Novel Crown Cement Containing Antibacterial Monomer and Calcium Phosphate Nanoparticles
Oral biofilm accumulation at the tooth–restoration interface often leads to recurrent dental caries and restoration failure. The objectives of this study were to: (1) develop a novel bioactive crown cement containing dimethylaminohexadecyl methacrylate (DMAHDM) and nano-sized amorphous calcium phosp...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/10/10/2001 |
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| author | Rashed AlSahafi Abdulrahman A. Balhaddad Heba Mitwalli Maria Salem Ibrahim Mary Anne S. Melo Thomas W. Oates Hockin H.K. Xu Michael D. Weir |
| author_facet | Rashed AlSahafi Abdulrahman A. Balhaddad Heba Mitwalli Maria Salem Ibrahim Mary Anne S. Melo Thomas W. Oates Hockin H.K. Xu Michael D. Weir |
| author_sort | Rashed AlSahafi |
| collection | DOAJ |
| description | Oral biofilm accumulation at the tooth–restoration interface often leads to recurrent dental caries and restoration failure. The objectives of this study were to: (1) develop a novel bioactive crown cement containing dimethylaminohexadecyl methacrylate (DMAHDM) and nano-sized amorphous calcium phosphate (NACP), and (2) investigate the mechanical properties, anti-biofilm activity, and calcium (Ca<sup>2+</sup>) and phosphate (PO<sub>4</sub><sup>3−</sup>) ion release of the crown cement for the first time. The cement matrix consisted of pyromellitic glycerol dimethacrylate and ethoxylated bisphenol-A dimethacrylate monomers and was denoted PEHB resin matrix. The following cements were tested: (1) RelyX luting cement (commercial control); (2) 55% PEHB + 45% glass fillers (experimental control); (3) 55% PEHB + 20% glass + 25% NACP + 0% DMAHDM; (4) 52% PEHB + 20% glass + 25% NACP + 3% DMAHDM; (5) 51% PEHB + 20% glass + 25% NACP + 4% DMAHDM; (6) 50% PEHB + 20% glass + 25% NACP + 5% DMAHDM. Mechanical properties and ion release were measured. <i>Streptococcus</i><i>mutans</i> (<i>S. mutans</i>) biofilms were grown on cements, and colony-forming units (CFUs) and other biofilm properties were measured. The novel bioactive cement demonstrated strong antibacterial properties and high levels of Ca<sup>2+</sup> and PO<sub>4</sub><sup>3−</sup> ion release to remineralize tooth lesions. Adding NACP and DMAHDM into the cement did not adversely affect the mechanical properties and dentin bonding strength. In conclusion, the novel NACP + DMAHDM crown cement has excellent potential for restoration cementation to inhibit caries by suppressing oral biofilm growth and increasing remineralization via Ca<sup>2+</sup> and PO<sub>4</sub><sup>3−</sup> ions. The NACP + DMAHDM composition may have wide applicability to other biomaterials to promote hard-tissue formation and combat bacterial infection. |
| first_indexed | 2024-03-10T15:43:46Z |
| format | Article |
| id | doaj.art-c31c40d33c474bf59bb466a8d0815d65 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T15:43:46Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-c31c40d33c474bf59bb466a8d0815d652023-11-20T16:36:53ZengMDPI AGNanomaterials2079-49912020-10-011010200110.3390/nano10102001Novel Crown Cement Containing Antibacterial Monomer and Calcium Phosphate NanoparticlesRashed AlSahafi0Abdulrahman A. Balhaddad1Heba Mitwalli2Maria Salem Ibrahim3Mary Anne S. Melo4Thomas W. Oates5Hockin H.K. Xu6Michael D. Weir7Program in Dental Biomedical Sciences, School of Dentistry, University of Maryland, Baltimore, MD 21201, USAProgram in Dental Biomedical Sciences, School of Dentistry, University of Maryland, Baltimore, MD 21201, USAProgram in Dental Biomedical Sciences, School of Dentistry, University of Maryland, Baltimore, MD 21201, USADepartment of Preventive Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi ArabiaDepartment of General Dentistry, Division of Operative Dentistry, School of Dentistry, University