Synergistic antibacterial activity of compact silver/magnetite core-shell nanoparticles core shell against Gram-negative foodborne pathogens
The development of innovative antibacterial drugs against foodborne pathogens has led to an interest in novel materials such as nanomaterials. The unique features of nanomaterial qualify it for use as an antibacterial treatment. Noble metals and metal oxide nanoparticles, such as silver and magnetit...
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| Format: | Article |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.929491/full |
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| author | Eman M. Sharaf Amr Hassan Fawziah A. AL-Salmi Fauzeya M. Albalwe Hessa Meteq R. Albalawi Doaa B. Darwish Eman Fayad |
| author_facet | Eman M. Sharaf Amr Hassan Fawziah A. AL-Salmi Fauzeya M. Albalwe Hessa Meteq R. Albalawi Doaa B. Darwish Eman Fayad |
| author_sort | Eman M. Sharaf |
| collection | DOAJ |
| description | The development of innovative antibacterial drugs against foodborne pathogens has led to an interest in novel materials such as nanomaterials. The unique features of nanomaterial qualify it for use as an antibacterial treatment. Noble metals and metal oxide nanoparticles, such as silver and magnetite nanoparticles, have been shown to be effective antibacterial medications against a range of microorganisms. In this work, Ag@Fe3O4 -NPs were fabricated by using a wet chemical reduction and modified co-precipitation techniques. The antibacterial efficiency of the Ag/Fe3O4 core shell nanoparticles was investigated by applying various techniques, such as the Kirby–Bauer Disk Diffusion test, minimum inhibitory concentration (MIC) and bactericidal concentration (MBC), Colony Forming Unit (CFU), and kill time assay. The toxicity mechanism of Ag@Fe3O4 -NPs against Salmonella typhimurium and Escherichia coli was studied by apoptosis and reactive oxygen species (ROS) assays. The data revealed that a cubic core was surrounded by a silver shell, which indicated the regular morphology of silver magnetite core shell nanoparticles without any aggregation. Furthermore, Ag@Fe3O4 -NPs is more toxic against S. typhimurium and E. coli than Ag-NPs and Fe3O4 NPs. The MIC values for Ag/Fe3O4 NPs against S. typhimurium and E. coli were 3.1 and 5.4 μg/ml, respectively, whereas the MIC values for Ag-NPs and MNPs against S. typhimurium and E. coli were 4.1 and 8.2 μg/ml for Ag-NPs and 6.9 and 10.3 μg/ml for MNPs. The results showed the ability of Ag@Fe3O4 -NPs to induce apoptosis by generating ROS. Also, the ability of Ag@Fe3O4 -NPs to liberate free Ag+ and generate ROS via the Haber-Weiss cycle may be a plausible mechanism to explain the toxicity of Ag@Fe3O4 -NPs - NPs. |
| first_indexed | 2024-04-11T11:03:19Z |
| format | Article |
| id | doaj.art-c31a8b8aae344cdebcc4458f0f497cf6 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T11:03:19Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-c31a8b8aae344cdebcc4458f0f497cf62022-12-22T04:28:29ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-09-011310.3389/fmicb.2022.929491929491Synergistic antibacterial activity of compact silver/magnetite core-shell nanoparticles core shell against Gram-negative foodborne pathogensEman M. Sharaf0Amr Hassan1Fawziah A. AL-Salmi2Fauzeya M. Albalwe3Hessa Meteq R. Albalawi4Doaa B. Darwish5Eman Fayad6Department of Bacteriology, Immunology, and Mycology, Animal Health Research Institute (AHRI), Shebin El Kom, EgyptDepartment of Bioinformatics, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat, EgyptDepartment of Biology, Faculty of Sciences, Taif University, Taif, Saudi ArabiaDepartment of Biology, Faculty of Science, Tabuk University, Tabuk, Saudi ArabiaDepartment of Pharmacy, Prince Sultan Armed Forces Hospital, Medina, Saudi