Analysis of Endophyte Diversity of <i>Rheum palmatum</i> from Different Production Areas in Gansu Province of China and the Association with Secondary Metabolite
Investigations of the differences in the metabolites of medicinal plants have typically focused on the effects of external environmental factors. However, little is known about the relationship between endophytes diversity and host metabolites. We used high-throughput sequencing methods to compare t...
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| Format: | Article |
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MDPI AG
2021-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/5/978 |
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| author | Dawei Chen Lingyun Jia Qinzheng Hou Xiang Zhao Kun Sun |
| author_facet | Dawei Chen Lingyun Jia Qinzheng Hou Xiang Zhao Kun Sun |
| author_sort | Dawei Chen |
| collection | DOAJ |
| description | Investigations of the differences in the metabolites of medicinal plants have typically focused on the effects of external environmental factors. However, little is known about the relationship between endophytes diversity and host metabolites. We used high-throughput sequencing methods to compare the endophyte diversity of <i>Rheum palmatum</i> from eight different production areas in Gansu Province of China and to analyze the association between those areas and five secondary metabolites (aloe-emodin, rhein, emodin, chrysophanol, and physcion). The results show that the diversity and OTUs (Operational taxonomic units) abundance of endophytic fungi and bacteria of <i>R. palmatum</i> differed according to production area. Spearman analysis showed that the five secondary metabolites of <i>R. palmatum</i> were positively correlated with the diversity and abundance of endophytic fungi. Comparing both space and environmental differences to determine influences on community structure, VPA analysis revealed that geographic factors explained more difference in community composition of fungal and bacterial endophytes than climate factors. PICRUSt and FUNGuild predictive analysis indicated that metabolites were the primary components of endophytic bacteria in all samples, while the function of endophytic fungi was composed of dominant trophic modes (saprotroph and pathotroph), and relative abundances were different. Our results help elucidate the correlation of plant–microbe interactions and offer pivotal information to reveal the role of endophytes in the production of <i>R. palmatum</i> and its important secondary metabolite. |
| first_indexed | 2024-03-10T11:47:32Z |
| format | Article |
| id | doaj.art-c3220e81b15a4403b6aa8fe482052447 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T11:47:32Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-c3220e81b15a4403b6aa8fe4820524472023-11-21T18:01:04ZengMDPI AGMicroorganisms2076-26072021-04-019597810.3390/microorganisms9050978Analysis of Endophyte Diversity of <i>Rheum palmatum</i> from Different Production Areas in Gansu Province of China and the Association with Secondary MetaboliteDawei Chen0Lingyun Jia1Qinzheng Hou2Xiang Zhao3Kun Sun4College of Life Sciences, Northwest Normal University, Lanzhou 730070, ChinaCollege of Life Sciences, Northwest Normal University, Lanzhou 730070, ChinaCollege of Life Sciences, Northwest Normal University, Lanzhou 730070, ChinaCollege of Life Sciences, Northwest Normal University, Lanzhou 730070, ChinaCollege of Life Sciences, Northwest Normal University, Lanzhou 730070, ChinaInvestigations of the differences in the metabolites of medicinal plants have typically focused on the effects of external environmental factors. However, little is known about the relationship between endophytes diversity and host metabolites. We used high-throughput sequencing methods to compare the endophyte diversity of <i>Rheum palmatum</i> from eight different production areas in Gansu Province of China and to analyze the association between those areas and five secondary metabolites (aloe-emodin, rhein, emodin, chrysophanol, and physcion). The results show that the diversity and OTUs (Operational taxonomic units) abundance of endophytic fungi and bacteria of <i>R. palmatum</i> differed according to production area. Spearman analysis showed that the five secondary metabolites of <i>R. palmatum</i> were positively correlated with the diversity and abundance of endophytic fungi. Comparing both space and environmental differences to determine influences on community structure, VPA analysis revealed that geographic factors explained more difference in community composition of fungal and bacterial endophytes than climate factors. PICRUSt and FUNGuild predictive analysis indicated that metabolites were the primary components of endophytic bacteria in all samples, while the function of endophytic fungi was composed of dominant trophic modes (saprotroph and pathotroph), and relative abundances were different. Our results help elucidate the correlation of plant–microbe interactions and offer pivotal information to reveal the role of endophytes in the production of <i>R. palmatum</i> and its important secondary metabolite.https://www.mdpi.com/2076-2607/9/5/978<i>R. palmatum</i>endophytesdiversitycorrelation analysisecological function |
| spellingShingle | Dawei Chen Lingyun Jia Qinzheng Hou Xiang Zhao Kun Sun Analysis of Endophyte Diversity of <i>Rheum palmatum</i> from Different Production Areas in Gansu Province of China and the Association with Secondary Metabolite Microorganisms <i>R. palmatum</i> endophytes diversity correlation analysis ecological function |
| title | Analysis of Endophyte Diversity of <i>Rheum palmatum</i> from Different Production Areas in Gansu Province of China and the Association with Secondary Metabolite |
| title_full | Analysis of Endophyte Diversity of <i>Rheum palmatum</i> from Different Production Areas in Gansu Province of China and the Association with Secondary Metabolite |
| title_fullStr | Analysis of Endophyte Diversity of <i>Rheum palmatum</i> from Different Production Areas in Gansu Province of China and the Association with Secondary Metabolite |
| title_full_unstemmed | Analysis of Endophyte Diversity of <i>Rheum palmatum</i> from Different Production Areas in Gansu Province of China and the Association with Secondary Metabolite |
| title_short | Analysis of Endophyte Diversity of <i>Rheum palmatum</i> from Different Production Areas in Gansu Province of China and the Association with Secondary Metabolite |
| title_sort | analysis of endophyte diversity of i rheum palmatum i from different production areas in gansu province of china and the association with secondary metabolite |
| topic | <i>R. palmatum</i> endophytes diversity correlation analysis ecological function |
| url | https://www.mdpi.com/2076-2607/9/5/978 |
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