Granulocyte-colony stimulating factor prevents the development of hepatic steatosis in rats
Background and aims. Previously, we reported that granulocyte-colony stimulating factor (G-CSF) improves hepatic steatosis in experimental animals. It may also have preventive effects on the development of hepatic steatosis. Therefore, we investigated the preventive effects of G-CSF by using a high-...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-03-01
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| Series: | Annals of Hepatology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1665268119307872 |
| _version_ | 1818602779055226880 |
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| author | Yi-Sun Song Hyun-Woo Joo In-Hwa Park Guang-Yin Shen Yonggu Lee Jeong Hun Shin Hyuck Kim Kyung-Soo Kim |
| author_facet | Yi-Sun Song Hyun-Woo Joo In-Hwa Park Guang-Yin Shen Yonggu Lee Jeong Hun Shin Hyuck Kim Kyung-Soo Kim |
| author_sort | Yi-Sun Song |
| collection | DOAJ |
| description | Background and aims. Previously, we reported that granulocyte-colony stimulating factor (G-CSF) improves hepatic steatosis in experimental animals. It may also have preventive effects on the development of hepatic steatosis. Therefore, we investigated the preventive effects of G-CSF by using a high-fat diet (HFD) rat model. Materials and methods. Twelve rats were fed HFD and 6 rats were fed control diet from 10 weeks of age. Once little steatosis was confirmed in the liver (after 10 weeks of feeding the HFD; at 20 weeks of age), HFD rats were randomly divided into two groups and treated with either G-CSF (100 Mg kg-1 day-1 for 5 consecutive days every other week; HFD/G-CSF rats) or saline (HFD/saline rats) for 10 weeks at 20 weeks of age. All rats were sacrificed at 30 weeks of age. Histology was examined by hematoxylin and eosin (H-E) and Oil Red O staining, and the expression levels of genes of associated with lipogenesis and β-oxidation enzymes were determined by qRT-PCR.Results. Histological examinations revealed that HFD/G-CSF rats had significantly lower lipid accumulation in their hepatocytes than did HFD/saline rats (p < 0.05). HFD/G-CSF rats also showed lower expression levels of genes associated with lipogenesis and higher expression levels of genes associated with β-oxidation than HFD/saline rats (p < 0.05). Conclusion. In conclusion, we found that G-CSF prevented development of hepatic steatosis in an HFD rat model. The preventive effect may be associated with the regulation of gene expression involved in hepatic lipogenesis and β-oxidation. |
| first_indexed | 2024-12-16T13:12:42Z |
| format | Article |
| id | doaj.art-c31a4cb5e3a24bb0926aea4f5b6925b1 |
| institution | Directory Open Access Journal |
| issn | 1665-2681 |
| language | English |
| last_indexed | 2024-12-16T13:12:42Z |
| publishDate | 2015-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Annals of Hepatology |
| spelling | doaj.art-c31a4cb5e3a24bb0926aea4f5b6925b12022-12-21T22:30:34ZengElsevierAnnals of Hepatology1665-26812015-03-01142243250Granulocyte-colony stimulating factor prevents the development of hepatic steatosis in ratsYi-Sun Song0Hyun-Woo Joo1In-Hwa Park2Guang-Yin Shen3Yonggu Lee4Jeong Hun Shin5Hyuck Kim6Kyung-Soo Kim7Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, South KoreaGraduate School of Biomedical Science and Engineering, Hanyang University, Seoul, South KoreaGraduate School of Biomedical Science and Engineering, Hanyang University, Seoul, South KoreaDepartment of Internal Medicine, Hanyang University College of Medicine, Seoul, South KoreaDepartment of Internal Medicine, Hanyang University College of Medicine, Seoul, South Korea; Department of Cardiology, Sung-Ae Hospital, Seoul, South KoreaDepartment of Internal Medicine, Hanyang University College of Medicine, Seoul, South KoreaDepartment of Thoracic and Cardiovascular Surgery, Hanyang University College of Medicine, Seoul, South KoreaGraduate School of Biomedical Science and Engineering, Hanyang University, Seoul, South Korea; Department of Internal Medicine, Hanyang University College of Medicine, Seoul, South Korea; Correspondence and reprint request:Background and aims. Previously, we reported that granulocyte-colony stimulating factor (G-CSF) improves hepatic steatosis in experimental animals. It may also have preventive effects on the development of hepatic steatosis. Therefore, we investigated the preventive effects of G-CSF by using a high-fat diet (HFD) rat model. Materials and methods. Twelve rats were fed HFD and 6 rats were fed control diet from 10 weeks of age. Once little steatosis was confirmed in the liver (after 10 weeks of feeding the HFD; at 20 weeks of age), HFD rats were randomly divided into two groups and treated with either G-CSF (100 Mg kg-1 day-1 for 5 consecutive days every other week; HFD/G-CSF rats) or saline (HFD/saline rats) for 10 weeks at 20 weeks of age. All rats were sacrificed at 30 weeks of age. Histology was examined by hematoxylin and eosin (H-E) and Oil Red O staining, and the expression levels of genes of associated with lipogenesis and β-oxidation enzymes were determined by qRT-PCR.Results. Histological examinations revealed that HFD/G-CSF rats had significantly lower lipid accumulation in their hepatocytes than did HFD/saline rats (p < 0.05). HFD/G-CSF rats also showed lower expression levels of genes associated with lipogenesis and higher expression levels of genes associated with β-oxidation than HFD/saline rats (p < 0.05). Conclusion. In conclusion, we found that G-CSF prevented development of hepatic steatosis in an HFD rat model. The preventive effect may be associated with the regulation of gene expression involved in hepatic lipogenesis and β-oxidation.http://www.sciencedirect.com/science/article/pii/S1665268119307872High-fat dietLipogenesisβ-oxidation |
| spellingShingle | Yi-Sun Song Hyun-Woo Joo In-Hwa Park Guang-Yin Shen Yonggu Lee Jeong Hun Shin Hyuck Kim Kyung-Soo Kim Granulocyte-colony stimulating factor prevents the development of hepatic steatosis in rats Annals of Hepatology High-fat diet Lipogenesis β-oxidation |
| title | Granulocyte-colony stimulating factor prevents the development of hepatic steatosis in rats |
| title_full | Granulocyte-colony stimulating factor prevents the development of hepatic steatosis in rats |
| title_fullStr | Granulocyte-colony stimulating factor prevents the development of hepatic steatosis in rats |
| title_full_unstemmed | Granulocyte-colony stimulating factor prevents the development of hepatic steatosis in rats |
| title_short | Granulocyte-colony stimulating factor prevents the development of hepatic steatosis in rats |
| title_sort | granulocyte colony stimulating factor prevents the development of hepatic steatosis in rats |
| topic | High-fat diet Lipogenesis β-oxidation |
| url | http://www.sciencedirect.com/science/article/pii/S1665268119307872 |
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