ArabiaDepartment of Botany, Faculty of Science, Mansoura University, Mansoura, EgyptDepartment of Biotechnology, Faculty of Sciences, Taif University, Taif, Saudi ArabiaThe development of innovative antibacterial drugs against foodborne pathogens has led to an interest in novel materials such as nanomaterials. The unique features of nanomaterial qualify it for use as an antibacterial treatment. Noble metals and metal oxide nanoparticles, such as silver and magnetite nanoparticles, have been shown to be effective antibacterial medications against a range of microorganisms. In this work, Ag@Fe3O4 -NPs were fabricated by using a wet chemical reduction and modified co-precipitation techniques. The antibacterial efficiency of the Ag/Fe3O4 core shell nanoparticles was investigated by applying various techniques, such as the Kirby–Bauer Disk Diffusion test, minimum inhibitory concentration (MIC) and bactericidal concentration (MBC), Colony Forming Unit (CFU), and kill time assay. The toxicity mechanism of Ag@Fe3O4 -NPs against Salmonella typhimurium and Escherichia coli was studied by apoptosis and reactive oxygen species (ROS) assays. The data revealed that a cubic core was surrounded by a silver shell, which indicated the regular morphology of silver magnetite core shell nanoparticles without any aggregation. Furthermore, Ag@Fe3O4 -NPs is more toxic against S. typhimurium and E. coli than Ag-NPs and Fe3O4 NPs. The MIC values for Ag/Fe3O4 NPs against S. typhimurium and E. coli were 3.1 and 5.4 μg/ml, respectively, whereas the MIC values for Ag-NPs and MNPs against S. typhimurium and E. coli were 4.1 and 8.2 μg/ml for Ag-NPs and 6.9 and 10.3 μg/ml for MNPs. The results showed the ability of Ag@Fe3O4 -NPs to induce apoptosis by generating ROS. Also, the ability of Ag@Fe3O4 -NPs to liberate free Ag+ and generate ROS via the Haber-Weiss cycle may be a plausible mechanism to explain the toxicity of Ag@Fe3O4 -NPs - NPs.https://www.frontiersin.org/articles/10.3389/fmicb.2022.929491/fullfoodborne pathogenssilver/magnetite core shell nanoparticleGram negativereactive oxygen speciesSalmonella typhimuriumEscherichia coli |
| spellingShingle | Eman M. Sharaf Amr Hassan Fawziah A. AL-Salmi Fauzeya M. Albalwe Hessa Meteq R. Albalawi Doaa B. Darwish Eman Fayad Synergistic antibacterial activity of compact silver/magnetite core-shell nanoparticles core shell against Gram-negative foodborne pathogens Frontiers in Microbiology foodborne pathogens silver/magnetite core shell nanoparticle Gram negative reactive oxygen species Salmonella typhimurium Escherichia coli |
| title | Synergistic antibacterial activity of compact silver/magnetite core-shell nanoparticles core shell against Gram-negative foodborne pathogens |
| title_full | Synergistic antibacterial activity of compact silver/magnetite core-shell nanoparticles core shell against Gram-negative foodborne pathogens |
| title_fullStr | Synergistic antibacterial activity of compact silver/magnetite core-shell nanoparticles core shell against Gram-negative foodborne pathogens |
| title_full_unstemmed | Synergistic antibacterial activity of compact silver/magnetite core-shell nanoparticles core shell against Gram-negative foodborne pathogens |
| title_short | Synergistic antibacterial activity of compact silver/magnetite core-shell nanoparticles core shell against Gram-negative foodborne pathogens |
| title_sort | synergistic antibacterial activity of compact silver magnetite core shell nanoparticles core shell against gram negative foodborne pathogens |
| topic | foodborne pathogens silver/magnetite core shell nanoparticle Gram negative reactive oxygen species Salmonella typhimurium Escherichia coli |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.929491/full |
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