Significance of miR-196b in tumor-related epilepsy of patients with gliomas.
OBJECTIVES: Seizure is a common presenting symptom of primary brain tumors. There are no published studies regarding the roles of MicroRNA (miRNA) in tumor-related epilepsy. The authors set out to correlate miR-196b expression in low-grade glioma patients with pre-operative seizures and post-operati...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3457999?pdf=render |
| _version_ | 1828932452070981632 |
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| author | Gan You Wei Yan Wei Zhang Yongzhi Wang Zhaoshi Bao Shouwei Li Shaowu Li Guilin Li Yijun Song Chunsheng Kang Tao Jiang |
| author_facet | Gan You Wei Yan Wei Zhang Yongzhi Wang Zhaoshi Bao Shouwei Li Shaowu Li Guilin Li Yijun Song Chunsheng Kang Tao Jiang |
| author_sort | Gan You |
| collection | DOAJ |
| description | OBJECTIVES: Seizure is a common presenting symptom of primary brain tumors. There are no published studies regarding the roles of MicroRNA (miRNA) in tumor-related epilepsy. The authors set out to correlate miR-196b expression in low-grade glioma patients with pre-operative seizures and post-operative seizure control. METHODS: Twenty-three patients with WHO grade II astrocytomas and 83 similar patients for independent validation were included. Follow-up visits regarding seizure prognosis were scheduled at 6 months. MiRNA profiling was used to identify differentially expressed miRNAs. The most important miRNA was determined by quantitative reverse-transcriptase polymerase chain reaction (q-PCR) in the validation cohort. Gene ontology (GO) analysis and whole genome mRNA profiling was performed to investigate the underlying biological processes. RESULTS: Array results showed that 30 miRNAs were overexpressed and 10 miRNAs were underexpressed (with more than 2 fold change) in patients with pre-operative seizures. MiR-196b was validated in the independent validation cohort. Patients with good seizure prognosis exhibited low levels of miR-196b expression compared with those who had poor seizure prognosis in the group without pre-operative seizures. Biological processes that relate to transcription and cell cycles were over-represented in the miR-196b-associated gene expression signature. MiR-196b-associated gene expression profiling was characterized by enrichment of genes usually involved in cell proliferation. CONCLUSIONS: We have provided the first evidence that expression of miR-196b was associated with the occurrence of pre-operative seizures in low-grade gliomas, and may predict seizure prognosis in patients without pre-operative seizures. Targeted treatments that decrease endogenous levels of miR-196b might represent novel therapeutic strategies. |
| first_indexed | 2024-12-14T01:02:21Z |
| format | Article |
| id | doaj.art-c323967d50bd4e3bb5009f096bbf07a0 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T01:02:21Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c323967d50bd4e3bb5009f096bbf07a02022-12-21T23:23:11ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0179e4621810.1371/journal.pone.0046218Significance of miR-196b in tumor-related epilepsy of patients with gliomas.Gan YouWei YanWei ZhangYongzhi WangZhaoshi BaoShouwei LiShaowu LiGuilin LiYijun SongChunsheng KangTao JiangOBJECTIVES: Seizure is a common presenting symptom of primary brain tumors. There are no published studies regarding the roles of MicroRNA (miRNA) in tumor-related epilepsy. The authors set out to correlate miR-196b expression in low-grade glioma patients with pre-operative seizures and post-operative seizure control. METHODS: Twenty-three patients with WHO grade II astrocytomas and 83 similar patients for independent validation were included. Follow-up visits regarding seizure prognosis were scheduled at 6 months. MiRNA profiling was used to identify differentially expressed miRNAs. The most important miRNA was determined by quantitative reverse-transcriptase polymerase chain reaction (q-PCR) in the validation cohort. Gene ontology (GO) analysis and whole genome mRNA profiling was performed to investigate the underlying biological processes. RESULTS: Array results showed that 30 miRNAs were overexpressed and 10 miRNAs were underexpressed (with more than 2 fold change) in patients with pre-operative seizures. MiR-196b was validated in the independent validation cohort. Patients with good seizure prognosis exhibited low levels of miR-196b expression compared with those who had poor seizure prognosis in the group without pre-operative seizures. Biological processes that relate to transcription and cell cycles were over-represented in the miR-196b-associated gene expression signature. MiR-196b-associated gene expression profiling was characterized by enrichment of genes usually involved in cell proliferation. CONCLUSIONS: We have provided the first evidence that expression of miR-196b was associated with the occurrence of pre-operative seizures in low-grade gliomas, and may predict seizure prognosis in patients without pre-operative seizures. Targeted treatments that decrease endogenous levels of miR-196b might represent novel therapeutic strategies.http://europepmc.org/articles/PMC3457999?pdf=render |
| spellingShingle | Gan You Wei Yan Wei Zhang Yongzhi Wang Zhaoshi Bao Shouwei Li Shaowu Li Guilin Li Yijun Song Chunsheng Kang Tao Jiang Significance of miR-196b in tumor-related epilepsy of patients with gliomas. PLoS ONE |
| title | Significance of miR-196b in tumor-related epilepsy of patients with gliomas. |
| title_full | Significance of miR-196b in tumor-related epilepsy of patients with gliomas. |
| title_fullStr | Significance of miR-196b in tumor-related epilepsy of patients with gliomas. |
| title_full_unstemmed | Significance of miR-196b in tumor-related epilepsy of patients with gliomas. |
| title_short | Significance of miR-196b in tumor-related epilepsy of patients with gliomas. |
| title_sort | significance of mir 196b in tumor related epilepsy of patients with gliomas |
| url | http://europepmc.org/articles/PMC3457999?pdf=render |
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