Molecular Mapping of Reduced Plant Height Gene Rht24 in Bread Wheat
Height is an important trait related to plant architecture and yield potential in bread wheat (Triticum aestivum L.). We previously identified a major quantitative trait locus QPH.caas-6A flanked by simple sequence repeat markers Xbarc103 and Xwmc256 that reduced height by 8.0–10.4%. Here QPH.caas-6...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-08-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.01379/full |
| _version_ | 1811235035281358848 |
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| author | Xiuling Tian Xiuling Tian Weie Wen Weie Wen Li Xie Luping Fu Dengan Xu Chao Fu Desen Wang Xinmin Chen Xianchun Xia Xianchun Xia Quanjia Chen Zhonghu He Zhonghu He Shuanghe Cao |
| author_facet | Xiuling Tian Xiuling Tian Weie Wen Weie Wen Li Xie Luping Fu Dengan Xu Chao Fu Desen Wang Xinmin Chen Xianchun Xia Xianchun Xia Quanjia Chen Zhonghu He Zhonghu He Shuanghe Cao |
| author_sort | Xiuling Tian |
| collection | DOAJ |
| description | Height is an important trait related to plant architecture and yield potential in bread wheat (Triticum aestivum L.). We previously identified a major quantitative trait locus QPH.caas-6A flanked by simple sequence repeat markers Xbarc103 and Xwmc256 that reduced height by 8.0–10.4%. Here QPH.caas-6A, designated as Rht24, was confirmed using recombinant inbred lines (RILs) derived from a Jingdong 8/Aikang 58 cross. The target sequences of Xbarc103 and Xwmc256 were used as queries to BLAST against International Wheat Genome Sequence Consortium database and hit a super scaffold of approximately 208 Mb. Based on gene annotation of the scaffold, three gene-specific markers were developed to genotype the RILs, and Rht24 was narrowed to a 1.85 cM interval between TaAP2 and TaFAR. In addition, three single nucleotide polymorphism (SNP) markers linked to Rht24 were identified from SNP chip-based screening in combination with bulked segregant analysis. The allelic efficacy of Rht24 was validated in 242 elite wheat varieties using TaAP2 and TaFAR markers. These showed a significant association between genotypes and plant height. Rht24 reduced plant height by an average of 6.0–7.9 cm across environments and were significantly associated with an increased TGW of 2.0–3.4 g. The findings indicate that Rht24 is a common dwarfing gene in wheat breeding, and TaAP2 and TaFAR can be used for marker-assisted selection. |
| first_indexed | 2024-04-12T11:45:29Z |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T11:45:29Z |
| publishDate | 2017-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-c327c73fa82e493494b13128e6abe7862022-12-22T03:34:22ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-08-01810.3389/fpls.2017.01379254212Molecular Mapping of Reduced Plant Height Gene Rht24 in Bread WheatXiuling Tian0Xiuling Tian1Weie Wen2Weie Wen3Li Xie4Luping Fu5Dengan Xu6Chao Fu7Desen Wang8Xinmin Chen9Xianchun Xia10Xianchun Xia11Quanjia Chen12Zhonghu He13Zhonghu He14Shuanghe Cao15College of Agronomy, Xinjiang Agricultural UniversityUrumqi, ChinaInstitute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural SciencesBeijing, ChinaCollege of Agronomy, Xinjiang Agricultural UniversityUrumqi, ChinaInstitute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural SciencesBeijing, ChinaInstitute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural SciencesBeijing, ChinaInstitute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural SciencesBeijing, ChinaInstitute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural SciencesBeijing, ChinaInstitute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural SciencesBeijing, ChinaInstitute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural SciencesBeijing, ChinaInstitute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural SciencesBeijing, ChinaCollege of Agronomy, Xinjiang Agricultural UniversityUrumqi, ChinaInstitute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural SciencesBeijing, ChinaCollege of Agronomy, Xinjiang Agricultural UniversityUrumqi, ChinaInstitute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural SciencesBeijing, ChinaInternational Maize and Wheat Improvement Center (CIMMYT)Beijing, ChinaInstitute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural SciencesBeijing, ChinaHeight is an important trait related to plant architecture and yield potential in bread wheat (Triticum aestivum L.). We previously identified a major quantitative trait locus QPH.caas-6A flanked by simple sequence repeat markers Xbarc103 and Xwmc256 that reduced height by 8.0–10.4%. Here QPH.caas-6A, designated as Rht24, was confirmed using recombinant inbred lines (RILs) derived from a Jingdong 8/Aikang 58 cross. The target sequences of Xbarc103 and Xwmc256 were used as queries to BLAST against International Wheat Genome Sequence Consortium database and hit a super scaffold of approximately 208 Mb. Based on gene annotation of the scaffold, three gene-specific markers were developed to genotype the RILs, and Rht24 was narrowed to a 1.85 cM interval between TaAP2 and TaFAR. In addition, three single nucleotide polymorphism (SNP) markers linked to Rht24 were identified from SNP chip-based screening in combination with bulked segregant analysis. The allelic efficacy of Rht24 was validated in 242 elite wheat varieties using TaAP2 and TaFAR markers. These showed a significant association between genotypes and plant height. Rht24 reduced plant height by an average of 6.0–7.9 cm across environments and were significantly associated with an increased TGW of 2.0–3.4 g. The findings indicate that Rht24 is a common dwarfing gene in wheat breeding, and TaAP2 and TaFAR can be used for marker-assisted selection.http://journal.frontiersin.org/article/10.3389/fpls.2017.01379/fullCAPS markergenome miningRht24SNP chipTriticum aestivum |
| spellingShingle | Xiuling Tian Xiuling Tian Weie Wen Weie Wen Li Xie Luping Fu Dengan Xu Chao Fu Desen Wang Xinmin Chen Xianchun Xia Xianchun Xia Quanjia Chen Zhonghu He Zhonghu He Shuanghe Cao Molecular Mapping of Reduced Plant Height Gene Rht24 in Bread Wheat Frontiers in Plant Science CAPS marker genome mining Rht24 SNP chip Triticum aestivum |
| title | Molecular Mapping of Reduced Plant Height Gene Rht24 in Bread Wheat |
| title_full | Molecular Mapping of Reduced Plant Height Gene Rht24 in Bread Wheat |
| title_fullStr | Molecular Mapping of Reduced Plant Height Gene Rht24 in Bread Wheat |
| title_full_unstemmed | Molecular Mapping of Reduced Plant Height Gene Rht24 in Bread Wheat |
| title_short | Molecular Mapping of Reduced Plant Height Gene Rht24 in Bread Wheat |
| title_sort | molecular mapping of reduced plant height gene rht24 in bread wheat |
| topic | CAPS marker genome mining Rht24 SNP chip Triticum aestivum |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.01379/full |
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