Molecular characterization and evolutionary history analysis of a novel Kudzu mosaic virus (KMV) infecting the Kudzu (Pueraria montana)
Objectives: Plant viruses are a significant contributor to the economic losses experienced in numerous plant species. Begomoviruses exhibit a wide range of host species, encompassing both cultivated and non-cultivated plants. The kudzu plant, scientifically known as Pueraria montana, is indigenous t...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364723004524 |
| _version_ | 1797454379966529536 |
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| author | Muhammad Waheed Riaz Muhammad Arif Yasir Mehmood Mehmet Ertugrul Guldur Shikhah K. Alnemar Mona S. Alwahibi Aicun Zhou |
| author_facet | Muhammad Waheed Riaz Muhammad Arif Yasir Mehmood Mehmet Ertugrul Guldur Shikhah K. Alnemar Mona S. Alwahibi Aicun Zhou |
| author_sort | Muhammad Waheed Riaz |
| collection | DOAJ |
| description | Objectives: Plant viruses are a significant contributor to the economic losses experienced in numerous plant species. Begomoviruses exhibit a wide range of host species, encompassing both cultivated and non-cultivated plants. The kudzu plant, scientifically known as Pueraria montana, is indigenous to the Southeast Asian region. Moreover, it exhibits varied attributes with regards to its utilization in the fields of medicine, food, cosmetics, and the herbal industry. Methods: During a routine survey, some P. montana plants having typical symptoms of yellowing, mosaic, and mottle were observed in areas including Fort-Minro (FM) and Anari of district Dera Ghazi Khan and Maari (MA) of district Rajanpur. The genomic DNA was extracted from the collected samples. The polymerase chain reaction (PCR) and rolling circular amplification (RCA) was carried out to isolate the DNA and DNA- β from the collected plant specimens. An evolutionary research was conducted to assess the lineage of this virus inside a novel geographical region. Results: A 500-bp amplicon size was obtained from the samples of the FM and MA, while the approximately 2.7-kb amplicon size of its full genome was obtained from the samples of both locations. The results of the sequence analysis demonstrated conclusively that the plants in question are infected with Kudzu mosaic virus (KMV). The evolutionary history analysis has confirmed the virus's lineage in a new region, and the nucleotide frequencies of obtained sequences were variable. Conclusion: The presence of a novel bipartite begomovirus in the tropical region has been substantiated using a phylogenetic and evolutionary investigation. The observation of a white fly population on infected plants has also suggested that the transmission of this virus through the migration of this insect vector can be a viable mechanism. |
| first_indexed | 2024-03-09T15:36:23Z |
| format | Article |
| id | doaj.art-c32025a8961d4e049a222c9cdd569f82 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-03-09T15:36:23Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-c32025a8961d4e049a222c9cdd569f822023-11-26T05:11:56ZengElsevierJournal of King Saud University: Science1018-36472023-12-013511102990Molecular characterization and evolutionary history analysis of a novel Kudzu mosaic virus (KMV) infecting the Kudzu (Pueraria montana)Muhammad Waheed Riaz0Muhammad Arif1Yasir Mehmood2Mehmet Ertugrul Guldur3Shikhah K. Alnemar4Mona S. Alwahibi5Aicun Zhou6State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, ChinaDepartment of Plant Protection, Faculty of Agriculture, Sakarya University of Applied Sciences, Arifiye, 54580, Sakarya, Türkiye; Corresponding authors.Department of Plant Pathology, Faculty of Agriculture Sciences and Technology, Bahauddin Zakariya University, Multan, 60700, Punjab, PakistanDepartment of Plant Protection, Faculty of Agriculture, Harran University, Sanliurfa, TürkiyeDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi ArabiaState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, China; Zhaoming Materia Medica Research Institute of Pan'an County, Jinhua, Zhejiang 321000, PR China; Corresponding authors.Objectives: Plant viruses are a significant contributor to the economic losses experienced in numerous plant species. Begomoviruses exhibit a wide range of host species, encompassing both cultivated and non-cultivated plants. The kudzu plant, scientifically known as Pueraria montana, is indigenous to the Southeast Asian region. Moreover, it exhibits varied attributes with regards to its utilization in the fields of medicine, food, cosmetics, and the herbal industry. Methods: During a routine survey, some P. montana plants having typical symptoms of yellowing, mosaic, and mottle were observed in areas including Fort-Minro (FM) and Anari of district Dera Ghazi Khan and Maari (MA) of district Rajanpur. The genomic DNA was extracted from the collected samples. The polymerase chain reaction (PCR) and rolling circular amplification (RCA) was carried out to isolate the DNA and DNA- β from the collected plant specimens. An evolutionary research was conducted to assess the lineage of this virus inside a novel geographical region. Results: A 500-bp amplicon size was obtained from the samples of the FM and MA, while the approximately 2.7-kb amplicon size of its full genome was obtained from the samples of both locations. The results of the sequence analysis demonstrated conclusively that the plants in question are infected with Kudzu mosaic virus (KMV). The evolutionary history analysis has confirmed the virus's lineage in a new region, and the nucleotide frequencies of obtained sequences were variable. Conclusion: The presence of a novel bipartite begomovirus in the tropical region has been substantiated using a phylogenetic and evolutionary investigation. The observation of a white fly population on infected plants has also suggested that the transmission of this virus through the migration of this insect vector can be a viable mechanism.http://www.sciencedirect.com/science/article/pii/S1018364723004524KudzuBegomovirusesPhylogenetic analysisNucleotide comparisonMEGA11 |
| spellingShingle | Muhammad Waheed Riaz Muhammad Arif Yasir Mehmood Mehmet Ertugrul Guldur Shikhah K. Alnemar Mona S. Alwahibi Aicun Zhou Molecular characterization and evolutionary history analysis of a novel Kudzu mosaic virus (KMV) infecting the Kudzu (Pueraria montana) Journal of King Saud University: Science Kudzu Begomoviruses Phylogenetic analysis Nucleotide comparison MEGA11 |
| title | Molecular characterization and evolutionary history analysis of a novel Kudzu mosaic virus (KMV) infecting the Kudzu (Pueraria montana) |
| title_full | Molecular characterization and evolutionary history analysis of a novel Kudzu mosaic virus (KMV) infecting the Kudzu (Pueraria montana) |
| title_fullStr | Molecular characterization and evolutionary history analysis of a novel Kudzu mosaic virus (KMV) infecting the Kudzu (Pueraria montana) |
| title_full_unstemmed | Molecular characterization and evolutionary history analysis of a novel Kudzu mosaic virus (KMV) infecting the Kudzu (Pueraria montana) |
| title_short | Molecular characterization and evolutionary history analysis of a novel Kudzu mosaic virus (KMV) infecting the Kudzu (Pueraria montana) |
| title_sort | molecular characterization and evolutionary history analysis of a novel kudzu mosaic virus kmv infecting the kudzu pueraria montana |
| topic | Kudzu Begomoviruses Phylogenetic analysis Nucleotide comparison MEGA11 |
| url | http://www.sciencedirect.com/science/article/pii/S1018364723004524 |
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