Shiga toxin-producing Escherichia coli O157:H7 in milk and milk products in Ogun State, Nigeria
Shiga toxin producing Escherichia coli (STEC) O157 is a major cause of food-borne illnesses in humans. This study investigated the presence of STEC O157 in milk and milk products in Ogun State, Nigeria. Of a total of 202 samples 10 (5%) were positive for STEC O157 including 1 (2%) of 50 raw milk sam...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
2014-09-01
|
| Series: | Veterinaria Italiana |
| Subjects: | |
| Online Access: | http://www.izs.it/vet_italiana/2014/50_3/VetIt_129_2187_1.pdf |
| _version_ | 1811191986374311936 |
|---|---|
| author | Akhigbe Ivbade Olufemi Ernest Ojo Morenike Atinuke Dipeolu |
| author_facet | Akhigbe Ivbade Olufemi Ernest Ojo Morenike Atinuke Dipeolu |
| author_sort | Akhigbe Ivbade |
| collection | DOAJ |
| description | Shiga toxin producing Escherichia coli (STEC) O157 is a major cause of food-borne illnesses in humans. This study investigated the presence of STEC O157 in milk and milk products in Ogun State, Nigeria. Of a total of 202 samples 10 (5%) were positive for STEC O157 including 1 (2%) of 50 raw milk samples, 3 (6%) of 50 samples of fresh local cheese, 1 (2%) of 50 samples of fried local cheese and 5 (9.6%) of 52 fermented milk samples. There was no significant difference (p>0.05) in the prevalence of STEC O157 among the sample types. Of 10 isolates, shiga toxin 1 gene (stx1) was detected only in 2 samples (20%), shiga toxin 2 (stx2) was extracted only in 6 samples (60%), stx1 /stx2 in 2 samples (20.0%), intimin gene (eaeA) in 5 samples (50%), and enterohaemolysin (E-hlyA) gene was isolated in 7 (70%) samples. Rates of resistance of the STEC O157 isolates were: amoxicillin/clavulanic acid 100%, ampicillin 100%, chloramphenicol 60%, nalidixic acid 20%, norfloxacin 10%, streptomycin 30%, sulphamethoxazole/trimethprim 20%, and tetracycline 90%. The isolates were all susceptible to ciprofloxacin and neomycin. The presence of virulent multidrug resistant E. coli O157 strains in milk and milk products as revealed by this study unveils a risk of human exposure to these potentially fatal pathogens following consumption of contaminated products. |
| first_indexed | 2024-04-11T23:44:48Z |
| format | Article |
| id | doaj.art-c32b485cb0fc49e6a9099cfa4b276586 |
| institution | Directory Open Access Journal |
| issn | 0505-401X 1828-1427 |
| language | English |
| last_indexed | 2024-04-11T23:44:48Z |
| publishDate | 2014-09-01 |
| publisher | Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale |
| record_format | Article |
| series | Veterinaria Italiana |
| spelling | doaj.art-c32b485cb0fc49e6a9099cfa4b2765862022-12-22T03:56:40ZengIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. CaporaleVeterinaria Italiana0505-401X1828-14272014-09-0150318519110.12834/VetIt.129.2187.1Shiga toxin-producing Escherichia coli O157:H7 in milk and milk products in Ogun State, NigeriaAkhigbe Ivbade0Olufemi Ernest Ojo1Morenike Atinuke Dipeolu2Department of Veterinary Public Health and Reproduction, College of Veterinary Medicine, Federal University of Agriculture Abeokuta, Abeokuta, NigeriaDepartment of Veterinary Microbiology and Parasitology, College of Veterinary Medicine, Federal University of Agriculture Abeokuta, Abeokuta, Ogun State, Nigeria. Tel.: +234 803 5803 716, e-mail: oeoefemi@yahoo.com, ojooe@funaab.edu.ngDepartment of Veterinary Public Health and Reproduction, College of Veterinary Medicine, Federal University of Agriculture Abeokuta, Abeokuta, NigeriaShiga toxin producing Escherichia coli (STEC) O157 is a major cause of food-borne illnesses in humans. This study investigated the presence of STEC O157 in milk and milk products in Ogun State, Nigeria. Of a total of 202 samples 10 (5%) were positive for STEC O157 including 1 (2%) of 50 raw milk samples, 3 (6%) of 50 samples of fresh local cheese, 1 (2%) of 50 samples of fried local cheese and 5 (9.6%) of 52 fermented milk samples. There was no significant difference (p>0.05) in the prevalence of STEC O157 among the sample types. Of 10 isolates, shiga toxin 1 gene (stx1) was detected only in 2 samples (20%), shiga toxin 2 (stx2) was extracted only in 6 samples (60%), stx1 /stx2 in 2 samples (20.0%), intimin gene (eaeA) in 5 samples (50%), and enterohaemolysin (E-hlyA) gene was isolated in 7 (70%) samples. Rates of resistance of the STEC O157 isolates were: amoxicillin/clavulanic acid 100%, ampicillin 100%, chloramphenicol 60%, nalidixic acid 20%, norfloxacin 10%, streptomycin 30%, sulphamethoxazole/trimethprim 20%, and tetracycline 90%. The isolates were all susceptible to ciprofloxacin and neomycin. The presence of virulent multidrug resistant E. coli O157 strains in milk and milk products as revealed by this study unveils a risk of human exposure to these potentially fatal pathogens following consumption of contaminated products.http://www.izs.it/vet_italiana/2014/50_3/VetIt_129_2187_1.pdfMilkMilk productsMultidrug resistanceNigeriaSTEC O157Virulence genes |
| spellingShingle | Akhigbe Ivbade Olufemi Ernest Ojo Morenike Atinuke Dipeolu Shiga toxin-producing Escherichia coli O157:H7 in milk and milk products in Ogun State, Nigeria Veterinaria Italiana Milk Milk products Multidrug resistance Nigeria STEC O157 Virulence genes |
| title | Shiga toxin-producing Escherichia coli O157:H7 in milk and milk products in Ogun State, Nigeria |
| title_full | Shiga toxin-producing Escherichia coli O157:H7 in milk and milk products in Ogun State, Nigeria |
| title_fullStr | Shiga toxin-producing Escherichia coli O157:H7 in milk and milk products in Ogun State, Nigeria |
| title_full_unstemmed | Shiga toxin-producing Escherichia coli O157:H7 in milk and milk products in Ogun State, Nigeria |
| title_short | Shiga toxin-producing Escherichia coli O157:H7 in milk and milk products in Ogun State, Nigeria |
| title_sort | shiga toxin producing escherichia coli o157 h7 in milk and milk products in ogun state nigeria |
| topic | Milk Milk products Multidrug resistance Nigeria STEC O157 Virulence genes |
| url | http://www.izs.it/vet_italiana/2014/50_3/VetIt_129_2187_1.pdf |
| work_keys_str_mv | AT akhigbeivbade shigatoxinproducingescherichiacolio157h7inmilkandmilkproductsinogunstatenigeria AT olufemiernestojo shigatoxinproducingescherichiacolio157h7inmilkandmilkproductsinogunstatenigeria AT morenikeatinukedipeolu shigatoxinproducingescherichiacolio157h7inmilkandmilkproductsinogunstatenigeria |