of Maryland, Baltimore, MD 21201, USADepartment of Advanced Oral Sciences and Therapeutics, Biomaterials & Tissue Engineering Division, School of Dentistry, University of Maryland, Baltimore, MD 21201, USADepartment of Advanced Oral Sciences and Therapeutics, Biomaterials & Tissue Engineering Division, School of Dentistry, University of Maryland, Baltimore, MD 21201, USADepartment of Advanced Oral Sciences and Therapeutics, Biomaterials & Tissue Engineering Division, School of Dentistry, University of Maryland, Baltimore, MD 21201, USAOral biofilm accumulation at the tooth–restoration interface often leads to recurrent dental caries and restoration failure. The objectives of this study were to: (1) develop a novel bioactive crown cement containing dimethylaminohexadecyl methacrylate (DMAHDM) and nano-sized amorphous calcium phosphate (NACP), and (2) investigate the mechanical properties, anti-biofilm activity, and calcium (Ca<sup>2+</sup>) and phosphate (PO<sub>4</sub><sup>3−</sup>) ion release of the crown cement for the first time. The cement matrix consisted of pyromellitic glycerol dimethacrylate and ethoxylated bisphenol-A dimethacrylate monomers and was denoted PEHB resin matrix. The following cements were tested: (1) RelyX luting cement (commercial control); (2) 55% PEHB + 45% glass fillers (experimental control); (3) 55% PEHB + 20% glass + 25% NACP + 0% DMAHDM; (4) 52% PEHB + 20% glass + 25% NACP + 3% DMAHDM; (5) 51% PEHB + 20% glass + 25% NACP + 4% DMAHDM; (6) 50% PEHB + 20% glass + 25% NACP + 5% DMAHDM. Mechanical properties and ion release were measured. <i>Streptococcus</i><i>mutans</i> (<i>S. mutans</i>) biofilms were grown on cements, and colony-forming units (CFUs) and other biofilm properties were measured. The novel bioactive cement demonstrated strong antibacterial properties and high levels of Ca<sup>2+</sup> and PO<sub>4</sub><sup>3−</sup> ion release to remineralize tooth lesions. Adding NACP and DMAHDM into the cement did not adversely affect the mechanical properties and dentin bonding strength. In conclusion, the novel NACP + DMAHDM crown cement has excellent potential for restoration cementation to inhibit caries by suppressing oral biofilm growth and increasing remineralization via Ca<sup>2+</sup> and PO<sub>4</sub><sup>3−</sup> ions. The NACP + DMAHDM composition may have wide applicability to other biomaterials to promote hard-tissue formation and combat bacterial infection.https://www.mdpi.com/2079-4991/10/10/2001calcium phosphate nanoparticlesantibacterial monomerdental cariescrown cementoral biofilms<i>Streptococcus mutans</i> |
| spellingShingle | Rashed AlSahafi Abdulrahman A. Balhaddad Heba Mitwalli Maria Salem Ibrahim Mary Anne S. Melo Thomas W. Oates Hockin H.K. Xu Michael D. Weir Novel Crown Cement Containing Antibacterial Monomer and Calcium Phosphate Nanoparticles Nanomaterials calcium phosphate nanoparticles antibacterial monomer dental caries crown cement oral biofilms <i>Streptococcus mutans</i> |
| title | Novel Crown Cement Containing Antibacterial Monomer and Calcium Phosphate Nanoparticles |
| title_full | Novel Crown Cement Containing Antibacterial Monomer and Calcium Phosphate Nanoparticles |
| title_fullStr | Novel Crown Cement Containing Antibacterial Monomer and Calcium Phosphate Nanoparticles |
| title_full_unstemmed | Novel Crown Cement Containing Antibacterial Monomer and Calcium Phosphate Nanoparticles |
| title_short | Novel Crown Cement Containing Antibacterial Monomer and Calcium Phosphate Nanoparticles |
| title_sort | novel crown cement containing antibacterial monomer and calcium phosphate nanoparticles |
| topic | calcium phosphate nanoparticles antibacterial monomer dental caries crown cement oral biofilms <i>Streptococcus mutans</i> |
| url | https://www.mdpi.com/2079-4991/10/10/2001 |
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