A Functional Network of Novel Barley MicroRNAs and Their Targets in Response to Drought
The regulation of mRNA (messenger RNA) levels by microRNA-mediated activity is especially important in plant responses to environmental stresses. In this work, we report six novel barley microRNAs, including two processed from the same precursor that are severely downregulated under drought conditio...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/11/5/488 |
| _version_ | 1797569287729184768 |
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| author | Aleksandra Smoczynska Andrzej M. Pacak Przemysław Nuc Aleksandra Swida-Barteczka Katarzyna Kruszka Wojciech M. Karlowski Artur Jarmolowski Zofia Szweykowska-Kulinska |
| author_facet | Aleksandra Smoczynska Andrzej M. Pacak Przemysław Nuc Aleksandra Swida-Barteczka Katarzyna Kruszka Wojciech M. Karlowski Artur Jarmolowski Zofia Szweykowska-Kulinska |
| author_sort | Aleksandra Smoczynska |
| collection | DOAJ |
| description | The regulation of mRNA (messenger RNA) levels by microRNA-mediated activity is especially important in plant responses to environmental stresses. In this work, we report six novel barley microRNAs, including two processed from the same precursor that are severely downregulated under drought conditions. For all analyzed microRNAs, we found target genes that were upregulated under drought conditions and that were known to be involved in a plethora of processes from disease resistance to chromatin–protein complex formation and the regulation of transcription in mitochondria. Targets for novel barley microRNAs were confirmed through degradome data analysis and RT-qPCR using primers flanking microRNA-recognition site. Our results show a broad transcriptional response of barley to water deficiency conditions through microRNA-mediated gene regulation and facilitate further research on drought tolerance in crops. |
| first_indexed | 2024-03-10T20:09:49Z |
| format | Article |
| id | doaj.art-c329e78beb6644f89bbd69efe6c98f1a |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T20:09:49Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-c329e78beb6644f89bbd69efe6c98f1a2023-11-19T23:03:14ZengMDPI AGGenes2073-44252020-04-0111548810.3390/genes11050488A Functional Network of Novel Barley MicroRNAs and Their Targets in Response to DroughtAleksandra Smoczynska0Andrzej M. Pacak1Przemysław Nuc2Aleksandra Swida-Barteczka3Katarzyna Kruszka4Wojciech M. Karlowski5Artur Jarmolowski6Zofia Szweykowska-Kulinska7Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, 61-614 Poznan, PolandDepartment of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, 61-614 Poznan, PolandDepartment of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, 61-614 Poznan, PolandDepartment of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, 61-614 Poznan, PolandDepartment of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, 61-614 Poznan, PolandDepartment of Computational Biology, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, 61-712 Poznan, PolandDepartment of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, 61-614 Poznan, PolandDepartment of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, 61-614 Poznan, PolandThe regulation of mRNA (messenger RNA) levels by microRNA-mediated activity is especially important in plant responses to environmental stresses. In this work, we report six novel barley microRNAs, including two processed from the same precursor that are severely downregulated under drought conditions. For all analyzed microRNAs, we found target genes that were upregulated under drought conditions and that were known to be involved in a plethora of processes from disease resistance to chromatin–protein complex formation and the regulation of transcription in mitochondria. Targets for novel barley microRNAs were confirmed through degradome data analysis and RT-qPCR using primers flanking microRNA-recognition site. Our results show a broad transcriptional response of barley to water deficiency conditions through microRNA-mediated gene regulation and facilitate further research on drought tolerance in crops.https://www.mdpi.com/2073-4425/11/5/488microRNAbarleynovel barley microRNAsmicroRNA expression patternmRNA targets of novel barley microRNAsplant response to drought |
| spellingShingle | Aleksandra Smoczynska Andrzej M. Pacak Przemysław Nuc Aleksandra Swida-Barteczka Katarzyna Kruszka Wojciech M. Karlowski Artur Jarmolowski Zofia Szweykowska-Kulinska A Functional Network of Novel Barley MicroRNAs and Their Targets in Response to Drought Genes microRNA barley novel barley microRNAs microRNA expression pattern mRNA targets of novel barley microRNAs plant response to drought |
| title | A Functional Network of Novel Barley MicroRNAs and Their Targets in Response to Drought |
| title_full | A Functional Network of Novel Barley MicroRNAs and Their Targets in Response to Drought |
| title_fullStr | A Functional Network of Novel Barley MicroRNAs and Their Targets in Response to Drought |
| title_full_unstemmed | A Functional Network of Novel Barley MicroRNAs and Their Targets in Response to Drought |
| title_short | A Functional Network of Novel Barley MicroRNAs and Their Targets in Response to Drought |
| title_sort | functional network of novel barley micrornas and their targets in response to drought |
| topic | microRNA barley novel barley microRNAs microRNA expression pattern mRNA targets of novel barley microRNAs plant response to drought |
| url | https://www.mdpi.com/2073-4425/11/5/488 |
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