Genetic Relationships of Pistacia Species and Cultivars by SCoT Markers
ObjectivePistachio is one of the most important agricultural products and iran has the richest germplasm of pistachio in the world. The presence of this genetic resources will be an appropriate opportunity for use in breeding purposes. Knowledge of genetic relationships among pistachio genotypes has...
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Shahid Bahonar University of Kerman
2022-12-01
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| Series: | مجله بیوتکنولوژی کشاورزی |
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| Online Access: | https://jab.uk.ac.ir/article_3510_b6a3d104eeba376cc32072c04201c83c.pdf |
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| author | Mohsen Saadlou Parizi Sodabeh Jahanbakhsh Godehkahriz Hosein Dashti Roohallah Saberi Riseh Hojjat Hashemi Nasab |
| author_facet | Mohsen Saadlou Parizi Sodabeh Jahanbakhsh Godehkahriz Hosein Dashti Roohallah Saberi Riseh Hojjat Hashemi Nasab |
| author_sort | Mohsen Saadlou Parizi |
| collection | DOAJ |
| description | ObjectivePistachio is one of the most important agricultural products and iran has the richest germplasm of pistachio in the world. The presence of this genetic resources will be an appropriate opportunity for use in breeding purposes. Knowledge of genetic relationships among pistachio genotypes has important role in it’s breeding programs. Molecular markers are one of the powerful tools for studying plant phylogenetic relationships. Start Codon Targeted (SCoT) technique is one of the molecular systems that used to assess the genetic relationship among different plant species and cultivars. This study was performed in order to evaluate the genetic relationships between a numbers of Pistacia species and cultivars using SCoT molecular markers and to assess the usefulness of this markers in differentiating this genus.Materials and methodsPlant materials of this study are included 29 genotypes of domestic and wild species of genus Pistacia. A total of 25 SCoT primers were used to evaluate the genetic relationships. Genomic DNA were extracted from leaf samples using CTAB method with minor modifications. The quantity and quality of the extracted DNA were measured by spectrophotometer and agarose gel electrophoresis. Cluster analysis based on Jaccard’s similarity matrix and complete linkage algorithm and Principal coordinate analysis were performed using NTSYSpc 2.02e software.ResultsIn total, 449 DNA fragments were amplified by primers out of which 433 bands (96/43%) were polymorphic. The average number of amplified fragments for each primer was 17.96 bands with a mean of 17.32 polymorphic bands per primer. A number of species-spicific marker were detected in some Genotypes. The average of polymorphism information content values varied from 0/18 to 0/38. Also, the values of marker indices ranged from 0/56 to 4/36. The range of similarity coefficients of genotypes varied between 25% to 68%. Cluster analysis divided Genotypes into two main cluster including vera (domestic) and wild species. Principal coordinate analysis separated vera cultivars and genotypes from wild species and confirmed the results of cluster analysis.ConclusionsThe results of this study demonstrated that SCoT molecular markers detected high polymorphism among pistachio species and cultivars and differentiated the studid genotypes. Therefore, SCoT Marker is a useful tool for studying phylogenetic relationships in genus Pistacia. |
| first_indexed | 2024-03-08T22:49:59Z |
| format | Article |
| id | doaj.art-c317b74824cc4ed997d84449437ddcd1 |
| institution | Directory Open Access Journal |
| issn | 2228-6705 2228-6500 |
| language | fas |
| last_indexed | 2024-03-08T22:49:59Z |
| publishDate | 2022-12-01 |
| publisher | Shahid Bahonar University of Kerman |
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| series | مجله بیوتکنولوژی کشاورزی |
