Preliminary Results of the in Vivo and in Vitro Characterization of a Tentacle Venom Fraction from the Jellyfish Aurelia aurita
The neurotoxic effects produced by a tentacle venom extract and a fraction were analyzed and correlated by in vivo and in vitro approaches. The tentacle venom extract exhibited a wide range of protein components (from 24 to >225 kDa) and produced tetanic reactions, flaccid paralysis, and deat...
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| Format: | Article |
| Language: | English |
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MDPI AG
2013-12-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/5/12/2420 |
| _version_ | 1817990792025735168 |
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| author | Dalia Ponce Estuardo López-Vera Manuel B. Aguilar Judith Sánchez-Rodríguez |
| author_facet | Dalia Ponce Estuardo López-Vera Manuel B. Aguilar Judith Sánchez-Rodríguez |
| author_sort | Dalia Ponce |
| collection | DOAJ |
| description | The neurotoxic effects produced by a tentacle venom extract and a fraction were analyzed and correlated by in vivo and in vitro approaches. The tentacle venom extract exhibited a wide range of protein components (from 24 to >225 kDa) and produced tetanic reactions, flaccid paralysis, and death when injected into crabs. Two chromatography fractions also produced uncontrolled appendix movements and leg stretching. Further electrophysiological characterization demonstrated that one of these fractions potently inhibited ACh-elicited currents mediated by both vertebrate fetal and adult muscle nicotinic acetylcholine receptors (nAChR) subtypes. Receptor inhibition was concentration-dependent and completely reversible. The calculated IC50 values were 1.77 μg/μL for fetal and 2.28 μg/μL for adult muscle nAChRs. The bioactive fraction was composed of a major protein component at ~90 kDa and lacked phospholipase A activity. This work represents the first insight into the interaction of jellyfish venom components and muscle nicotinic receptors. |
| first_indexed | 2024-04-14T01:04:46Z |
| format | Article |
| id | doaj.art-c31d4daaef1b42b7833ca567c7b09399 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-14T01:04:46Z |
| publishDate | 2013-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-c31d4daaef1b42b7833ca567c7b093992022-12-22T02:21:18ZengMDPI AGToxins2072-66512013-12-015122420243310.3390/toxins5122420toxins5122420Preliminary Results of the in Vivo and in Vitro Characterization of a Tentacle Venom Fraction from the Jellyfish Aurelia auritaDalia Ponce0Estuardo López-Vera1Manuel B. Aguilar2Judith Sánchez-Rodríguez3Instituto de Ciencias del Mar y Limnología, Unidad Académica Puerto Morelos, Universidad Nacional Autónoma de México,77500 Cancún, Quintana Roo, MexicoInstituto de Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Ciudad Universitaria, 04510 Coyoacán, Distrito Federal, MexicoLaboratorio de Neurofarmacología Marina, Departamento de Neurobiología Celular y Molecular, Instituto de Neurobiología, Universidad Nacional Autónoma de México, Campus Juriquilla, Juriquilla 76230, Querétaro, MexicoInstituto de Ciencias del Mar y Limnología, Unidad Académica Puerto Morelos, Universidad Nacional Autónoma de México,77500 Cancún, Quintana Roo, MexicoThe neurotoxic effects produced by a tentacle venom extract and a fraction were analyzed and correlated by in vivo and in vitro approaches. The tentacle venom extract exhibited a wide range of protein components (from 24 to >225 kDa) and produced tetanic reactions, flaccid paralysis, and death when injected into crabs. Two chromatography fractions also produced uncontrolled appendix movements and leg stretching. Further electrophysiological characterization demonstrated that one of these fractions potently inhibited ACh-elicited currents mediated by both vertebrate fetal and adult muscle nicotinic acetylcholine receptors (nAChR) subtypes. Receptor inhibition was concentration-dependent and completely reversible. The calculated IC50 values were 1.77 μg/μL for fetal and 2.28 μg/μL for adult muscle nAChRs. The bioactive fraction was composed of a major protein component at ~90 kDa and lacked phospholipase A activity. This work represents the first insight into the interaction of jellyfish venom components and muscle nicotinic receptors.http://www.mdpi.com/2072-6651/5/12/2420Aurelia auritajellyfishneurotoxinsnicotinic acetylcholine receptorsScyphozoa |
| spellingShingle | Dalia Ponce Estuardo López-Vera Manuel B. Aguilar Judith Sánchez-Rodríguez Preliminary Results of the in Vivo and in Vitro Characterization of a Tentacle Venom Fraction from the Jellyfish Aurelia aurita Toxins Aurelia aurita jellyfish neurotoxins nicotinic acetylcholine receptors Scyphozoa |
| title | Preliminary Results of the in Vivo and in Vitro Characterization of a Tentacle Venom Fraction from the Jellyfish Aurelia aurita |
| title_full | Preliminary Results of the in Vivo and in Vitro Characterization of a Tentacle Venom Fraction from the Jellyfish Aurelia aurita |
| title_fullStr | Preliminary Results of the in Vivo and in Vitro Characterization of a Tentacle Venom Fraction from the Jellyfish Aurelia aurita |
| title_full_unstemmed | Preliminary Results of the in Vivo and in Vitro Characterization of a Tentacle Venom Fraction from the Jellyfish Aurelia aurita |
| title_short | Preliminary Results of the in Vivo and in Vitro Characterization of a Tentacle Venom Fraction from the Jellyfish Aurelia aurita |
| title_sort | preliminary results of the in vivo and in vitro characterization of a tentacle venom fraction from the jellyfish aurelia aurita |
| topic | Aurelia aurita jellyfish neurotoxins nicotinic acetylcholine receptors Scyphozoa |
| url | http://www.mdpi.com/2072-6651/5/12/2420 |
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