Exopolysaccharide Production and Prevention of Syneresis in Starch Using Encapsulated Probiotic Lactobacillus plantarum
Encapsulation of probiotic bacteria with a matrix can increase their survival rate by protecting them from adverse conditions and at the same time without affecting the production of metabolites. An effort has been made to encapsulate the probiotic Lactobacillus plantarum using calcium alginate. Box...
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| Format: | Article |
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University of Zagreb Faculty of Food Technology and Biotechnology
2010-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | http://hrcak.srce.hr/file/92473 |
| _version_ | 1797430558172643328 |
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| author | Bindhumol Ismail Kesavan Madhavan Nampoothiri |
| author_facet | Bindhumol Ismail Kesavan Madhavan Nampoothiri |
| author_sort | Bindhumol Ismail |
| collection | DOAJ |
| description | Encapsulation of probiotic bacteria with a matrix can increase their survival rate by protecting them from adverse conditions and at the same time without affecting the production of metabolites. An effort has been made to encapsulate the probiotic Lactobacillus plantarum using calcium alginate. Box-Behnken model of response surface methodology (RSM) was employed in the optimization of major encapsulation conditions such as concentration of sodium alginate, calcium chloride and curing time. The second-order quadratic model with the optimum conditions (sodium alginate 2 % (by mass per volume), calcium chloride 0.5 M and curing time 3 h) resulted in a maximum titre of (0.9±0.1) g/L of exopolysaccharides (EPS) at 72 h. The nearness of the coefficient of determination (R²=0.97) to 1 ensures the satisfactory adjustment of the quadratic model to the experimental data. The efficiency of EPS production by encapsulated cells was compared with free cells. The efficacy of secreted EPS in the prevention of syneresis in starch was investigated. |
| first_indexed | 2024-03-09T09:30:22Z |
| format | Article |
| id | doaj.art-c322d34d6e9445ecb743c7eed7b76d5b |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-03-09T09:30:22Z |
| publishDate | 2010-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
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| series | Food Technology and Biotechnology |
| spelling | doaj.art-c322d34d6e9445ecb743c7eed7b76d5b2023-12-02T04:22:18ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062010-01-01484484489Exopolysaccharide Production and Prevention of Syneresis in Starch Using Encapsulated Probiotic Lactobacillus plantarumBindhumol Ismail0Kesavan Madhavan Nampoothiri1National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, Trivandrum 695 019, Kerala, IndiaNational Institute for Interdisciplinary Science and Technology (NIIST), CSIR, Trivandrum 695 019, Kerala, IndiaEncapsulation of probiotic bacteria with a matrix can increase their survival rate by protecting them from adverse conditions and at the same time without affecting the production of metabolites. An effort has been made to encapsulate the probiotic Lactobacillus plantarum using calcium alginate. Box-Behnken model of response surface methodology (RSM) was employed in the optimization of major encapsulation conditions such as concentration of sodium alginate, calcium chloride and curing time. The second-order quadratic model with the optimum conditions (sodium alginate 2 % (by mass per volume), calcium chloride 0.5 M and curing time 3 h) resulted in a maximum titre of (0.9±0.1) g/L of exopolysaccharides (EPS) at 72 h. The nearness of the coefficient of determination (R²=0.97) to 1 ensures the satisfactory adjustment of the quadratic model to the experimental data. The efficiency of EPS production by encapsulated cells was compared with free cells. The efficacy of secreted EPS in the prevention of syneresis in starch was investigated.http://hrcak.srce.hr/file/92473encapsulationexopolysaccharidelactic acid bacteriaprobioticsresponse surface methodology (RSM)syneresis |
| spellingShingle | Bindhumol Ismail Kesavan Madhavan Nampoothiri Exopolysaccharide Production and Prevention of Syneresis in Starch Using Encapsulated Probiotic Lactobacillus plantarum Food Technology and Biotechnology encapsulation exopolysaccharide lactic acid bacteria probiotics response surface methodology (RSM) syneresis |
| title | Exopolysaccharide Production and Prevention of Syneresis in Starch Using Encapsulated Probiotic Lactobacillus plantarum |
| title_full | Exopolysaccharide Production and Prevention of Syneresis in Starch Using Encapsulated Probiotic Lactobacillus plantarum |
| title_fullStr | Exopolysaccharide Production and Prevention of Syneresis in Starch Using Encapsulated Probiotic Lactobacillus plantarum |
| title_full_unstemmed | Exopolysaccharide Production and Prevention of Syneresis in Starch Using Encapsulated Probiotic Lactobacillus plantarum |
| title_short | Exopolysaccharide Production and Prevention of Syneresis in Starch Using Encapsulated Probiotic Lactobacillus plantarum |
| title_sort | exopolysaccharide production and prevention of syneresis in starch using encapsulated probiotic lactobacillus plantarum |
| topic | encapsulation exopolysaccharide lactic acid bacteria probiotics response surface methodology (RSM) syneresis |
| url | http://hrcak.srce.hr/file/92473 |
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