| spelling | doaj.art-c317b74824cc4ed997d84449437ddcd12023-12-16T17:42:01ZfasShahid Bahonar University of Kermanمجله بیوتکنولوژی کشاورزی2228-67052228-65002022-12-0114412010.22103/jab.2022.18817.13743510Genetic Relationships of Pistacia Species and Cultivars by SCoT MarkersMohsen Saadlou Parizi0Sodabeh Jahanbakhsh Godehkahriz1Hosein Dashti2Roohallah Saberi Riseh3Hojjat Hashemi Nasab4Ph.D. Student, Department of Production Engineering and Breeding Genetics, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.Department of plant genetics and production engineering, Faculty of agriculture and natural resources, University of Mohaghegh Ardabili, Ardabil, IranProfessor, Department of Genetics and Plant Production, Faculty of Agriculture, Valiasr University, Rafsanjan, Rafsanjan, Iran. emailProfessor, Department of Plant Protection, Faculty of Agriculture, Valiasr University, Rafsanjan, Rafsanjan, Iran.Research Assistant Professor, Pistachio Research Center, Horticultural sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rafsanjan, IranObjectivePistachio is one of the most important agricultural products and iran has the richest germplasm of pistachio in the world. The presence of this genetic resources will be an appropriate opportunity for use in breeding purposes. Knowledge of genetic relationships among pistachio genotypes has important role in it’s breeding programs. Molecular markers are one of the powerful tools for studying plant phylogenetic relationships. Start Codon Targeted (SCoT) technique is one of the molecular systems that used to assess the genetic relationship among different plant species and cultivars. This study was performed in order to evaluate the genetic relationships between a numbers of Pistacia species and cultivars using SCoT molecular markers and to assess the usefulness of this markers in differentiating this genus.Materials and methodsPlant materials of this study are included 29 genotypes of domestic and wild species of genus Pistacia. A total of 25 SCoT primers were used to evaluate the genetic relationships. Genomic DNA were extracted from leaf samples using CTAB method with minor modifications. The quantity and quality of the extracted DNA were measured by spectrophotometer and agarose gel electrophoresis. Cluster analysis based on Jaccard’s similarity matrix and complete linkage algorithm and Principal coordinate analysis were performed using NTSYSpc 2.02e software.ResultsIn total, 449 DNA fragments were amplified by primers out of which 433 bands (96/43%) were polymorphic. The average number of amplified fragments for each primer was 17.96 bands with a mean of 17.32 polymorphic bands per primer. A number of species-spicific marker were detected in some Genotypes. The average of polymorphism information content values varied from 0/18 to 0/38. Also, the values of marker indices ranged from 0/56 to 4/36. The range of similarity coefficients of genotypes varied between 25% to 68%. Cluster analysis divided Genotypes into two main cluster including vera (domestic) and wild species. Principal coordinate analysis separated vera cultivars and genotypes from wild species and confirmed the results of cluster analysis.ConclusionsThe results of this study demonstrated that SCoT molecular markers detected high polymorphism among pistachio species and cultivars and differentiated the studid genotypes. Therefore, SCoT Marker is a useful tool for studying phylogenetic relationships in genus Pistacia.https://jab.uk.ac.ir/article_3510_b6a3d104eeba376cc32072c04201c83c.pdfcluster analysisphylogeneticpistachiomolecular markerscot marker |
| spellingShingle | Mohsen Saadlou Parizi Sodabeh Jahanbakhsh Godehkahriz Hosein Dashti Roohallah Saberi Riseh Hojjat Hashemi Nasab Genetic Relationships of Pistacia Species and Cultivars by SCoT Markers مجله بیوتکنولوژی کشاورزی cluster analysis phylogenetic pistachio molecular marker scot marker |
| title | Genetic Relationships of Pistacia Species and Cultivars by SCoT Markers |
| title_full | Genetic Relationships of Pistacia Species and Cultivars by SCoT Markers |
| title_fullStr | Genetic Relationships of Pistacia Species and Cultivars by SCoT Markers |
| title_full_unstemmed | Genetic Relationships of Pistacia Species and Cultivars by SCoT Markers |
| title_short | Genetic Relationships of Pistacia Species and Cultivars by SCoT Markers |
| title_sort | genetic relationships of pistacia species and cultivars by scot markers |
| topic | cluster analysis phylogenetic pistachio molecular marker scot marker |
| url | https://jab.uk.ac.ir/article_3510_b6a3d104eeba376cc32072c04201c83c.pdf |
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