Supragingival mycobiome and inter-kingdom interactions in dental caries
Background: Recent studies have reveled the presence of a complex fungal community (mycobiome) in the oral cavity. However, the role of oral mycobiome in dental caries and its interaction with caries-associated bacteria is not yet clear. Methods: Whole-mouth supragingival plaque samples from 30 chil...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Journal of Oral Microbiology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/20002297.2020.1729305 |
| _version_ | 1831683983999500288 |
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| author | Divyashri Baraniya Tsute Chen Anubhav Nahar Fadhl Alakwaa Jennifer Hill Marisol Tellez Amid Ismail Sumant Puri Nezar Noor Al-Hebshi |
| author_facet | Divyashri Baraniya Tsute Chen Anubhav Nahar Fadhl Alakwaa Jennifer Hill Marisol Tellez Amid Ismail Sumant Puri Nezar Noor Al-Hebshi |
| author_sort | Divyashri Baraniya |
| collection | DOAJ |
| description | Background: Recent studies have reveled the presence of a complex fungal community (mycobiome) in the oral cavity. However, the role of oral mycobiome in dental caries and its interaction with caries-associated bacteria is not yet clear. Methods: Whole-mouth supragingival plaque samples from 30 children (6–10 years old) with no caries, early caries, or advanced caries were sequenced for internal transcribed spacer 2 (ITS-2). The mycobiome profiles were correlated with previously published bacteriome counterparts. Interaction among selected fungal and bacterial species was assessed by co-culture or spent media experiments. Results: Fungal load was extremely low. Candida, Malassezia, Cryptococcus, and Trichoderma spp. were the most prevalent/abundant taxa. Advanced caries was associated with significantly higher fungal load and prevalence/abundance of Candida albicans. Cryptococcus neoformans and Candida sake were significantly over-abundant in early caries, while Malassezia globosa was significantly enriched in caries-free subjects. C. albicans correlated with Streptococcus mutans and Scardovia wiggsiae among other caries-associated bacteria, while M. globosa inversely correlated with caries-associated bacteria. In-vitro, M. globosa demonstrated inhibitory properties against S. mutans. Conclusions: the results substantiate the potential role of the oral mycobiome, primarily Candida species, in dental caries. Inter-kingdom correlations and inhibition of S. mutans by M. globosa are worth further investigation. |
| first_indexed | 2024-12-20T06:48:43Z |
| format | Article |
| id | doaj.art-c319f604bd30494ab9659625cc64c42c |
| institution | Directory Open Access Journal |
| issn | 2000-2297 |
| language | English |
| last_indexed | 2024-12-20T06:48:43Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj.art-c319f604bd30494ab9659625cc64c42c2022-12-21T19:49:38ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972020-01-0112110.1080/20002297.2020.17293051729305Supragingival mycobiome and inter-kingdom interactions in dental cariesDivyashri Baraniya0Tsute Chen1Anubhav Nahar2Fadhl Alakwaa3Jennifer Hill4Marisol Tellez5Amid Ismail6Sumant Puri7Nezar Noor Al-Hebshi8Temple UniversityForsyth InstituteTemple UniversityUniversity MichiganTemple UniversityTemple UniversityTemple UniversityTemple UniversityTemple UniversityBackground: Recent studies have reveled the presence of a complex fungal community (mycobiome) in the oral cavity. However, the role of oral mycobiome in dental caries and its interaction with caries-associated bacteria is not yet clear. Methods: Whole-mouth supragingival plaque samples from 30 children (6–10 years old) with no caries, early caries, or advanced caries were sequenced for internal transcribed spacer 2 (ITS-2). The mycobiome profiles were correlated with previously published bacteriome counterparts. Interaction among selected fungal and bacterial species was assessed by co-culture or spent media experiments. Results: Fungal load was extremely low. Candida, Malassezia, Cryptococcus, and Trichoderma spp. were the most prevalent/abundant taxa. Advanced caries was associated with significantly higher fungal load and prevalence/abundance of Candida albicans. Cryptococcus neoformans and Candida sake were significantly over-abundant in early caries, while Malassezia globosa was significantly enriched in caries-free subjects. C. albicans correlated with Streptococcus mutans and Scardovia wiggsiae among other caries-associated bacteria, while M. globosa inversely correlated with caries-associated bacteria. In-vitro, M. globosa demonstrated inhibitory properties against S. mutans. Conclusions: the results substantiate the potential role of the oral mycobiome, primarily Candida species, in dental caries. Inter-kingdom correlations and inhibition of S. mutans by M. globosa are worth further investigation.http://dx.doi.org/10.1080/20002297.2020.1729305bacteriadental cariesdental plaquehigh-throughput nucleotide sequencingmycobiome |
| spellingShingle | Divyashri Baraniya Tsute Chen Anubhav Nahar Fadhl Alakwaa Jennifer Hill Marisol Tellez Amid Ismail Sumant Puri Nezar Noor Al-Hebshi Supragingival mycobiome and inter-kingdom interactions in dental caries Journal of Oral Microbiology bacteria dental caries dental plaque high-throughput nucleotide sequencing mycobiome |
| title | Supragingival mycobiome and inter-kingdom interactions in dental caries |
| title_full | Supragingival mycobiome and inter-kingdom interactions in dental caries |
| title_fullStr | Supragingival mycobiome and inter-kingdom interactions in dental caries |
| title_full_unstemmed | Supragingival mycobiome and inter-kingdom interactions in dental caries |
| title_short | Supragingival mycobiome and inter-kingdom interactions in dental caries |
| title_sort | supragingival mycobiome and inter kingdom interactions in dental caries |
| topic | bacteria dental caries dental plaque high-throughput nucleotide sequencing mycobiome |
| url | http://dx.doi.org/10.1080/20002297.2020.1729305 |
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