersrapidantidepressantresponsesinaratmodelofbetaamyloidinduceddepressivelikebehaviour AT carlaghelardini glucoraphanintriggersrapidantidepressantresponsesinaratmodelofbetaamyloidinduceddepressivelikebehaviour AT luigiatrabace glucoraphanintriggersrapidantidepressantresponsesinaratmodelofbetaamyloidinduceddepressivelikebehaviour |