Highly Specific and Cost-Efficient Detection of Salmonella Paratyphi A Combining Aptamers with Single-Walled Carbon Nanotubes
In this paper, a panel of single-stranded DNA aptamers with high affinity and specificity against Salmonella Paratyphi A was selected from an enriched oligonucleotide pool by a whole-cell-Systematic Evolution of Ligands by Exponential Enrichment (SELEX) procedure, during which four other Salmonella...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2013-05-01
|
| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/13/5/6865 |
| _version_ | 1811279318935928832 |
|---|---|
| author | Le Deng Qin Zhou Yongzhe Chen Zhihui Peng Yi Ning Ming Yang |
| author_facet | Le Deng Qin Zhou Yongzhe Chen Zhihui Peng Yi Ning Ming Yang |
| author_sort | Le Deng |
| collection | DOAJ |
| description | In this paper, a panel of single-stranded DNA aptamers with high affinity and specificity against Salmonella Paratyphi A was selected from an enriched oligonucleotide pool by a whole-cell-Systematic Evolution of Ligands by Exponential Enrichment (SELEX) procedure, during which four other Salmonella serovars were used as counter-selection targets. It was determined through a fluorescence assay that the selected aptamers had high binding ability and specificity to this pathogen. The dissociation constant of these aptamers were up to nanomolar range, and aptamer Apt22 with the lowest Kd (47 ± 3 nM) was used in cell imaging experiments. To detect this bacteria with high specificity and cost-efficiently, a novel useful detection method was also constructed based on the noncovalent self-assembly of single-walled carbon nanotubes (SWNTs) and DNAzyme-labeled aptamer detection probes. The amounts of target bacteria could be quantified by exploiting chemoluminescence intensity changes at 420 nm and the detection limit of the method was 103 cfu/mL. This study demonstrated the applicability of Salmonella specific aptamers and their potential for use in the detection of Salmonella in food, clinical and environmental samples. |
| first_indexed | 2024-04-13T00:52:32Z |
| format | Article |
| id | doaj.art-c32c6eebb6f7480aa09e738ee40bcb93 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-13T00:52:32Z |
| publishDate | 2013-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-c32c6eebb6f7480aa09e738ee40bcb932022-12-22T03:09:51ZengMDPI AGSensors1424-82202013-05-011356865688110.3390/s130506865Highly Specific and Cost-Efficient Detection of Salmonella Paratyphi A Combining Aptamers with Single-Walled Carbon NanotubesLe DengQin ZhouYongzhe ChenZhihui PengYi NingMing YangIn this paper, a panel of single-stranded DNA aptamers with high affinity and specificity against Salmonella Paratyphi A was selected from an enriched oligonucleotide pool by a whole-cell-Systematic Evolution of Ligands by Exponential Enrichment (SELEX) procedure, during which four other Salmonella serovars were used as counter-selection targets. It was determined through a fluorescence assay that the selected aptamers had high binding ability and specificity to this pathogen. The dissociation constant of these aptamers were up to nanomolar range, and aptamer Apt22 with the lowest Kd (47 ± 3 nM) was used in cell imaging experiments. To detect this bacteria with high specificity and cost-efficiently, a novel useful detection method was also constructed based on the noncovalent self-assembly of single-walled carbon nanotubes (SWNTs) and DNAzyme-labeled aptamer detection probes. The amounts of target bacteria could be quantified by exploiting chemoluminescence intensity changes at 420 nm and the detection limit of the method was 103 cfu/mL. This study demonstrated the applicability of Salmonella specific aptamers and their potential for use in the detection of Salmonella in food, clinical and environmental samples.http://www.mdpi.com/1424-8220/13/5/6865Salmonella Paratyphi Aaptamersingle-walled carbon nanotubesdetection |
| spellingShingle | Le Deng Qin Zhou Yongzhe Chen Zhihui Peng Yi Ning Ming Yang Highly Specific and Cost-Efficient Detection of Salmonella Paratyphi A Combining Aptamers with Single-Walled Carbon Nanotubes Sensors Salmonella Paratyphi A aptamer single-walled carbon nanotubes detection |
| title | Highly Specific and Cost-Efficient Detection of Salmonella Paratyphi A Combining Aptamers with Single-Walled Carbon Nanotubes |
| title_full | Highly Specific and Cost-Efficient Detection of Salmonella Paratyphi A Combining Aptamers with Single-Walled Carbon Nanotubes |
| title_fullStr | Highly Specific and Cost-Efficient Detection of Salmonella Paratyphi A Combining Aptamers with Single-Walled Carbon Nanotubes |
| title_full_unstemmed | Highly Specific and Cost-Efficient Detection of Salmonella Paratyphi A Combining Aptamers with Single-Walled Carbon Nanotubes |
| title_short | Highly Specific and Cost-Efficient Detection of Salmonella Paratyphi A Combining Aptamers with Single-Walled Carbon Nanotubes |
| title_sort | highly specific and cost efficient detection of salmonella paratyphi a combining aptamers with single walled carbon nanotubes |
| topic | Salmonella Paratyphi A aptamer single-walled carbon nanotubes detection |
| url | http://www.mdpi.com/1424-8220/13/5/6865 |
| work_keys_str_mv | AT ledeng highlyspecificandcostefficientdetectionofsalmonellaparatyphiacombiningaptamerswithsinglewalledcarbonnanotubes AT qinzhou highlyspecificandcostefficientdetectionofsalmonellaparatyphiacombiningaptamerswithsinglewalledcarbonnanotubes AT yongzhechen highlyspecificandcostefficientdetectionofsalmonellaparatyphiacombiningaptamerswithsinglewalledcarbonnanotubes AT zhihuipeng highlyspecificandcostefficientdetectionofsalmonellaparatyphiacombiningaptamerswithsinglewalledcarbonnanotubes AT yining highlyspecificandcostefficientdetectionofsalmonellaparatyphiacombiningaptamerswithsinglewalledcarbonnanotubes AT mingyang highlyspecificandcostefficientdetectionofsalmonellaparatyphiacombiningaptamerswithsinglewalledcarbonnanotubes |