Ultrasensitive colorimetric detection of fluoride and arsenate in water and mammalian cells using recyclable metal oxacalixarene probe: a lateral flow assay
Abstract Globally 3 billion people are consuming water with moderately high concentrations of fluoride and arsenic. The development of a simple point of care (PoC) device or home device for the detection of fluoride/arsenic ensures safety before consuming water. Till date, lateral flow assay (LFA) b...
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Nature Portfolio
2022-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-21407-w |
| _version_ | 1817984030048518144 |
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| author | Shuvankar Dey Anshu Kumar Pradip Kumar Mondal Deepak Chopra Rupam Roy Sana Jindani Bishwajit Ganguly Chaithra Mayya Dhiraj Bhatia Vinod K. Jain |
| author_facet | Shuvankar Dey Anshu Kumar Pradip Kumar Mondal Deepak Chopra Rupam Roy Sana Jindani Bishwajit Ganguly Chaithra Mayya Dhiraj Bhatia Vinod K. Jain |
| author_sort | Shuvankar Dey |
| collection | DOAJ |
| description | Abstract Globally 3 billion people are consuming water with moderately high concentrations of fluoride and arsenic. The development of a simple point of care (PoC) device or home device for the detection of fluoride/arsenic ensures safety before consuming water. Till date, lateral flow assay (LFA) based PoC devices can detect nucleic acids, viruses and diseases. An aluminium complex of rhodamine B functionalized oxacalix[4]arene (L) was designed to execute the LFA-based PoC device. Initially, Al3+ and Fe3+ ions were involved in complexation with the rhodamine B functionalized oxacalix[4]arene (L), resulting C 1 (L-Al3+) and C 2 (L-Fe3+) complexes respectively. The receptor L, as well as the probes (C 1 , C 2 ), were characterized thoroughly using mass spectroscopy, FTIR, NMR, and EA. C 1 and C 2 were further utilized as recyclable probes for the detection of aqueous fluoride (21 ppb) and arsenate (1.92 ppb) respectively. The computational calculation indicates that upon complexation, the spirolactam ring opening at the rhodamine B site leads to optoelectronic changes. The consistency of LFA-based portable sensing device has been tested with water samples, synthetic fluoride standards and dental care products like toothpaste and mouthwash with concentrations ≥ 3 ppm. Moreover, fixed cell imaging experiments were performed to ascertain the in-vitro sensing phenomena. |
| first_indexed | 2024-04-13T23:40:10Z |
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| id | doaj.art-c32bd9ae35024892b9a7dff3a5bde678 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T23:40:10Z |
| publishDate | 2022-10-01 |
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| series | Scientific Reports |
| spelling | doaj.art-c32bd9ae35024892b9a7dff3a5bde6782022-12-22T02:24:33ZengNature PortfolioScientific Reports2045-23222022-10-0112111610.1038/s41598-022-21407-wUltrasensitive colorimetric detection of fluoride and arsenate in water and mammalian cells using recyclable metal oxacalixarene probe: a lateral flow assayShuvankar Dey0Anshu Kumar1Pradip Kumar Mondal2Deepak Chopra3Rupam Roy4Sana Jindani5Bishwajit Ganguly6Chaithra Mayya7Dhiraj Bhatia8Vinod K. Jain9Department of Chemistry, School of Sciences, Gujarat UniversityDepartment of Chemistry, School of Sciences, Gujarat UniversityElettra-Sincrotrone TriesteDepartment of Chemistry, Indian Institute of Science Education and Research BhopalDepartment of Chemistry, Indian Institute of Science Education and Research BhopalComputation and Simulation Unit (Analytical and Environmental Science Discipline and Centralized Instrument Facility), CSIR-Central Salt and Marine Chemicals Research InstituteComputation and Simulation Unit (Analytical and Environmental Science Discipline and Centralized Instrument Facility), CSIR-Central Salt and Marine Chemicals Research InstituteBiological Engineering Discipline, Indian Institute of TechnologyBiological Engineering Discipline, Indian Institute of TechnologyDepartment of Chemistry, School of Sciences, Gujarat UniversityAbstract Globally 3 billion people are consuming water with moderately high concentrations of fluoride and arsenic. The development of a simple point of care (PoC) device or home device for the detection of fluoride/arsenic ensures safety before consuming water. Till date, lateral flow assay (LFA) based PoC devices can detect nucleic acids, viruses and diseases. An aluminium complex of rhodamine B functionalized oxacalix[4]arene (L) was designed to execute the LFA-based PoC device. Initially, Al3+ and Fe3+ ions were involved in complexation with the rhodamine B functionalized oxacalix[4]arene (L), resulting C 1 (L-Al3+) and C 2 (L-Fe3+) complexes respectively. The receptor L, as well as the probes (C 1 , C 2 ), were characterized thoroughly using mass spectroscopy, FTIR, NMR, and EA. C 1 and C 2 were further utilized as recyclable probes for the detection of aqueous fluoride (21 ppb) and arsenate (1.92 ppb) respectively. The computational calculation indicates that upon complexation, the spirolactam ring opening at the rhodamine B site leads to optoelectronic changes. The consistency of LFA-based portable sensing device has been tested with water samples, synthetic fluoride standards and dental care products like toothpaste and mouthwash with concentrations ≥ 3 ppm. Moreover, fixed cell imaging experiments were performed to ascertain the in-vitro sensing phenomena.https://doi.org/10.1038/s41598-022-21407-w |
| spellingShingle | Shuvankar Dey Anshu Kumar Pradip Kumar Mondal Deepak Chopra Rupam Roy Sana Jindani Bishwajit Ganguly Chaithra Mayya Dhiraj Bhatia Vinod K. Jain Ultrasensitive colorimetric detection of fluoride and arsenate in water and mammalian cells using recyclable metal oxacalixarene probe: a lateral flow assay Scientific Reports |
| title | Ultrasensitive colorimetric detection of fluoride and arsenate in water and mammalian cells using recyclable metal oxacalixarene probe: a lateral flow assay |
| title_full | Ultrasensitive colorimetric detection of fluoride and arsenate in water and mammalian cells using recyclable metal oxacalixarene probe: a lateral flow assay |
| title_fullStr | Ultrasensitive colorimetric detection of fluoride and arsenate in water and mammalian cells using recyclable metal oxacalixarene probe: a lateral flow assay |
| title_full_unstemmed | Ultrasensitive colorimetric detection of fluoride and arsenate in water and mammalian cells using recyclable metal oxacalixarene probe: a lateral flow assay |
| title_short | Ultrasensitive colorimetric detection of fluoride and arsenate in water and mammalian cells using recyclable metal oxacalixarene probe: a lateral flow assay |
| title_sort | ultrasensitive colorimetric detection of fluoride and arsenate in water and mammalian cells using recyclable metal oxacalixarene probe a lateral flow assay |
| url | https://doi.org/10.1038/s41598-022-21407-w |
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